##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630535.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	480777
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.483500666629226	31.0	30.0	31.0	27.0	34.0
2	30.703794066687884	31.0	31.0	33.0	27.0	34.0
3	30.797667109699507	31.0	31.0	34.0	27.0	34.0
4	34.626614833904284	35.0	35.0	37.0	32.0	37.0
5	33.73961940775037	35.0	33.0	37.0	28.0	37.0
6	33.90345420018013	35.0	33.0	37.0	29.0	37.0
7	33.89409851136806	35.0	33.0	37.0	29.0	37.0
8	33.871576635321574	35.0	33.0	37.0	29.0	37.0
9	35.36550001351978	37.0	34.0	39.0	30.0	39.0
10	34.930487523321624	37.0	33.0	39.0	29.0	39.0
11	35.23471172705849	37.0	34.0	39.0	30.0	39.0
12	35.03370585531338	37.0	34.0	39.0	29.0	39.0
13	35.213970302239915	37.0	34.0	39.0	30.0	39.0
14	36.06979535210711	38.0	34.0	40.0	30.0	41.0
15	36.03383897316219	38.0	34.0	40.0	30.0	41.0
16	35.965351919912976	38.0	34.0	40.0	30.0	41.0
17	35.8485430875437	38.0	34.0	40.0	29.0	41.0
18	35.78619401510472	38.0	34.0	40.0	29.0	41.0
19	35.800701780659224	38.0	34.0	40.0	29.0	41.0
20	35.75138161767306	38.0	34.0	40.0	29.0	41.0
21	35.66924582498747	38.0	34.0	40.0	29.0	40.0
22	35.57761706570822	38.0	34.0	40.0	28.0	40.0
23	35.50483280190192	38.0	34.0	40.0	27.0	40.0
24	35.371866790632666	38.0	34.0	39.0	27.0	40.0
25	35.276469132258825	38.0	33.0	39.0	27.0	40.0
26	35.02854129877261	37.0	33.0	39.0	27.0	40.0
27	34.85638248085911	37.0	33.0	39.0	26.0	40.0
28	34.70154770298912	37.0	33.0	39.0	26.0	40.0
29	34.55126181160912	37.0	33.0	39.0	25.0	40.0
30	34.430003931136476	37.0	33.0	39.0	25.0	40.0
31	34.382942611647394	37.0	32.0	39.0	25.0	40.0
32	34.27277927188697	37.0	32.0	39.0	25.0	40.0
33	34.17235225478756	37.0	32.0	39.0	25.0	40.0
34	34.124976860373934	37.0	32.0	39.0	24.0	40.0
35	34.041846843755	37.0	32.0	39.0	24.0	40.0
36	34.038227702240334	37.0	32.0	39.0	24.0	40.0
37	33.864560908695715	36.0	31.0	39.0	24.0	40.0
38	33.67534844636911	36.0	31.0	39.0	24.0	40.0
39	33.62310801057455	36.0	31.0	39.0	24.0	40.0
40	33.42104967583724	36.0	31.0	39.0	23.0	40.0
41	33.342245989304814	36.0	31.0	39.0	23.0	40.0
42	33.18160602524663	36.0	31.0	39.0	23.0	40.0
43	32.213521029500164	35.0	30.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	0.0
12	1.0
13	4.0
14	7.0
15	14.0
16	27.0
17	68.0
18	121.0
19	263.0
20	458.0
21	809.0
22	1320.0
23	2150.0
24	3159.0
25	4669.0
26	6520.0
27	9086.0
28	12352.0
29	16003.0
30	20795.0
31	26164.0
32	33358.0
33	42229.0
34	52839.0
35	62379.0
36	67551.0
37	60419.0
38	41172.0
39	16838.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.609567429390346	19.39194262620716	12.07482887076545	25.92366107363705
2	21.654114069516638	20.925917837167752	31.10007342281349	26.319894670502126
3	22.31138344804348	22.18221753536463	27.891101279803323	27.615297736788573
4	17.077772023204105	15.304808674291822	31.93663590396379	35.68078339854028
5	15.953134197351371	35.71364686746662	33.373892677894325	14.959326257287684
6	37.97623430405365	33.38013257705755	14.049132966011268	14.59450015287753
7	31.307030078393932	28.80087857780218	19.939597776099937	19.952493567703947
8	29.00783523338263	31.77564650555247	18.83971155026135	20.376806710803553
9	27.513171387150383	12.930319046044216	17.962797721188824	41.593711845616575
