FastQCFastQC Report
Fri 10 Feb 2017
ERR1630540.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630540.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964023
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT132881.3783903496078413No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT130501.3537021419613433No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT106841.10827231300498No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69870.7247752387650502No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA28490.29553236800366794No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC25570.2652426342525023No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC24200.2510313550610307No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA22080.22904017850196523No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19800.20538929050447968No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG19690.2042482388905659No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC18750.19449743418984816No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17710.18370930984011793No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC17450.18101227875268536No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC15740.1632741127545712No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15560.16140693738634868No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG15510.1608882775618424No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC15070.1563240711061873No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC14640.15186359661543344No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC14390.14927029749290216No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC13840.14356503942333326No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG13250.13744485349415939No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG12850.13329557489810928No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT12550.13018361595107172No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT12130.12582687342521912No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT11880.1232335743026878No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11610.12043281125035399No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC11080.11493501711058761No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC11020.11431262532118008No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG11020.11431262532118008No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG11010.11420889335627883No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT10580.109748418865525No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC10570.10964468690062375No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG10500.10891856314631497No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC10440.10829617135690746No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC10390.1077775115324012No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG10090.10466555258536363No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG9990.1036282329363511No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT9830.10196852149793106No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC9770.10134612970852355No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATGC250.005495908729.63
ATCCGAC405.9391525E-527.7527
GCGGACC451.3229957E-424.66666814
GAGGCAC2100.024.6666667
GTACAAG400.001930962823.1251
AACCTAA400.001930962823.12519
ATTAGAG2300.022.521743
TATTAGA2400.022.3541662
TCCGACT502.701664E-422.228
TGCGGAC502.701664E-422.213
GCAGCGT502.701664E-422.21
CGGACCA502.701664E-422.215
CTATGCT705.1005154E-621.1428574
CACATGT2400.020.81249828
GACACAT2500.020.72000126
CGTATTA450.003825366520.55555715
GAGTCGC450.003825366520.5555578
ACGTGGT450.003825366520.55555711
TCCGTAT450.003825366520.55555713
CGTACAT450.003825366520.55555712
Produced by FastQC (version 0.11.5)