FastQCFastQC Report
Fri 10 Feb 2017
ERR1630534.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630534.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374503
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT81792.1839611431684123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT72071.9244171608772158No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT62861.6784912270395698No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33390.8915816428706846No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9970.2662194962390154No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8140.2173547341409815No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA8020.2141504874460285No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT7690.20533880903490762No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7190.1919877811392699No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA6710.17917079435945774No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG6350.1695580542745986No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA5870.15674106749478642No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5870.15674106749478642No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5310.14178791625167222No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT5250.1401857929041957No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG4960.13244219672472582No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG4400.11748904548161163No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT4300.11481883990248411Illumina PCR Primer Index 11 (95% over 22bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4250.11348373711292031No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA4050.10814332595466525No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4010.10707524372301425No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG4010.10707524372301425No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3990.10654120260718872No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC3920.10467205870179945No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG3750.10013270921728265No Hit
GTCCTAAACTACTAAACCTGCATTAAAAATTTCGGTTGGGGCG3750.10013270921728265No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTGT200.00184035237.010
TTATGTG250.00549167129.634
CGTGTCT250.00549167129.612
CCCTGCG358.858187E-426.4285727
CGTATTA400.001928769923.12500215
CCTACAC400.001928769923.1250023
TCCGTAT400.001928769923.12500213
ACTCGCA400.001928769923.12500221
TACTCGC400.001928769923.12500220
CAGTCCT400.001928769923.12500214
TGACGCA502.697391E-422.210
TTAATCT603.717657E-521.5833344
GACACAT957.1067916E-921.42105326
CACATGT957.1067916E-921.42105328
GCCGTCT705.08678E-621.14285936
ATCCGTA450.00382106620.55555512
CCGTATT450.00382106620.55555514
TCTGTTG450.00382106620.5555558
ACATGTT909.435462E-820.55555529
AAGGCCA450.00382106620.55555525
Produced by FastQC (version 0.11.5)