##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630540.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	964023
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.642687985660093	31.0	31.0	34.0	30.0	34.0
2	31.780854813629965	31.0	31.0	34.0	30.0	34.0
3	31.82689002233349	33.0	31.0	34.0	30.0	34.0
4	35.52494286962033	37.0	35.0	37.0	33.0	37.0
5	35.49314072382091	37.0	35.0	37.0	33.0	37.0
6	35.60843050425145	37.0	35.0	37.0	33.0	37.0
7	35.54772863303054	37.0	35.0	37.0	33.0	37.0
8	35.58533873154479	37.0	35.0	37.0	33.0	37.0
9	37.16366310762295	39.0	37.0	39.0	34.0	39.0
10	37.036631906085226	39.0	37.0	39.0	33.0	39.0
11	37.18636692278089	39.0	37.0	39.0	34.0	39.0
12	37.1299958610946	39.0	37.0	39.0	33.0	39.0
13	37.18865836188556	39.0	37.0	39.0	34.0	39.0
14	38.293553162113355	40.0	38.0	41.0	34.0	41.0
15	38.26454763008766	40.0	38.0	41.0	34.0	41.0
16	38.172703348364095	40.0	37.0	41.0	33.0	41.0
17	38.23615722861384	40.0	38.0	41.0	33.0	41.0
18	38.24602421311525	40.0	38.0	41.0	33.0	41.0
19	38.358563021836616	40.0	38.0	41.0	34.0	41.0
20	38.32114482745744	40.0	38.0	41.0	34.0	41.0
21	38.275020409264094	40.0	38.0	41.0	34.0	41.0
22	38.179583889595996	40.0	38.0	41.0	34.0	41.0
23	38.061497495391706	40.0	37.0	41.0	33.0	41.0
24	37.98485409580477	40.0	37.0	41.0	33.0	41.0
25	37.90500849046133	40.0	37.0	41.0	33.0	41.0
26	37.67666850272244	40.0	37.0	41.0	33.0	41.0
27	37.48370215233454	39.0	36.0	41.0	32.0	41.0
28	37.30060278644804	39.0	36.0	41.0	32.0	41.0
29	37.18721441293413	39.0	36.0	41.0	32.0	41.0
30	36.98864134984331	39.0	35.0	40.0	31.0	41.0
31	36.87203002417992	39.0	35.0	40.0	31.0	41.0
32	36.67645274023545	39.0	35.0	40.0	31.0	41.0
33	36.47866596543859	39.0	35.0	40.0	30.0	41.0
34	36.34112775317601	38.0	35.0	40.0	30.0	41.0
35	36.14470194175865	38.0	35.0	40.0	30.0	41.0
36	36.05360349286272	38.0	35.0	40.0	30.0	41.0
37	35.78183508069828	38.0	35.0	40.0	28.0	41.0
38	35.51431864177514	38.0	35.0	40.0	27.0	41.0
39	35.32570177267555	38.0	35.0	40.0	26.0	41.0
40	34.992317610679414	38.0	34.0	40.0	24.0	41.0
41	34.79842389652529	38.0	34.0	40.0	23.0	41.0
42	34.45156080300988	38.0	33.0	40.0	22.0	41.0
43	33.62773606023923	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	8.0
11	10.0
12	4.0
13	6.0
14	4.0
15	4.0
16	6.0
17	25.0
18	69.0
19	115.0
20	297.0
21	552.0
22	1006.0
23	1790.0
24	2712.0
25	4239.0
26	6362.0
27	9162.0
28	12758.0
29	17198.0
30	21775.0
31	27082.0
32	33112.0
33	41619.0
34	53058.0
35	68584.0
36	96374.0
37	152748.0
38	234237.0
39	179104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.99693160847822	20.299411943490973	12.80280657204237	20.900849875988435
2	20.10595182895014	21.651246910084097	33.687266797576406	24.555534463389357
3	22.568963603565475	26.533495570126437	28.213953401526727	22.683587424781358
4	13.504138386739736	16.249301105886477	33.972944628914455	36.27361587845933
5	12.29120052114939	41.22256419193318	32.60285283649871	13.883382450418713
6	32.168008439632665	34.118480575670915	16.89627737097559	16.817233613720834
7	27.495090884761048	32.49279322173849	21.769501350071526	18.242614543428942
8	30.474480380654818	30.044407654174226	19.27007965577585	20.211032309395108
9	28.33687577993471	12.164128864145358	21.794811949507427	37.7041834064125
10	20.695875513343562	27.56127187836805	30.444398110833454	21.298454497454937
11	33.221406543204886	23.48294594631041	20.05128508344718	23.24436242703753
12	22.744893016038	29.412368792030897	26.570009221771677	21.272728970159427
13	29.741302852732765	20.282918561071675	28.255342455522324	21.72043613067323
14	22.76491328526394	22.1651350642049	27.9218441883648	27.148107462166358
15	26.7124332095811	26.426029254488743	26.393042489650142	20.468495046280015
16	23.922873209456622	25.231970606510423	27.945598808327187	22.89955737570577
17	23.053495611619223	26.665857557340438	27.069893560630813	23.210753270409523
18	21.351980191343984	23.394670044179446	31.17301143229985	24.08033833217672
19	21.93163441121218	25.14566561171258	31.44707128356896	21.475628693506273
20	22.99696169074804	22.873935580375157	32.710734079996016	21.418368648880783
21	23.349131711587795	25.082389113122822	30.95164741920058	20.616831756088807
22	24.929695660788177	23.898288733775026	30.097414688238764	21.074600917198033
23	22.702051714533784	24.16052314104539	31.114610336060444	22.02281480836038
24	20.846701790309982	26.345222053830664	30.03745761252584	22.77061854333351
25	21.810268012277714	25.196286810584397	31.37477010403279	21.618675073105102
26	21.59948465959837	26.892408168684774	30.401038149504732	21.107069022212123
