FastQCFastQC Report
Fri 10 Feb 2017
ERR1630536.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630536.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424769
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35580.24972469221326404No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33910.23800349389971287No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27580.1935752392142165No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17150.12037038986670821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTA601.337783E-624.6666667
GGTATCA17800.017.9803371
CGCGCTT803.3832772E-416.187512
TTAACGG803.3832772E-416.187535
CGCGGTT2850.015.57894810
TTTCGGA2750.015.47272830
TCTTATA7650.015.23529437
CGAACGA1601.0968506E-815.0312516
ACGAACG1601.0968506E-815.0312515
TTCGGAA2850.014.92982631
GCTATAC750.004105048314.83
TTACACC1001.09365545E-414.84
ATAGGAC4350.014.459773
CGTGCAC908.275729E-414.38888910
AAACCGT908.275729E-414.3888896
TCTAAGC1458.913503E-714.0344843
TAAGACT3300.014.0151514
AGAACCG1753.5668563E-813.7428585
ATAACGA1753.5668563E-813.74285812
TAACGGC950.001245329513.63157936
Produced by FastQC (version 0.11.5)