##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630534.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	374503
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.92403799168498	33.0	31.0	34.0	30.0	34.0
2	32.03093166142861	33.0	31.0	34.0	30.0	34.0
3	32.069334558067624	33.0	31.0	34.0	30.0	34.0
4	35.72528925001936	37.0	35.0	37.0	33.0	37.0
5	35.730194417668216	37.0	35.0	37.0	35.0	37.0
6	35.85416672229595	37.0	35.0	37.0	35.0	37.0
7	35.78700571156973	37.0	35.0	37.0	35.0	37.0
8	35.83167825090854	37.0	35.0	37.0	35.0	37.0
9	37.45794826743711	39.0	37.0	39.0	35.0	39.0
10	37.375922222251894	39.0	37.0	39.0	34.0	39.0
11	37.49700803464859	39.0	37.0	39.0	35.0	39.0
12	37.46839945207382	39.0	37.0	39.0	35.0	39.0
13	37.5021107975103	39.0	37.0	39.0	35.0	39.0
14	38.627709257335724	40.0	38.0	41.0	34.0	41.0
15	38.578849835648846	40.0	38.0	41.0	34.0	41.0
16	38.48794535691303	40.0	38.0	41.0	34.0	41.0
17	38.5925827029423	40.0	38.0	41.0	34.0	41.0
18	38.68100389048953	40.0	38.0	41.0	35.0	41.0
19	38.805181800946855	40.0	38.0	41.0	35.0	41.0
20	38.78838086744298	40.0	38.0	41.0	35.0	41.0
21	38.75936908382577	40.0	38.0	41.0	35.0	41.0
22	38.637719858051874	40.0	38.0	41.0	34.0	41.0
23	38.55057502877146	40.0	38.0	41.0	34.0	41.0
24	38.48170241626903	40.0	38.0	41.0	34.0	41.0
25	38.39088605431732	40.0	38.0	41.0	34.0	41.0
26	38.177704851496514	40.0	37.0	41.0	34.0	41.0
27	37.97886265263563	40.0	37.0	41.0	33.0	41.0
28	37.828369866196	40.0	37.0	41.0	33.0	41.0
29	37.6802909455999	40.0	36.0	41.0	33.0	41.0
30	37.45534214679188	39.0	36.0	41.0	33.0	41.0
31	37.246604166054745	39.0	35.0	41.0	33.0	41.0
32	36.99732979442087	39.0	35.0	41.0	32.0	41.0
33	36.809366013089345	39.0	35.0	41.0	31.0	41.0
34	36.64520978470132	39.0	35.0	41.0	31.0	41.0
35	36.427521274862954	39.0	35.0	41.0	31.0	41.0
36	36.269797571715046	39.0	35.0	41.0	30.0	41.0
37	35.96929797625119	39.0	35.0	41.0	30.0	41.0
38	35.67397323919968	38.0	35.0	41.0	28.0	41.0
39	35.429681471176465	38.0	35.0	40.0	26.0	41.0
40	35.06057094335693	38.0	35.0	40.0	24.0	41.0
41	34.79444757451876	38.0	35.0	40.0	22.0	41.0
42	34.4489176321685	38.0	34.0	40.0	20.0	41.0
43	33.59808599664088	37.0	33.0	40.0	16.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	6.0
12	6.0
13	4.0
14	4.0
15	2.0
16	4.0
17	7.0
18	24.0
19	36.0
20	71.0
21	130.0
22	279.0
23	521.0
24	866.0
25	1379.0
26	2079.0
27	3059.0
28	4524.0
29	6163.0
30	7970.0
31	9445.0
32	11482.0
33	14381.0
34	18546.0
35	23737.0
36	33122.0
37	57253.0
38	81294.0
39	98102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.82898935389036	18.966470228542896	13.382002280355564	19.822538137211186
2	18.55472452824143	23.013166783710677	35.01867808802599	23.413430600021893
3	21.64335132161825	27.89857491128242	29.536211992961338	20.92186177413799
4	12.315255151494114	16.819624942924357	34.01120952302118	36.85391038256035
5	11.095505242948654	42.52996638211176	32.86702643236502	13.50750194257456
6	30.29455037743356	34.43069881950211	17.744851176092048	17.52989962697228
7	26.278294165867834	33.57436389027591	22.008101403727075	18.139240540129183
8	31.484393983492787	29.95730341278975	19.38782866892922	19.17047393478824
9	28.282016432445133	11.409788439611965	21.606769505184204	38.7014256227587
10	19.668200254737613	29.082010023951742	28.863587207579112	22.386202513731533
11	31.1559586972601	24.77256523979781	19.777411662923928	24.294064400018158
12	22.4022237472063	31.042207939589268	27.194975741182315	19.36059257202212
13	30.49935514535264	20.303709182569964	28.292697254761645	20.90423841731575
14	23.560825947989734	21.976325957335455	29.377601781561165	25.085246313113647
15	27.54931202153254	25.080172922513306	27.609658667620817	19.760856388333337
16	22.566708410880555	25.0676229562914	29.612312851966472	22.75335578086157
17	19.817464746610842	28.47133400800528	29.216588385139776	22.49461286024411
18	19.068205061107655	23.82197205362841	33.55140012229542	23.55842276296852
19	20.914385198516435	25.59845982542196	34.579963311375344	18.907191664686266