10	17.934302181676745	25.83921443829468	31.695983792901906	24.530499587126673
11	38.81758070789576	20.184617816576083	19.68916982301566	21.308631652512496
12	24.074778951572142	23.780463707706485	26.796830963211633	25.34792637750974
13	32.59452927240696	18.25690496841571	23.088250893865556	26.060314865311778
14	24.308567173554476	19.22180137568977	22.927261495454236	33.542369955301524
15	27.398773235824507	26.4950278403501	20.316071692281454	25.790127231543934
16	27.539170966997172	24.78196752340482	21.791807844385236	25.887053665212772
17	25.922621090443176	24.778639577184432	22.974060739178455	26.324678593193934
18	25.858558125700686	23.51048407057742	24.40653359041718	26.224424213304715
19	27.025627265863385	23.61469038660335	24.088922723008796	25.27075962452447
20	27.590546136774428	22.9825886013682	23.254856201523783	26.172009060333583
21	27.603233931739663	23.388806036894444	23.113626483796022	25.89433354756987
22	27.56766650650925	23.47158869912662	23.09511478294511	25.865630011419015
23	26.677232895916404	23.375286255374114	23.278567818344058	26.66891303036543
24	26.521235416835665	23.885502010287514	23.40794172766168	26.185320845215145
25	26.94305260026998	23.533779694120145	23.112794497240923	26.410373208368952
26	26.749823722848635	24.108682403692356	23.251944248580944	25.88954962487806
27	27.147721292823906	23.046651566110693	23.203273035107753	26.602354105957648
28	26.507923631954107	23.50403617477542	23.57121908909952	26.416821104170957
29	26.346518240265237	23.791487529561522	24.00863602044191	25.85335820973133
30	26.426388949554575	24.016955885992882	23.505700147885612	26.050955016566935
31	26.578226495859823	24.236392339899787	23.177273455260963	26.008107708979423
32	25.981276142577535	23.551043415138412	23.48781243695102	26.979868005333035
33	25.607922175977638	23.905885680887398	23.834126840510258	26.65206530262471
34	27.177880805446186	22.960748954296896	23.739696366506717	26.1216738737502
35	26.36211798817331	23.17560948215077	24.373254128213286	26.08901840146263
36	25.673025123913995	24.07810689779253	24.0127959532174	26.236072025076073
37	26.441364707546327	22.891486073585053	24.54963527789391	26.117513940974714
38	26.00082782662232	23.02543590895571	24.510531909804335	26.46320435461763
39	25.849614270233396	22.94265324672353	24.674849254436047	26.532883228607023
40	25.24829598753684	22.686193391114802	25.504755843145578	26.56075477820278
41	24.55441920058572	22.824927149177267	25.945916713985902	26.674736936251108
42	25.172793207661766	22.545795659942137	26.278919332663587	26.002491799732518
43	25.008059869752504	22.051803642853134	26.02911536949563	26.911021117898738
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	10.0
2	10.0
3	19.0
4	28.0
5	28.0
6	50.5
7	73.0
8	77.5
9	82.0
10	109.5
11	137.0
12	137.0
13	244.5
14	352.0
15	568.0
16	784.0
17	810.0
18	836.0
19	836.0
20	1037.0
21	1238.0
22	1386.0
23	1534.0
24	1966.0
25	2398.0
26	2398.0
27	3051.0
28	3704.0
29	4649.0
30	5594.0
31	6969.0
32	8344.0
33	8344.0
34	10109.5
35	11875.0
36	13819.5
37	15764.0
38	18030.5
39	20297.0
40	20297.0
41	21880.5
42	23464.0
43	25056.0
44	26648.0
45	28151.0
46	29654.0
47	29654.0
48	31804.0
