##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630536.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1424769
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.02140206587875	31.0	31.0	33.0	28.0	34.0
2	31.218838983722975	31.0	31.0	34.0	28.0	34.0
3	31.306185774676457	31.0	31.0	34.0	28.0	34.0
4	35.120961362859525	37.0	35.0	37.0	32.0	37.0
5	34.63702537042847	35.0	35.0	37.0	31.0	37.0
6	34.75801761548714	35.0	35.0	37.0	32.0	37.0
7	34.7279580058241	35.0	35.0	37.0	32.0	37.0
8	34.71026110197513	35.0	35.0	37.0	32.0	37.0
9	36.26483170254266	37.0	35.0	39.0	32.0	39.0
10	35.956265892927206	37.0	35.0	39.0	31.0	39.0
11	36.17173240012942	37.0	35.0	39.0	32.0	39.0
12	36.04432227259296	37.0	35.0	39.0	32.0	39.0
13	36.16476635861673	37.0	35.0	39.0	32.0	39.0
14	37.157032473334276	38.0	36.0	40.0	32.0	41.0
15	37.14478347016253	38.0	36.0	40.0	32.0	41.0
16	37.07406604158288	38.0	36.0	40.0	32.0	41.0
17	37.00029689023273	38.0	36.0	40.0	32.0	41.0
18	36.96433807866398	38.0	36.0	40.0	31.0	41.0
19	37.00960436393549	39.0	36.0	40.0	31.0	41.0
20	36.95728219802649	39.0	36.0	40.0	31.0	41.0
21	36.90305095071552	38.0	36.0	40.0	31.0	41.0
22	36.833282447891555	38.0	36.0	40.0	31.0	41.0
23	36.748185846267006	38.0	36.0	40.0	31.0	41.0
24	36.65145507798106	38.0	35.0	40.0	31.0	41.0
25	36.58208172693258	38.0	35.0	40.0	30.0	41.0
26	36.37957451348254	38.0	35.0	40.0	30.0	41.0
27	36.22540636411938	38.0	35.0	40.0	30.0	41.0
28	36.08512467635104	38.0	35.0	40.0	29.0	41.0
29	35.97176945876841	38.0	34.0	40.0	29.0	41.0
30	35.85995835114324	38.0	34.0	40.0	29.0	41.0
31	35.815273212710274	38.0	34.0	40.0	29.0	41.0
32	35.6925389308723	38.0	34.0	40.0	28.0	41.0
33	35.61037403256247	38.0	34.0	40.0	28.0	41.0
34	35.56137731800734	38.0	34.0	40.0	28.0	41.0
35	35.479741628292025	38.0	34.0	40.0	27.0	41.0
36	35.447021938293155	38.0	34.0	40.0	27.0	41.0
37	35.296296452267	38.0	34.0	40.0	27.0	41.0
38	35.11562014614299	38.0	33.0	40.0	27.0	41.0
39	35.05816381462539	38.0	33.0	40.0	26.0	41.0
40	34.85389350835118	38.0	33.0	40.0	26.0	41.0
41	34.78666717201175	38.0	33.0	40.0	26.0	41.0
42	34.64204372779026	37.0	33.0	40.0	25.0	41.0
43	33.723604317612185	37.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	3.0
11	4.0
12	6.0
13	4.0
14	11.0
15	33.0
16	45.0
17	117.0
18	218.0
19	384.0
20	726.0
21	1344.0
22	2046.0
23	3542.0
24	5285.0
25	8025.0
26	11980.0
27	16726.0
28	23003.0
29	30571.0
30	40136.0
31	51403.0
32	66636.0
33	85874.0
34	114671.0
35	155297.0
36	211100.0
37	263393.0
38	242977.0
39	89202.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.97938823767221	19.968640530500032	12.377304671845051	24.674666559982704
2	19.640587351353098	21.812658753805003	33.70244579998582	24.84430809485608
3	21.051763478851658	24.00066256354539	29.019651606681506	25.927922350921445
4	15.48587876350482	15.779189468608596	34.35637636697598	34.37855540091061
5	14.339377120080519	37.37525170746977	34.37420381830318	13.911167354146531
6	35.15643588539616	35.17124530362466	15.397934682745063	14.274384128234122
7	29.405608909233706	30.472378329399362	21.455758793179807	18.666253968187124
8	27.673398284213093	33.31290896980493	20.0176309282417	18.99606181774028
9	27.118080194052506	13.698009993198898	19.53607918195862	39.647830630789976
10	17.00984510471522	26.976302825229915	32.75653807740061	23.257313992654247
11	36.0736372001356	21.84410244748447	21.289205478221383	20.79305487415855
