FastQCFastQC Report
Fri 10 Feb 2017
ERR1630537.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630537.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047356
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT76350.3729200002344487No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT67810.33120766491025494No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT61390.29985014819113043No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41050.20050250176324977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG1800.017.4722235
GTCTAAC752.0682614E-417.2666661
GGTATCA37100.016.7547191
CTAGCGG2650.016.75471729
TCTAGCG2500.016.27999928
GTGTAAT1504.6766218E-916.0333331
TAGAACA4300.015.9186044
CGAACTA1659.749783E-1015.6969724
GTCCTAG1557.21775E-915.51612951
GTATTAT1802.0190782E-1015.4166671
CTTATAC13600.014.82720537
GAACCGT1001.09402645E-414.86
TCTTTCG1702.4396286E-814.147058510
TAGATTG2009.840733E-1013.8750015
CTATACG800.006300837713.8754
GTACGAA1203.302836E-513.87499928
GTAGAAC4200.013.6547613
ACCGTGA950.001245655413.6315798
CTATACA1501.3068093E-613.5666664
AGACCGT2051.4260877E-913.5365866
Produced by FastQC (version 0.11.5)