20	22.056699145267196	23.004088084741646	35.5735468073687	19.365665962622465
21	23.613161977340635	23.816364621912243	33.09613007105417	19.474343329692953
22	23.588062044896837	24.27804316654339	31.737796492951993	20.39609829560778
23	21.18781424981909	25.498594136762588	32.818161670266996	20.495429943151322
24	19.944566532177312	26.87775531838196	32.48385193176023	20.6938262176805
25	20.456444941696063	25.615282120570466	33.0611503779676	20.86712255976588
26	21.01665407219702	25.887376069083558	32.765291599800264	20.330678258919153
27	20.42413545418862	26.60806455489008	32.0624934913739	20.9053064995474
28	19.405719046309375	26.018750183576632	34.70492893247851	19.87060183763548
29	20.089558695123937	26.548518970475538	34.691043863467044	18.670878470933477
30	18.851117347524585	27.768268879020997	34.06995404576199	19.310659727692435
31	19.21239616238054	28.341294996301762	32.85954985674347	19.586758984574224
32	18.037238687006514	28.130615776108602	33.77783355540543	20.054311981479454
33	18.048720570996764	27.768268879020997	34.10813798554351	20.074872564438735
34	19.63188545886148	27.605386338694217	32.873434925754935	19.889293276689372
35	18.403056851346985	28.94663060108998	32.51722950149932	20.133083046063717
36	18.55098624043065	29.237683009214884	31.173582054082345	21.037748696272125
37	18.61667329767719	28.326608865616564	32.37971391417425	20.677003922531995
38	18.465005620782744	27.51273020509849	32.76128629143158	21.260977882687186
39	18.372883528302843	28.55411038095823	32.74232783181977	20.330678258919153
40	17.85967001599453	28.283084514676787	32.95033684643381	20.906908622894875
41	17.378231950077836	28.793093780290146	32.42617549125107	21.402498778380945
42	17.14886129083078	28.78081083462616	32.977305922782996	21.093021951760065
43	17.497323118906923	28.419799040328115	32.2467376763337	21.83614016443126
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	21.0
1	48.0
2	75.0
3	449.0
4	823.0
5	823.0
6	1340.5
7	1858.0
8	1910.5
9	1963.0
10	2630.0
11	3297.0
12	3297.0
13	5661.0
14	8025.0
15	12417.5
16	16810.0
17	14813.0
18	12816.0
19	12816.0
20	13125.5
21	13435.0
22	9341.0
23	5247.0
24	4898.5
25	4550.0
26	4550.0
27	4909.5
28	5269.0
29	6012.5
30	6756.0
31	7657.0
32	8558.0
33	8558.0
34	10342.5
35	12127.0
36	13011.0
37	13895.0
38	16795.5
39	19696.0
40	19696.0
41	21371.5
42	23047.0
43	25350.0
44	27653.0
45	28084.5
46	28516.0
47	28516.0
48	30456.5
49	32397.0
50	30409.5
51	28422.0
52	25320.5
53	22219.0
54	22219.0
55	19664.0
56	17109.0
57	15331.0
58	13553.0
59	12520.5
60	11488.0
61	11488.0
62	10545.0
63	9602.0
64	8595.5
65	7589.0
66	6872.5
67	6156.0
68	6156.0
69	5102.0
70	4048.0
71	3324.5
72	2601.0
73	2165.5
74	1730.0
75	1730.0
76	1450.5
77	1171.0
78	987.5
79	804.0
80	651.0
81	498.0
82	498.0
83	415.5
84	333.0
85	260.0
86	187.0
87	153.5
88	120.0
89	120.0
90	77.0
91	34.0
92	19.0
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	374503.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.52213338267916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.91416406830083	27.321551950514504
2	10.153482907593014	7.2134674828454415
3	3.806784826278474	4.056753550746691
4	1.925214812738361	2.73550949473607
5	1.2544687339887557	2.228070284657462
6	0.8681899096104796	1.850397466240777
7	0.6566124943505681	1.6326993623538182
8	0.5110279418597323	1.4522242170413935
9	0.41000634281467224	1.3107869997486536
>10	2.9551443323626336	20.821113301132822
>50	0.3549787133862719	8.776121806761132
>100	0.17861986214978015	11.860789005977194
>500	0.008290373348723972	2.0445758896383506
>1k	7.53670304429452E-4	0.8939163147181421
>5k	0.0022610109132883563	5.802022872887565
