FastQCFastQC Report
Fri 10 Feb 2017
ERR1630533.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630533.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83975
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT2200.261982732956237No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT1590.18934206609109855RNA PCR Primer, Index 46 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC1080.12860970526942542No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG1070.1274188746650789No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC1060.12622804406073235No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA990.11789222983030664No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC950.1131289074129205No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCTG250.005467727329.635
TCAGGTC400.001916394923.1253
TACACCA400.001916394923.1255
CTAAGGG603.67361E-521.58333228
CGGACAT705.0096223E-621.14285721
ATCTAAG705.0096223E-621.14285726
TCTAAGG705.0096223E-621.14285727
GACATCT705.0096223E-621.14285723
CTGGGGG450.003796782620.5555574
TAATCCC450.003796782620.5555575
GGGCATC656.805247E-519.92307732
GCATCAC656.805247E-519.92307734
AACGCAG1403.6379788E-1219.8214285
CATCTAA759.099189E-619.73333425
ATCACAG609.139967E-418.536
GTAATCC500.00698202118.54
ACAGGGC500.00698202118.58
GGCATCA701.202205E-418.533
AAGGGCA701.202205E-418.530
ACATCTA801.5877877E-518.524
Produced by FastQC (version 0.11.5)