12	22.292385642865614	25.56870622536004	28.737711165810037	23.401196965964306
13	30.361693720174987	19.835917260973535	25.358988018408596	24.44340100044288
14	23.237030002758342	20.668332901684412	25.132144228292447	30.9624928672648
15	25.8834239094197	27.748568364415565	22.24851888271011	24.119488843454622
16	25.498659782743726	26.505700222281646	23.730724068252467	24.264915926722157
17	24.045722499577124	26.397893272523476	25.174466878490477	24.381917349408923
18	23.669450977667257	25.044480894797683	26.975530770251176	24.31053735728388
19	24.82472597312266	25.179450142444143	26.634071909200717	23.361751975232476
20	25.413733735082673	24.440733901425425	26.18192843892589	23.96360392456602
21	25.344950655158836	25.077960006148363	25.596921325492062	23.980168013200736
22	25.21138514383735	25.030022410650428	25.767264728527923	23.991327716984298
23	24.362826535389246	25.139794591263566	26.08471969842129	24.4126591749259
24	24.446910341255318	25.597272259573305	25.966595286674544	23.989222112496833
25	24.799248158824344	25.111228557050303	25.801586081673587	24.287937202451765
26	24.565526060715808	26.003232804756422	25.679320647768165	23.75192048675961
27	25.169202867271817	24.713620243000793	25.45682844025944	24.660348449467946
28	24.22792747455903	25.263814695575213	26.216951660234045	24.29130616963171
29	24.016735344466365	25.508836871099806	26.39761252525848	24.076815259175348
30	24.075060588769126	26.011163914992537	26.09700239126483	23.816773104973507
31	24.380373239451448	26.006180651038868	25.737294957989683	23.876151151520002
32	23.615266755523177	25.58962189660219	25.87823008501729	24.916881262857345
33	23.6138630191982	25.618398491264198	26.33219841251459	24.435540077023013
34	24.884665514199146	24.826270083080136	26.095598654939856	24.193465747780866
35	24.481863375747224	25.13298647008743	26.411158580794503	23.973991573370842
36	23.76771252041559	25.917534702116622	26.133710096162954	24.181042681304827
37	24.695441857592353	25.02335466310679	26.256817771863368	24.02438570743749
38	24.19697508859331	25.088347654953193	26.489978375441915	24.224698881011587
39	24.33741890790718	25.04813060924262	26.265801684343216	24.348648798506986
40	23.644113537001438	25.013668882464458	26.882322678272757	24.459894902261347
41	23.014046487535875	25.138741789019836	27.384439161716738	24.46277256172755
42	23.89678607549715	24.957168495384163	27.212200714642165	23.93384471447652
43	23.712615869660276	24.433574846168046	27.13969773345714	24.714111550714538
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	110.0
1	110.0
2	110.0
3	293.5
4	477.0
5	477.0
6	764.0
7	1051.0
8	1087.0
9	1123.0
10	1542.0
11	1961.0
12	1961.0
13	3308.0
14	4655.0
15	6749.5
16	8844.0
17	8986.0
18	9128.0
19	9128.0
20	10875.0
21	12622.0
22	12894.5
23	13167.0
24	15394.0
25	17621.0
26	17621.0
27	20527.5
28	23434.0
29	28502.5
30	33571.0
31	38478.0
32	43385.0
33	43385.0
34	48309.0
35	53233.0
36	58383.5
37	63534.0
38	70041.0
39	76548.0
40	76548.0
41	79621.5
42	82695.0
43	82777.5
44	82860.0
45	85965.5
46	89071.0
47	89071.0
48	92710.5
49	96350.0
50	98101.5
51	99853.0
52	104539.5
53	109226.0
54	109226.0
55	102751.5
56	96277.0
57	92370.5
58	88464.0
59	83896.5
60	79329.0
61	79329.0
62	73931.0
63	68533.0
64	61567.5
65	54602.0
66	47148.0
67	39694.0
68	39694.0
69	34019.0
70	28344.0
