##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630538.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	409593
Sequences flagged as poor quality	0
Sequence length	43
%GC	32
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.43537609285315	31.0	31.0	34.0	30.0	34.0
2	31.43590100416755	31.0	31.0	34.0	30.0	34.0
3	31.348931744438993	31.0	31.0	34.0	28.0	34.0
4	35.188062295986505	37.0	35.0	37.0	32.0	37.0
5	35.18362618501781	35.0	35.0	37.0	33.0	37.0
6	35.357015378680785	37.0	35.0	37.0	33.0	37.0
7	35.208172502948045	37.0	35.0	37.0	32.0	37.0
8	35.37520172463885	37.0	35.0	37.0	33.0	37.0
9	36.733503746401915	39.0	37.0	39.0	32.0	39.0
10	36.65811183296589	38.0	35.0	39.0	32.0	39.0
11	36.903474912901345	39.0	37.0	39.0	33.0	39.0
12	36.91067230152859	39.0	37.0	39.0	33.0	39.0
13	36.97901819611175	39.0	37.0	39.0	33.0	39.0
14	37.87478057486334	40.0	37.0	41.0	33.0	41.0
15	37.78284052705979	39.0	37.0	41.0	32.0	41.0
16	37.57622078502318	39.0	36.0	41.0	32.0	41.0
17	37.84717512262173	39.0	37.0	41.0	33.0	41.0
18	37.97628865727686	39.0	37.0	41.0	33.0	41.0
19	38.16761272775658	40.0	37.0	41.0	34.0	41.0
20	38.20388043740982	40.0	38.0	41.0	34.0	41.0
21	38.17830626988254	40.0	37.0	41.0	34.0	41.0
22	38.029138681569265	40.0	37.0	41.0	33.0	41.0
23	37.87928992927125	40.0	36.0	41.0	33.0	41.0
24	37.71923836589004	39.0	36.0	41.0	33.0	41.0
25	37.593447641927476	39.0	36.0	41.0	33.0	41.0
26	37.14671881599539	39.0	35.0	40.0	32.0	41.0
27	36.78597534625836	38.0	35.0	40.0	32.0	41.0
28	36.51016985153555	38.0	35.0	40.0	31.0	41.0
29	36.20782825878372	38.0	35.0	40.0	31.0	41.0
30	35.80448152190101	37.0	35.0	40.0	30.0	41.0
31	35.35771851569729	37.0	35.0	40.0	30.0	41.0
32	34.88451462793554	36.0	34.0	40.0	28.0	41.0
33	34.463098734597516	35.0	33.0	40.0	26.0	41.0
34	34.0997844201439	35.0	33.0	40.0	24.0	41.0
35	33.6902290810634	35.0	33.0	40.0	21.0	41.0
36	33.205054773885294	35.0	33.0	39.0	18.0	41.0
37	32.56732659005403	35.0	33.0	39.0	15.0	41.0
38	31.926026567836853	35.0	32.0	39.0	12.0	41.0
39	31.291833600671886	35.0	31.0	39.0	10.0	41.0
40	30.50936417370414	35.0	28.0	38.0	7.0	40.0
41	29.803673402621627	35.0	24.0	38.0	7.0	40.0
42	29.02943653822209	35.0	21.0	38.0	7.0	40.0
43	28.158794217674618	35.0	18.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	9.0
11	10.0
12	7.0
13	2.0
14	2.0
15	3.0
16	7.0
17	26.0
18	68.0
19	130.0
20	294.0
21	579.0
22	985.0
23	1497.0
24	2398.0
25	3701.0
26	5285.0
27	7370.0
28	10049.0
29	12799.0
30	15197.0
31	17484.0
32	20896.0
33	24811.0
34	29695.0
35	35745.0
36	51053.0
37	75325.0
38	66251.0
39	27910.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.46378722292617	20.78258173357455	13.931146284238254	12.822484759261021
2	16.015898709206454	28.213128642335196	32.05621189815256	23.714760750305793
3	26.430383331746395	31.917781798028777	28.877934925645704	12.773899944579131
4	14.055660130910441	18.166326084674296	28.560790833827728	39.217222950587534
5	9.475992021348022	48.52182532416325	27.640609092440542	14.36157356204818
6	29.060311089300843	28.508055557590094	19.966161531080854	22.465471822028206
7	21.627566877363627	39.699164780648104	23.36709855881326	15.306169783175005
8	35.07310915958036	28.19017903137993	20.876089190977385	15.86062261806232
9	29.715107435918092	10.639341980942058	26.02998586401623	33.61556471912362
10	21.039910350030397	34.261083563439804	25.84248265961577	18.856523426914034
11	23.67716245150674	27.656478504271316	20.131447558918243	28.534911485303706
12	19.30721472290786	35.984013398666484	30.529086190437827	14.179685687987831
13	28.69360560361139	21.26672086681169	34.517191455908666	15.52248207366825
14	22.733054519974708	24.97943080081935	36.69960179983545	15.587912879370498
15	27.06686881855891	23.701576931246386	34.88755911355907	14.343995136635638
16	16.899947020578963	25.566599038557786	39.23065091444434	18.30280302641891
17	14.927012912818336	31.751519191001798	36.82704538407639	16.49442251210348
18	12.875220035498652	23.954022651754304	42.84814437746739	20.322612935279654
19	14.361085272453385	28.49877805528903	45.90068677931507	11.239449892942506
20	15.632591377294045	23.86686295908377	47.42439445986626	13.076151203755924
21	18.946368712355923	24.889585515377462	43.71192867065599	12.452117101610623
22	17.334524759944628	26.147175366766522	42.78637574372609	13.731924129562762
23	15.456807123168609	27.368631788140913	43.00708264057247	14.16747844811801
24	12.861547926844452	30.024927183814178	43.637708652247476	13.475816237093897
25	12.818090152907887	29.352308266986988	43.682875439765816	14.146726140339313
