FastQCFastQC Report
Fri 10 Feb 2017
ERR1630538.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630538.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409593
Sequences flagged as poor quality0
Sequence length43
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT167054.078438840507529No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT155043.7852209388344042No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT122522.9912620577011815No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94532.3079007697885463No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28880.7050901748809184No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25240.6162214686286143No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24770.6047466631509816No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA17410.4250560922672018No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG16760.40918668043643325No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT14260.34815048108732327No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14110.3444883091263767No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA13440.3281306077008152No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12580.30713415512472136No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG10370.25317815490010814No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10110.24683039016780073No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA9540.23291413671620362No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA8750.21362669772188486No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA8110.19800143068851275No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7830.19116537636141243No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT7490.18286445324993347No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA7150.17456353013845452No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA7100.17334280615147232No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT7090.17309866135407587No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC6900.1684599102035435No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA6550.15991484229466812No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA6460.15771753911810016No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA6340.1547878015493429No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT6210.15161391918318917TruSeq Adapter, Index 7 (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA6170.1506373399936034No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5280.12890845302532025No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA5210.12719943944354517No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA5170.12622286025395943No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT5150.12573457065916654No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA5110.12475799146958078No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA5040.12304897788780571No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA4770.11645706835810181No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4700.11474805477632674No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG4640.1132831859919481No Hit
GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA4630.11303904119455166No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC4480.10937686923360507No Hit
GTACATGGGAATGGTATCAACGCAGAAAAAAAAAAAAAAAAAA4420.10791200044922643No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA4400.10742371085443354No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA4270.10424982848827984No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC4170.10180838051431543No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC4140.1010759461221261No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTGTT250.00549226629.5999987
TATAGTG250.00549226629.5999985
GATACAC1051.8189894E-1224.6666683
ATACACT901.4188117E-1024.6666664
TCAAATT551.8987846E-523.54545429
CAGTTAA400.001929076623.1251
AGGGCAA652.6758498E-622.7692310
TACACTG753.728619E-722.25
CAAATTC603.718752E-521.58333430
GCAGCGT806.9376256E-720.81251
CGAACTA450.003821667520.55555524
AGTGCTA450.003821667520.55555514
ACACTGC450.003821667520.5555556
CAGTCAA450.003821667520.55555515
AGCGAAC555.135329E-420.18181822
TAAGACT555.135329E-420.1818184
GCCGTCT1202.3646862E-1020.04166836
GTTTCTA656.888273E-519.92307716
GGTCGGT656.888273E-519.92307711
TTCAAAT656.888273E-519.92307728
Produced by FastQC (version 0.11.5)