71	24383.0
72	20422.0
73	15822.0
74	11222.0
75	11222.0
76	8329.0
77	5436.0
78	4415.0
79	3394.0
80	2616.5
81	1839.0
82	1839.0
83	1399.5
84	960.0
85	837.5
86	715.0
87	621.5
88	528.0
89	528.0
90	412.0
91	296.0
92	179.0
93	62.0
94	39.5
95	17.0
96	17.0
97	10.0
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1424769.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.777155762527364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.25781502851628	52.75801156219947
2	7.236429672717126	8.651463674211733
3	1.6900033191819548	3.030707749497839
4	0.7211404722116774	1.7243090533623995
5	0.42392077975872317	1.2670389241304627
6	0.2660566031888281	0.9542464206280509
7	0.18033786725696038	0.7546059346630913
8	0.129224356465586	0.6179731587804556
9	0.10130119149651144	0.544994739271492
>10	0.7533654760025468	9.228172143466454
>50	0.1298185030580248	5.450471112763586
>100	0.10456762521936842	12.01899580423506
>500	0.005074931604670385	1.8883411569360697
>1k	9.441733217991416E-4	1.1106685658538649
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3558	0.24972469221326404	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3391	0.23800349389971287	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2758	0.1935752392142165	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1715	0.12037038986670821	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.018681624880945E-5	0.0
2	0.0	0.0	0.0	4.2112089749285676E-4	0.0
3	0.0	0.0	0.0	7.72054978736904E-4	0.0
4	0.0	0.0	0.0	0.001544109957473808	0.0
5	0.0	0.0	0.0	0.0017546704062202363	0.0
6	0.0	0.0	0.0	0.0017546704062202363	0.0
7	0.0	0.0	0.0	0.001965230854966665	0.0
8	0.0	0.0	0.0	0.002105604487464284	0.0
9	0.0	0.0	0.0	0.0034391539961916634	0.0
10	0.0	0.0	0.0	0.005334198034909519	0.0
11	0.0	0.0	0.0	0.006316813462392851	0.0
12	0.0	0.0	0.0	0.006808121176134517	0.0
13	0.0	0.0	0.0	0.007018681624880945	0.0
14	0.0	0.0	0.0	0.008001297052364279	0.0
15	0.0	0.0	0.0	0.008773352031101182	0.0
16	0.0	0.0	0.0	0.009966527907330942	0.0
17	0.0	0.0	0.0	0.011721198313551178	0.0
18	0.0	0.0	0.0	0.01277400055728332	0.0
19	0.0	0.0	0.0	0.01417773688225951	0.0
20	0.0	0.0	0.0	0.01572184683973332	0.0
21	0.0	0.0	0.0	0.018669693122183317	0.0
22	0.0	0.0	0.0	0.02351258344335117	0.0
23	0.0	0.0	0.0	0.030180330986988066	0.0
24	0.0	0.0	0.0	0.03733938624436663	0.0
25	0.0	0.0	0.0	0.03993629844557258	0.0
26	0.0	0.0	0.0	0.04407732060425234	0.0
27	0.0	0.0	0.0	0.04920095819041543	0.0
28	0.0	0.0	0.0	0.06253645327768922	0.0
29	0.0	0.0	0.0	0.08471548721231302	0.0
30	7.018681624880945E-5	0.0	0.0	0.11826478537924394	0.0
31	7.018681624880945E-5	0.0	0.0	0.24389918646461287	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTA	60	1.337783E-6	24.666666	7
GGTATCA	1780	0.0	17.980337	1
CGCGCTT	80	3.3832772E-4	16.1875	12
TTAACGG	80	3.3832772E-4	16.1875	35
CGCGGTT	285	0.0	15.578948	10
TTTCGGA	275	0.0	15.472728	30
TCTTATA	765	0.0	15.235294	37
CGAACGA	160	1.0968506E-8	15.03125	16
ACGAACG	160	1.0968506E-8	15.03125	15
TTCGGAA	285	0.0	14.929826	31
GCTATAC	75	0.0041050483	14.8	3
TTACACC	100	1.09365545E-4	14.8	4
ATAGGAC	435	0.0	14.45977	3
CGTGCAC	90	8.275729E-4	14.388889	10
AAACCGT	90	8.275729E-4	14.388889	6
TCTAAGC	145	8.913503E-7	14.034484	3
TAAGACT	330	0.0	14.015151	4
AGAACCG	175	3.5668563E-8	13.742858	5
ATAACGA	175	3.5668563E-8	13.742858	12
TAACGGC	95	0.0012453295	13.631579	36
>>END_MODULE