##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630537.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2047356
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.072084678971315	33.0	31.0	34.0	30.0	34.0
2	32.25031455203687	34.0	31.0	34.0	30.0	34.0
3	32.34875859401101	34.0	31.0	34.0	30.0	34.0
4	35.92303243793459	37.0	35.0	37.0	35.0	37.0
5	35.87473746627357	37.0	35.0	37.0	35.0	37.0
6	35.94302602966948	37.0	35.0	37.0	35.0	37.0
7	35.89892622484805	37.0	35.0	37.0	35.0	37.0
8	35.8960669272955	37.0	35.0	37.0	35.0	37.0
9	37.60802176074898	39.0	37.0	39.0	35.0	39.0
10	37.51891756978269	39.0	37.0	39.0	35.0	39.0
11	37.613936706659715	39.0	37.0	39.0	35.0	39.0
12	37.56502484179595	39.0	37.0	39.0	35.0	39.0
13	37.596661254808645	39.0	37.0	39.0	35.0	39.0
14	38.912561371837626	40.0	38.0	41.0	36.0	41.0
15	38.91784916741397	40.0	38.0	41.0	36.0	41.0
16	38.87085831677539	40.0	38.0	41.0	35.0	41.0
17	38.857311576491824	40.0	38.0	41.0	35.0	41.0
18	38.83718610735016	40.0	38.0	41.0	35.0	41.0
19	38.9041920408566	40.0	38.0	41.0	35.0	41.0
20	38.86502396261324	40.0	38.0	41.0	35.0	41.0
21	38.82337951973179	40.0	38.0	41.0	35.0	41.0
22	38.759357923096914	40.0	38.0	41.0	35.0	41.0
23	38.70199613550355	40.0	38.0	41.0	34.0	41.0
24	38.65120135433212	40.0	38.0	41.0	34.0	41.0
25	38.60518835024295	40.0	38.0	41.0	34.0	41.0
26	38.46176483230078	40.0	38.0	41.0	34.0	41.0
27	38.33918722488907	40.0	38.0	41.0	34.0	41.0
28	38.243388057572794	40.0	38.0	41.0	34.0	41.0
29	38.1764500165091	40.0	38.0	41.0	33.0	41.0
30	38.087107957775785	40.0	38.0	41.0	33.0	41.0
31	38.04061579910871	40.0	37.0	41.0	33.0	41.0
32	37.941293551292496	40.0	37.0	41.0	33.0	41.0
33	37.868976377337404	40.0	37.0	41.0	33.0	41.0
34	37.802499418762544	40.0	37.0	41.0	33.0	41.0
35	37.73343863988481	40.0	37.0	41.0	33.0	41.0
36	37.70151698092564	40.0	37.0	41.0	33.0	41.0
37	37.567069430035616	40.0	37.0	41.0	33.0	41.0
38	37.41890369823324	40.0	37.0	41.0	32.0	41.0
39	37.359123181312874	40.0	36.0	41.0	32.0	41.0
40	37.18661581083114	40.0	36.0	41.0	31.0	41.0
41	37.11893388350634	40.0	36.0	41.0	31.0	41.0
42	36.990776884918894	40.0	36.0	41.0	31.0	41.0
43	36.19485961405832	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	8.0
11	9.0
12	3.0
13	2.0
14	4.0
15	4.0
16	7.0
17	29.0
18	66.0
19	157.0
20	293.0
21	513.0
22	991.0
23	1793.0
24	2942.0
25	4600.0
26	7002.0
27	10177.0
28	15042.0
29	20866.0
30	28223.0
31	36639.0
32	47323.0
33	61080.0
34	81177.0
35	109617.0
36	155167.0
37	243209.0
38	456962.0
39	763441.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.710033819228315	18.79448420304041	12.087980790834617	25.40750118689666
2	18.56936458534813	21.24657362959837	34.69000994453334	25.494051840520164
3	20.466201285951247	23.5454410468917	29.125125283536423	26.863232383620634
4	14.7268965436397	15.545513335247998	33.9722549473565	35.75533517375581
5	14.349531786362506	37.02355623545685	34.106086093478616	14.520825884702028
6	34.77040631917458	34.11942036460684	15.968986341408137	15.141186974810438
7	29.076623703938154	30.441115272575946	21.556729752910584	18.92553127057532
8	27.175781837648167	33.21537631950672	20.446321987968872	19.16251985487624
9	26.956035003194362	13.932750337508473	19.84803815262221	39.26317650667495
10	17.163453742290056	26.722905054128347	32.90541557013045	23.208225633451143
11	34.84679752812897	22.053419141566	21.49728723289941	21.60249609740563
12	21.604694054185007	25.50299019808963	29.15829000916304	23.734025738562323