49	33954.0
50	35311.5
51	36669.0
52	37985.5
53	39302.0
54	39302.0
55	38539.5
56	37777.0
57	37315.0
58	36853.0
59	35606.5
60	34360.0
61	34360.0
62	32353.5
63	30347.0
64	27654.5
65	24962.0
66	21899.5
67	18837.0
68	18837.0
69	16436.5
70	14036.0
71	11960.0
72	9884.0
73	7396.5
74	4909.0
75	4909.0
76	3786.0
77	2663.0
78	2126.5
79	1590.0
80	1264.0
81	938.0
82	938.0
83	691.0
84	444.0
85	331.0
86	218.0
87	172.5
88	127.0
89	127.0
90	97.0
91	67.0
92	38.0
93	9.0
94	5.5
95	2.0
96	2.0
97	2.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	480777.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.9192661497545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.1391713634964	65.54662321959383
2	5.205337791703682	7.48728148081825
3	1.3318793683282493	2.873633603104988
4	0.5983953991688035	1.7214463190243907
5	0.3630613095105854	1.3055551473685094
6	0.2218981110040874	0.9575249582058437
7	0.16344596892063923	0.8228439897935569
8	0.12092850670612368	0.6957671567112065
9	0.09435560987325595	0.6107387597277388
>10	0.6413841423852771	9.330725314472833
>50	0.08386754172123781	4.126804366140698
>100	0.03627488718157371	4.521055685038236
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	494	0.1027503395545128	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.159932775486348E-4	0.0
2	0.0	0.0	0.0	0.0014559764714202218	0.0
3	0.0	0.0	0.0	0.0018719697489688566	0.0
4	0.0	0.0	0.0	0.004159932775486348	0.0
5	0.0	0.0	0.0	0.0047839226918093	0.0
6	0.0	0.0	0.0	0.0047839226918093	0.0
7	0.0	0.0	0.0	0.00561590924690657	0.0
8	0.0	0.0	0.0	0.006655892440778157	0.0
9	0.0	0.0	0.0	0.01123181849381314	0.0
10	0.0	0.0	0.0	0.015183754630525171	0.0
11	0.0	0.0	0.0	0.017887710934591298	0.0
12	0.0	0.0	0.0	0.018511700850914248	0.0
13	0.0	0.0	0.0	0.019343687406011518	0.0
14	0.0	0.0	0.0	0.020383670599883105	0.0
15	0.0	0.0	0.0	0.022047643710077645	0.0
16	0.0	0.0	0.0	0.02308762690394923	0.0
17	0.0	0.0	0.0	0.02475160001414377	0.0
18	0.0	0.0	0.0	0.02620757648556399	0.0
19	0.0	0.0	0.0	0.02787154959575853	0.0
20	0.0	0.0	0.0	0.030783502538598975	0.0
21	0.0	0.0	0.0	0.03369545548143942	0.0
22	0.0	0.0	0.0	0.03972735800589462	0.0
23	0.0	0.0	0.0	0.04430328405892961	0.0
24	0.0	0.0	0.0	0.05158316641603072	0.0
25	0.0	0.0	0.0	0.05511910927519411	0.0
26	0.0	0.0	0.0	0.058863048773131824	0.0
27	0.0	0.0	0.0	0.06676692104655589	0.0
28	0.0	0.0	0.0	0.08923055803418217	0.0
29	0.0	0.0	0.0	0.11980606393400682	0.0
30	0.0	0.0	0.0	0.15932542530112714	0.0
31	0.0	0.0	0.0	0.31137096824515315	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCGTT	25	0.0054932027	29.6	11
CTTTGCG	25	0.0054932027	29.6	9
CGAACGT	40	0.001929562	23.125002	4
TATTAGA	75	3.731402E-7	22.2	2
GGTATCA	335	0.0	21.537313	1
CCGTCGT	45	0.0038226203	20.555555	9
GATATAC	45	0.0038226203	20.555555	3
CGTCGTA	45	0.0038226203	20.555555	10
ACCGTCG	45	0.0038226203	20.555555	8
TAAACAT	55	5.137116E-4	20.181818	5
TAGGACA	55	5.137116E-4	20.181818	4
ATTAGAG	135	5.638867E-11	19.185186	3
GACAGTT	50	0.0070290756	18.5	7
ACCGCCT	80	1.6134845E-5	18.5	12
CGACCCA	80	1.6134845E-5	18.5	36
GCGTTAT	60	9.226162E-4	18.5	1
CGTTATT	50	0.0070290756	18.5	2
TCAATAG	50	0.0070290756	18.5	3
ATATAGT	50	0.0070290756	18.5	4
TTAACGG	50	0.0070290756	18.5	35
>>END_MODULE