27	22.50558337301081	25.796894887362647	30.454045183569274	21.243476556057274
28	20.994519840294267	25.98713931099154	31.643539625091933	21.37480122362226
29	21.082380814565628	26.054253892282652	32.03761735975179	20.82574793339993
30	19.172675340733573	27.5239283710036	31.966146035934827	21.337250252328005
31	21.79491568147233	27.712616815158974	29.619521525938698	20.87294597743
32	20.35044807022239	26.709736178493664	30.39595528322457	22.54386046805937
33	19.442170985547026	27.169268783006213	30.621572306884794	22.766987924561967
34	21.73537353361901	26.19740400384638	31.00413579344061	21.063086669093995
35	19.844651009363883	27.771640303187787	31.08276462283576	21.30094406461257
36	20.72315702011259	28.083769785575658	29.531245623807727	21.661827570504023
37	21.04016190485082	26.228523593316755	30.69148765122824	22.039826850604186
38	20.1273206137198	26.20310926191595	30.25685071829199	23.41271940607226
39	20.494013109645724	27.31822788460441	30.73412148880265	21.45363751694721
40	19.725359249727443	27.054333765895628	30.29222331832332	22.92808366605361
41	19.843821153644676	26.859940063670678	30.49927232026622	22.79696646241843
42	19.42619626295223	27.873193896826116	30.899470240855248	21.801139599366408
43	19.156389422244075	25.834238394727098	30.16795242437162	24.84141975865721
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	316.0
1	311.5
2	307.0
3	898.0
4	1489.0
5	1489.0
6	2380.0
7	3271.0
8	3420.5
9	3570.0
10	5131.5
11	6693.0
12	6693.0
13	11947.5
14	17202.0
15	23741.5
16	30281.0
17	27521.0
18	24761.0
19	24761.0
20	25402.5
21	26044.0
22	18983.5
23	11923.0
24	10724.0
25	9525.0
26	9525.0
27	10437.0
28	11349.0
29	13398.0
30	15447.0
31	18317.5
32	21188.0
33	21188.0
34	25986.5
35	30785.0
36	33833.5
37	36882.0
38	43934.5
39	50987.0
40	50987.0
41	54469.0
42	57951.0
43	62655.5
44	67360.0
45	68217.0
46	69074.0
47	69074.0
48	72745.0
49	76416.0
50	73588.0
51	70760.0
52	69852.0
53	68944.0
54	68944.0
55	61602.0
56	54260.0
57	47027.0
58	39794.0
59	37954.5
60	36115.0
61	36115.0
62	34749.0
63	33383.0
64	30695.5
65	28008.0
66	26764.0
67	25520.0
68	25520.0
69	19745.0
70	13970.0
71	12160.0
72	10350.0
73	7196.0
74	4042.0
75	4042.0
76	3149.5
77	2257.0
78	1782.0
79	1307.0
80	1094.5
81	882.0
82	882.0
83	773.0
84	664.0
85	566.5
86	469.0
87	369.5
88	270.0
89	270.0
90	217.5
91	165.0
92	101.5
93	38.0
94	20.5
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	964023.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.418515171863497
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.69349693353752	22.647708517045924
2	8.89294440346178	5.0544855090463425
3	2.9423376824362477	2.5085060420718053
4	1.5958954035888036	1.8141191095838254
5	0.995910170765163	1.4151144148851473
6	0.7405247627992251	1.2626768524050236
7	0.553599153189462	1.1012726153831853
8	0.42152652244178845	0.9583326298683859
9	0.337844574694327	0.864093705451425
>10	2.9621488963063944	17.428944595095857
>50	0.44655080017926796	8.869881186953966
>100	0.36735952371989083	19.29698220817041
>500	0.036662627117753574	7.1971148425775455
>1k	0.011732040677681143	4.995481393839093
>5k	3.666262711775357E-4	0.7279737308378955
>10k+	0.0010998788135326073	3.8573126467841092
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13288	1.3783903496078413	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13050	1.3537021419613433	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10684	1.10827231300498	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6987	0.7247752387650502	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2849	0.29553236800366794	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	2557	0.2652426342525023	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2420	0.2510313550610307	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	2208	0.22904017850196523	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1980	0.20538929050447968	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1969	0.2042482388905659	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1875	0.19449743418984816	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1771	0.18370930984011793	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1745	0.18101227875268536	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	1574	0.1632741127545712	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1556	0.16140693738634868	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	1551	0.1608882775618424	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1507	