FastQCFastQC Report
Fri 10 Feb 2017
ERR1630535.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630535.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480777
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC4940.1027503395545128No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGTT250.005493202729.611
CTTTGCG250.005493202729.69
CGAACGT400.00192956223.1250024
TATTAGA753.731402E-722.22
GGTATCA3350.021.5373131
CCGTCGT450.003822620320.5555559
GATATAC450.003822620320.5555553
CGTCGTA450.003822620320.55555510
ACCGTCG450.003822620320.5555558
TAAACAT555.137116E-420.1818185
TAGGACA555.137116E-420.1818184
ATTAGAG1355.638867E-1119.1851863
GACAGTT500.007029075618.57
ACCGCCT801.6134845E-518.512
CGACCCA801.6134845E-518.536
GCGTTAT609.226162E-418.51
CGTTATT500.007029075618.52
TCAATAG500.007029075618.53
ATATAGT500.007029075618.54
TTAACGG500.007029075618.535
Produced by FastQC (version 0.11.5)