FastQCFastQC Report
Fri 10 Feb 2017
ERR1630539.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630539.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences980389
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT74380.7586784429445863No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT62630.6388280570263436No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT58040.5920099062718982No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45520.4643054950636941No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13430.1369864410963403No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13050.13311042861558015No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC12250.12495040234029554No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11490.11719837737877517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAC851.2449254E-619.5882363
CGTATGC1451.546141E-1017.86206831
ATGCCGT1451.546141E-1017.86206834
CGAGTAC650.001579709417.0769253
GGACTAG650.001579709417.0769251
GCCGTCT1655.4569682E-1116.81818236
ACCTAGG4550.016.670331
TCGGTTG803.381931E-416.187524
AGACGGA1059.340212E-615.8571446
GGACCAT1059.340212E-615.8571446
AACGCCG700.002592129615.8571425
TAATACT957.0579816E-515.5789484
TAGGGTG1557.201379E-915.5161295
ACGGACC1201.9346953E-615.4166678
GACGGAC1201.9346953E-615.4166677
CGCATCG855.363152E-415.23529413
GGTATCA43500.015.0551721
ATAACGC1252.957484E-614.8000013
AGCGTCA750.004103834314.83
CCGCTTA1001.09310306E-414.825
Produced by FastQC (version 0.11.5)