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8179	2.1839611431684123	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7207	1.9244171608772158	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6286	1.6784912270395698	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3339	0.8915816428706846	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	997	0.2662194962390154	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	814	0.2173547341409815	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	802	0.2141504874460285	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT	769	0.20533880903490762	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	719	0.1919877811392699	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	671	0.17917079435945774	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	635	0.1695580542745986	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	587	0.15674106749478642	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	587	0.15674106749478642	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	531	0.14178791625167222	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	525	0.1401857929041957	No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG	496	0.13244219672472582	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	440	0.11748904548161163	No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT	430	0.11481883990248411	Illumina PCR Primer Index 11 (95% over 22bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	425	0.11348373711292031	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	405	0.10814332595466525	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	401	0.10707524372301425	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	401	0.10707524372301425	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	399	0.10654120260718872	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	392	0.10467205870179945	No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG	375	0.10013270921728265	No Hit
GTCCTAAACTACTAAACCTGCATTAAAAATTTCGGTTGGGGCG	375	0.10013270921728265	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.670205579127537E-4	0.0
2	0.0	0.0	0.0	0.0034712672528657983	0.0
3	0.0	0.0	0.0	0.006408493389906089	0.0
4	0.0	0.0	0.0	0.010413801758597395	0.0
5	0.0	0.0	0.0	0.013351027895637685	0.0
6	0.0	0.0	0.0	0.013351027895637685	0.0
7	0.0	0.0	0.0	0.0144191101272887	0.0
8	0.0	0.0	0.0	0.016021233474765223	0.0
9	0.0	0.0	0.0	0.02670205579127537	0.0
10	0.0	0.0	0.0	0.038450960339436534	0.0
11	0.0	0.0	0.0	0.04299030982395335	0.0
12	0.0	0.0	0.0	0.049131782655946685	0.0
13	0.0	0.0	0.0	0.05100092656133596	0.0
14	0.0	0.0	0.0	0.05580729660376552	0.0
15	0.0	0.0	0.0	0.06728918059401394	0.0
16	0.0	0.0	0.0	0.08731572243747046	0.0
17	0.0	0.0	0.0	0.11001246986005453	0.0
18	0.0	0.0	0.0	0.12443157998734322	0.0
19	0.0	0.0	0.0	0.145259183504538	0.0
20	0.0	0.0	0.0	0.1596782936318267	0.0
21	0.0	0.0	0.0	0.19999839787665252	0.0
22	0.0	0.0	0.0	0.25927696173328385	0.0
23	0.0	0.0	0.0	0.3634149793192578	0.0
24	0.0	0.0	0.0	0.5142815945399636	2.670205579127537E-4
25	0.0	0.0	0.0	0.5554027604585277	2.670205579127537E-4
26	0.0	0.0	0.0	0.6202887560313268	2.670205579127537E-4
27	0.0	0.0	0.0	0.6603418397182399	2.670205579127537E-4
28	0.0	0.0	0.0	0.7052012934475825	2.670205579127537E-4
29	0.0	0.0	0.0	0.7797000291052408	2.670205579127537E-4
30	0.0	0.0	0.0	0.949792124495665	2.670205579127537E-4
31	0.0	0.0	0.0	1.4248216970224536	2.670205579127537E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGTGT	20	0.001840352	37.0	10
TTATGTG	25	0.005491671	29.6	34
CGTGTCT	25	0.005491671	29.6	12
CCCTGCG	35	8.858187E-4	26.428572	7
CGTATTA	40	0.0019287699	23.125002	15
CCTACAC	40	0.0019287699	23.125002	3
TCCGTAT	40	0.0019287699	23.125002	13
ACTCGCA	40	0.0019287699	23.125002	21
TACTCGC	40	0.0019287699	23.125002	20
CAGTCCT	40	0.0019287699	23.125002	14
TGACGCA	50	2.697391E-4	22.2	10
TTAATCT	60	3.717657E-5	21.583334	4
GACACAT	95	7.1067916E-9	21.421053	26
CACATGT	95	7.1067916E-9	21.421053	28
GCCGTCT	70	5.08678E-6	21.142859	36
ATCCGTA	45	0.003821066	20.555555	12
CCGTATT	45	0.003821066	20.555555	14
TCTGTTG	45	0.003821066	20.555555	8
ACATGTT	90	9.435462E-8	20.555555	29
AAGGCCA	45	0.003821066	20.555555	25
>>END_MODULE