26	13.892327261452223	29.152109533121905	43.372079112680154	13.583484092745726
27	12.546601138203044	31.039104672198985	43.019289880442294	13.395004309155675
28	12.572724631524466	30.82083922332657	44.465115370624005	12.141320774524955
29	12.069053914495608	31.83843473887493	44.28884282690378	11.803668519725678
30	11.433056717277882	33.29329358656032	43.90748865337054	11.366161042791258
31	11.205269621307004	33.638270185281485	42.79589739082455	12.360562802586958
32	10.01506373399936	34.71006584585186	43.23316072296157	12.041709697187208
33	10.452815355731177	34.67173511266062	42.953126640347854	11.922322891260348
34	10.778504515458028	35.488155315154316	40.85812013388901	12.875220035498652
35	10.487728061758867	35.807984999743645	40.690148513280256	13.014138425217228
36	10.686217782042174	35.897097850793344	39.16058135759156	14.256103009572918
37	10.765564841196017	36.220345562546235	39.313904290356525	13.700185305901226
38	11.280466218905108	35.134877793321664	39.131283981903984	14.453372005869241
39	11.582229188487107	35.70495589524235	38.9935863161724	13.719228600098146
40	11.30707800182132	35.963993525279975	38.56975094789218	14.15917752500653
41	10.919376063555774	36.6202547406816	37.40981901546169	15.050550180300934
42	11.095648607276003	36.324595391034514	37.289943919940036	15.289812081749442
43	10.850771375487374	36.828021963265975	36.13220929068612	16.188997370560532
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	32.0
1	127.5
2	223.0
3	939.5
4	1656.0
5	1656.0
6	2856.5
7	4057.0
8	4617.5
9	5178.0
10	7525.5
11	9873.0
12	9873.0
13	17406.0
14	24939.0
15	33846.0
16	42753.0
17	38073.5
18	33394.0
19	33394.0
20	34575.5
21	35757.0
22	25731.0
23	15705.0
24	13986.0
25	12267.0
26	12267.0
27	12619.5
28	12972.0
29	13137.0
30	13302.0
31	13060.0
32	12818.0
33	12818.0
34	12322.0
35	11826.0
36	11520.0
37	11214.0
38	12323.0
39	13432.0
40	13432.0
41	14512.5
42	15593.0
43	16597.0
44	17601.0
45	19850.5
46	22100.0
47	22100.0
48	23235.5
49	24371.0
50	20624.5
51	16878.0
52	13644.0
53	10410.0
54	10410.0
55	8732.5
56	7055.0
57	7082.0
58	7109.0
59	6616.0
60	6123.0
61	6123.0
62	5443.0
63	4763.0
64	4432.0
65	4101.0
66	3886.5
67	3672.0
68	3672.0
69	3285.0
70	2898.0
71	2546.0
72	2194.0
73	1831.5
74	1469.0
75	1469.0
76	1198.0
77	927.0
78	701.5
79	476.0
80	372.0
81	268.0
82	268.0
83	186.0
84	104.0
85	79.0
86	54.0
87	39.5
88	25.0
89	25.0
90	13.5
91	2.0
92	1.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	409593.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.277894509845424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.03278264335279	29.034208460649403
2	9.767729564564654	7.087053254879497
3	3.348013988545407	3.643766948818113
4	1.6055483759738407	2.3298365845613054
5	1.0235309027390243	1.856577305856659
6	0.6871306374427926	1.4956591667779484
7	0.4769828713320043	1.2112754002429937
8	0.37872521976037643	1.099148285654797
9	0.2875022882391731	0.9386979915671905
>10	1.9993421612030282	14.224797713048762
>50	0.22469566752580392	5.577280802406376
>100	0.14439901756913523	10.456667136428772
>500	0.01349523528683507	3.247130874469829
>1k	0.007422379407759288	4.600326462413229
>5k	6.747617643417535E-4	2.313993830106543
>10k+	0.0020242852930252605	10.883579782118586
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16705	4.078438840507529	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15504	3.7852209388344042	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12252	2.9912620577011815	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9453	2.3079007697885463	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2888	0.7050901748809184	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2524	0.6162214686286143	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2477	0.6047466631509816	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1741	0.4250560922672018	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1676	0.40918668043643325	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1426	0.34815048108732327	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1411	0.3444883091263767	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	1344	0.3281306077008152	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1258	0.30713415512472136	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	1037	0.25317815490010814	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1011	0.24683039016780073	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	954	0.23291413671620362	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	875	0.21362669772188486	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	