13	29.643745396501636	19.99491050896864	26.044273687624433	24.317070406905298
14	23.262881492031674	21.02194244674595	25.37560639185369	30.33956966936869
15	25.654356154962795	27.16376634058757	22.92424961755552	24.25762788689412
16	25.247636463809908	26.379095770349657	24.43219449866071	23.941073267179718
17	23.51247169520103	26.692426720120977	25.841329011661866	23.953772573016124
18	23.213207668817734	25.33399174349747	27.407446482194597	24.0453541054902
19	24.151539839676147	25.425573275971548	27.283579406805657	23.13930747754665
20	24.525339022622347	24.69604699915403	27.284507433001394	23.49410654522223
21	24.488217974792857	25.103987777406566	26.394383780837334	24.013410466963244
22	24.476837443024078	25.063398842214056	26.385494266751852	24.074269448010018
23	23.84587731689066	25.340292552931682	26.754360257815446	24.059469872362207
24	23.75082789705357	25.84821594290392	26.635768278697014	23.7651878813455
25	24.29005996026094	25.220626017165554	26.562307678781806	23.927006343791703
26	23.83952766397246	26.131752367443667	26.542867972155303	23.485851996428565
27	24.60510043197177	25.22668260917984	25.90404404510012	24.26417291374827
28	23.601024931667965	25.532296288481337	26.911733963218904	23.954944816631794
29	23.209739781454715	25.730942737853113	27.12820828424563	23.93110919644654
30	23.20329244156854	26.44132236894805	27.145108129704848	23.210277059778566
31	23.85525526581601	26.043296817944707	26.331131469075235	23.77031644716405
32	22.961517195836972	25.853197978270508	26.842669276862452	24.342615549030068
33	23.06726333866704	25.783058735266362	26.899913840094246	24.24976408597235
34	23.94092673672776	25.44774821770127	26.878129646236417	23.733195399334555
35	23.711557735928682	25.679950140571545	27.35264409316211	23.255848030337663
36	23.1306621808811	25.838642620042627	26.944214880069712	24.08648031900656
37	23.74491783549124	25.03970975248076	27.105593751160033	24.109778660867967
38	23.235236080095497	24.955650116540554	27.631979978079045	24.177133825284905
39	23.392707472466927	25.19298060522938	27.30868495757455	24.105626964729144
40	22.71534603654665	25.149558747965667	28.073085481958195	24.06200973352949
41	22.132936333495493	25.025398611672813	28.663945107738957	24.177719947092736
42	22.86524668890022	24.935819662042167	28.1313557583537	24.067577890703912
43	22.236484519546185	24.63846053153433	28.402241720541028	24.722813228378453
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	367.0
1	354.0
2	341.0
3	827.5
4	1314.0
5	1314.0
6	1880.0
7	2446.0
8	2444.0
9	2442.0
10	3568.5
11	4695.0
12	4695.0
13	7839.0
14	10983.0
15	14816.0
16	18649.0
17	18536.5
18	18424.0
19	18424.0
20	21230.0
21	24036.0
22	23164.5
23	22293.0
24	24971.5
25	27650.0
26	27650.0
27	31146.5
28	34643.0
29	41248.0
30	47853.0
31	55378.0
32	62903.0
33	62903.0
34	68811.5
35	74720.0
36	82126.0
37	89532.0
38	98562.5
39	107593.0
40	107593.0
41	112595.5
42	117598.0
43	118350.0
44	119102.0
45	124650.5
46	130199.0
47	130199.0
48	132773.0
49	135347.0
50	141619.5
51	147892.0
52	154031.0
53	160170.0
54	160170.0
55	150512.0
56	140854.0
57	133716.0
58	126578.0
59	114074.0
60	101570.0
61	101570.0
62	95499.5
63	89429.0
64	79213.0
65	68997.0
66	60488.5
67	51980.0
68	51980.0
69	44354.5
70	36729.0
71	32273.5
72	27818.0
73	22609.0
74	17400.0
75	17400.0
76	13747.0
77	10094.0
78	8375.0
79	6656.0
80	5135.0
81	3614.0
82	3614.0
83	2724.5
84	1835.0
85	1537.5
86	1240.0
87	1069.0
88	898.0
89	898.0
90	652.0
91	406.0
92	228.5
93	51.0
94	29.5
95	8.0
96	8.0