0.1563240711061873	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1464	0.15186359661543344	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	1439	0.14927029749290216	No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC	1384	0.14356503942333326	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	1325	0.13744485349415939	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	1285	0.13329557489810928	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1255	0.13018361595107172	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	1213	0.12582687342521912	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	1188	0.1232335743026878	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1161	0.12043281125035399	No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC	1108	0.11493501711058761	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC	1102	0.11431262532118008	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	1102	0.11431262532118008	No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG	1101	0.11420889335627883	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT	1058	0.109748418865525	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	1057	0.10964468690062375	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	1050	0.10891856314631497	No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC	1044	0.10829617135690746	No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC	1039	0.1077775115324012	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1009	0.10466555258536363	No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG	999	0.1036282329363511	No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT	983	0.10196852149793106	No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC	977	0.10134612970852355	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.001452247508617533	0.0
3	0.0	0.0	0.0	0.002904495017235066	0.0
4	0.0	0.0	0.0	0.004667938420556356	0.0
5	0.0	0.0	0.0	0.004979134315260113	0.0
6	0.0	0.0	0.0	0.0051865982450626175	0.0
7	0.0	0.0	0.0	0.005912721999371384	0.0
8	1.0373196490125235E-4	0.0	0.0	0.006327649858976394	0.0
9	1.0373196490125235E-4	0.0	0.0	0.010165732560322731	0.0
10	1.0373196490125235E-4	0.0	0.0	0.014418743121274077	0.0
11	1.0373196490125235E-4	0.0	0.0	0.016908310278904135	1.0373196490125235E-4
12	1.0373196490125235E-4	0.0	0.0	0.01939787743653419	1.0373196490125235E-4
13	1.0373196490125235E-4	0.0	0.0	0.02157624869946049	1.0373196490125235E-4
14	1.0373196490125235E-4	0.0	0.0	0.024377011751794304	1.0373196490125235E-4
15	1.0373196490125235E-4	0.0	0.0	0.030082269821363184	1.0373196490125235E-4
16	1.0373196490125235E-4	0.0	0.0	0.04408608508303225	1.0373196490125235E-4
17	1.0373196490125235E-4	0.0	0.0	0.06089066339703513	1.0373196490125235E-4
18	1.0373196490125235E-4	0.0	0.0	0.06867056076462906	1.0373196490125235E-4
19	1.0373196490125235E-4	0.0	0.0	0.08049600476337183	1.0373196490125235E-4
20	1.0373196490125235E-4	0.0	0.0	0.08879456195547202	1.0373196490125235E-4
21	1.0373196490125235E-4	0.0	0.0	0.10922975904101873	2.074639298025047E-4
22	1.0373196490125235E-4	0.0	0.0	0.1456396787213583	2.074639298025047E-4
23	1.0373196490125235E-4	0.0	0.0	0.2122356001879623	2.074639298025047E-4
24	1.0373196490125235E-4	0.0	0.0	0.3035197293010644	2.074639298025047E-4
25	1.0373196490125235E-4	0.0	0.0	0.32696315336874743	2.074639298025047E-4
26	1.0373196490125235E-4	0.0	0.0	0.3659663721716183	2.074639298025047E-4
27	1.0373196490125235E-4	0.0	0.0	0.39418146662475895	2.074639298025047E-4
28	1.0373196490125235E-4	0.0	0.0	0.4412757786899275	2.074639298025047E-4
29	1.0373196490125235E-4	0.0	0.0	0.5108799271386678	2.074639298025047E-4
30	1.0373196490125235E-4	0.0	0.0	0.6312090064241206	2.074639298025047E-4
31	1.0373196490125235E-4	0.0	0.0	1.025597936978682	2.074639298025047E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTATGC	25	0.0054959087	29.6	3
ATCCGAC	40	5.9391525E-5	27.75	27
GCGGACC	45	1.3229957E-4	24.666668	14
GAGGCAC	210	0.0	24.666666	7
GTACAAG	40	0.0019309628	23.125	1
AACCTAA	40	0.0019309628	23.125	19
ATTAGAG	230	0.0	22.52174	3
TATTAGA	240	0.0	22.354166	2
TCCGACT	50	2.701664E-4	22.2	28
TGCGGAC	50	2.701664E-4	22.2	13
GCAGCGT	50	2.701664E-4	22.2	1
CGGACCA	50	2.701664E-4	22.2	15
CTATGCT	70	5.1005154E-6	21.142857	4
CACATGT	240	0.0	20.812498	28
GACACAT	250	0.0	20.720001	26
CGTATTA	45	0.0038253665	20.555557	15
GAGTCGC	45	0.0038253665	20.555557	8
ACGTGGT	45	0.0038253665	20.555557	11
TCCGTAT	45	0.0038253665	20.555557	13
CGTACAT	45	0.0038253665	20.555557	12
>>END_MODULE