811	0.19800143068851275	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	783	0.19116537636141243	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	749	0.18286445324993347	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	715	0.17456353013845452	No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA	710	0.17334280615147232	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT	709	0.17309866135407587	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	690	0.1684599102035435	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	655	0.15991484229466812	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	646	0.15771753911810016	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	634	0.1547878015493429	No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT	621	0.15161391918318917	TruSeq Adapter, Index 7 (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	617	0.1506373399936034	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	528	0.12890845302532025	No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA	521	0.12719943944354517	No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA	517	0.12622286025395943	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT	515	0.12573457065916654	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	511	0.12475799146958078	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	504	0.12304897788780571	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	477	0.11645706835810181	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	470	0.11474805477632674	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	464	0.1132831859919481	No Hit
GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	463	0.11303904119455166	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	448	0.10937686923360507	No Hit
GTACATGGGAATGGTATCAACGCAGAAAAAAAAAAAAAAAAAA	442	0.10791200044922643	No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA	440	0.10742371085443354	No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	427	0.10424982848827984	No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC	417	0.10180838051431543	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC	414	0.1010759461221261	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0017090135817750792	0.0
3	0.0	0.0	0.0	0.0026855927713608386	0.0
4	0.0	0.0	0.0	0.004882895947928798	0.0
5	0.0	0.0	0.0	0.0051270407453252375	0.0
6	0.0	0.0	0.0	0.005615330340118118	0.0
7	0.0	0.0	0.0	0.005615330340118118	0.0
8	0.0	0.0	0.0	0.006103619934910997	0.0
9	0.0	0.0	0.0	0.008545067908875395	0.0
10	0.0	2.441447973964399E-4	0.0	0.013183819059407754	0.0
11	0.0	2.441447973964399E-4	0.0	0.01831085980473299	0.0
12	0.0	4.882895947928798E-4	0.0	0.02636763811881551	0.0
13	0.0	4.882895947928798E-4	0.0	0.03295954764851938	0.0
14	0.0	4.882895947928798E-4	0.0	0.04174876035479122	0.0
15	0.0	4.882895947928798E-4	0.0	0.06201277853869573	0.0
16	0.0	4.882895947928798E-4	0.0	0.10205252531171187	0.0
17	0.0	4.882895947928798E-4	0.0	0.160403131889461	0.0
18	0.0	4.882895947928798E-4	0.0	0.1831085980473299	0.0
19	0.0	4.882895947928798E-4	0.0	0.22363663441513892	0.0
20	0.0	4.882895947928798E-4	0.0	0.24927183814176512	0.0
21	0.0	4.882895947928798E-4	0.0	0.31250534066744307	0.0
22	0.0	4.882895947928798E-4	0.0	0.42066148591406594	0.0
23	0.0	7.324343921893197E-4	0.0	0.5759375770582017	0.0
24	0.0	9.765791895857596E-4	0.0	0.8291157319583098	0.0
25	0.0	9.765791895857596E-4	0.0	0.8881987729282483	0.0
26	0.0	9.765791895857596E-4	0.0	0.9858566918868242	0.0
27	0.0	9.765791895857596E-4	0.0	1.0537289455630345	0.0
28	0.0	9.765791895857596E-4	0.0	1.1355174526908418	0.0
29	0.0	9.765791895857596E-4	0.0	1.252706955441133	0.0
30	0.0	9.765791895857596E-4	0.0	1.4631597707968642	0.0
31	0.0	9.765791895857596E-4	0.0	2.0268901079852437	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCTGTT	25	0.005492266	29.599998	7
TATAGTG	25	0.005492266	29.599998	5
GATACAC	105	1.8189894E-12	24.666668	3
ATACACT	90	1.4188117E-10	24.666666	4
TCAAATT	55	1.8987846E-5	23.545454	29
CAGTTAA	40	0.0019290766	23.125	1
AGGGCAA	65	2.6758498E-6	22.76923	10
TACACTG	75	3.728619E-7	22.2	5
CAAATTC	60	3.718752E-5	21.583334	30
GCAGCGT	80	6.9376256E-7	20.8125	1
CGAACTA	45	0.0038216675	20.555555	24
AGTGCTA	45	0.0038216675	20.555555	14
ACACTGC	45	0.0038216675	20.555555	6
CAGTCAA	45	0.0038216675	20.555555	15
AGCGAAC	55	5.135329E-4	20.181818	22
TAAGACT	55	5.135329E-4	20.181818	4
GCCGTCT	120	2.3646862E-10	20.041668	36
GTTTCTA	65	6.888273E-5	19.923077	16
GGTCGGT	65	6.888273E-5	19.923077	11
TTCAAAT	65	6.888273E-5	19.923077	28
>>END_MODULE