97	7.0
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2047356.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.21127569250098
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.40022152096844	46.598438989023435
2	9.421533993845106	10.403498215609039
3	2.5207431542614294	4.175203357197369
4	1.0927019525501414	2.413178750079199
5	0.5926453719953969	1.6360353510561327
6	0.3640959541200776	1.2061321260868676
7	0.2487773275335325	0.9614719531548235
8	0.1690533763834368	0.7466922056203249
9	0.1277012322062293	0.634549314384919
>10	0.8331196258721464	8.982680209088688
>50	0.11203259190958953	4.3384998488501285
>100	0.10583319782729714	11.83803965326732
>500	0.008877461944202972	3.2267613348361737
>1k	0.0023969147249348023	1.8313441461071605
>5k	2.6632385832608914E-4	1.0074745456383867
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7635	0.3729200002344487	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6781	0.33120766491025494	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6139	0.29985014819113043	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4105	0.20050250176324977	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0012210870996543835	0.0
3	0.0	0.0	0.0	0.0021979567793778904	0.0
4	0.0	0.0	0.0	0.003760948266935501	0.0
5	0.0	0.0	0.0	0.004395913558755781	0.0
6	0.0	0.0	0.0	0.004444757042741956	0.0
7	0.0	0.0	0.0	0.005372783238479287	0.0
8	0.0	0.0	0.0	0.005861218078341041	0.0
9	0.0	0.0	0.0	0.00932910544135949	0.0
10	0.0	4.884348398617534E-5	0.0	0.013090053708294991	0.0
11	0.0	4.884348398617534E-5	0.0	0.014946106099769653	0.0
12	0.0	9.768696797235068E-5	0.0	0.016655628039285792	0.0
13	0.0	9.768696797235068E-5	0.0	0.017485967267050772	0.0
14	0.0	9.768696797235068E-5	0.0	0.018560523914746628	0.0
15	0.0	9.768696797235068E-5	0.0	0.020709637210138344	0.0
16	0.0	9.768696797235068E-5	0.0	0.022614533085599183	0.0
17	0.0	9.768696797235068E-5	0.0	0.026326637868548507	0.0
18	0.0	1.4653045195852602E-4	0.0	0.028671125099884925	0.0
19	0.0	1.4653045195852602E-4	0.0	0.03135751671912457	0.0
20	0.0	1.4653045195852602E-4	0.0	0.034678873630184494	0.0
21	0.0	1.4653045195852602E-4	0.0	0.03868403931705087	0.0
22	0.0	1.4653045195852602E-4	0.0	0.04513137920322601	0.0
23	0.0	1.4653045195852602E-4	0.0	0.054558171612557854	0.0
24	0.0	1.4653045195852602E-4	0.0	0.06847856454861782	0.0
25	0.0	1.4653045195852602E-4	0.0	0.07355828688318006	0.0
26	0.0	1.4653045195852602E-4	0.0	0.081617461740899	0.0
27	0.0	1.4653045195852602E-4	0.0	0.09798002887626774	0.0
28	0.0	1.4653045195852602E-4	0.0	0.13187740676267343	0.0
29	0.0	1.4653045195852602E-4	0.0	0.18096510816877964	0.0
30	0.0	1.4653045195852602E-4	0.0	0.25173931646474773	0.0
31	0.0	1.4653045195852602E-4	0.0	0.48975361392938016	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACAG	180	0.0	17.472223	5
GTCTAAC	75	2.0682614E-4	17.266666	1
GGTATCA	3710	0.0	16.754719	1
CTAGCGG	265	0.0	16.754717	29
TCTAGCG	250	0.0	16.279999	28
GTGTAAT	150	4.6766218E-9	16.033333	1
TAGAACA	430	0.0	15.918604	4
CGAACTA	165	9.749783E-10	15.69697	24
GTCCTAG	155	7.21775E-9	15.5161295	1
GTATTAT	180	2.0190782E-10	15.416667	1
CTTATAC	1360	0.0	14.827205	37
GAACCGT	100	1.09402645E-4	14.8	6
TCTTTCG	170	2.4396286E-8	14.1470585	10
TAGATTG	200	9.840733E-10	13.875001	5
CTATACG	80	0.0063008377	13.875	4
GTACGAA	120	3.302836E-5	13.874999	28
GTAGAAC	420	0.0	13.654761	3
ACCGTGA	95	0.0012456554	13.631579	8
CTATACA	150	1.3068093E-6	13.566666	4
AGACCGT	205	1.4260877E-9	13.536586	6
>>END_MODULE