##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630533.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	83975
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85251562965168	33.0	31.0	34.0	30.0	34.0
2	32.02060136945519	33.0	31.0	34.0	30.0	34.0
3	32.13654063709437	34.0	31.0	34.0	30.0	34.0
4	35.77424233402798	37.0	35.0	37.0	35.0	37.0
5	35.721381363501045	37.0	35.0	37.0	35.0	37.0
6	35.782661506400714	37.0	35.0	37.0	35.0	37.0
7	35.74247097350402	37.0	35.0	37.0	33.0	37.0
8	35.73116999106877	37.0	35.0	37.0	33.0	37.0
9	37.43916641857696	39.0	37.0	39.0	35.0	39.0
10	37.31958320928848	39.0	37.0	39.0	34.0	39.0
11	37.42280440607323	39.0	37.0	39.0	34.0	39.0
12	37.328847871390295	39.0	37.0	39.0	34.0	39.0
13	37.37423042572194	39.0	37.0	39.0	34.0	39.0
14	38.62128014289967	40.0	38.0	41.0	34.0	41.0
15	38.6495623697529	40.0	38.0	41.0	34.0	41.0
16	38.624685918428106	40.0	38.0	41.0	34.0	41.0
17	38.56752604941947	40.0	38.0	41.0	34.0	41.0
18	38.543054480500146	40.0	38.0	41.0	34.0	41.0
19	38.60938374516225	40.0	38.0	41.0	34.0	41.0
20	38.56958618636499	40.0	38.0	41.0	34.0	41.0
21	38.51853527835665	40.0	38.0	41.0	34.0	41.0
22	38.44130991366478	40.0	38.0	41.0	34.0	41.0
23	38.38225662399524	40.0	38.0	41.0	34.0	41.0
24	38.32497767192617	40.0	38.0	41.0	34.0	41.0
25	38.27109258707949	40.0	38.0	41.0	34.0	41.0
26	38.16702590056565	40.0	38.0	41.0	33.0	41.0
27	38.020732360821675	40.0	38.0	41.0	33.0	41.0
28	37.94691277165823	40.0	37.0	41.0	33.0	41.0
29	37.853635010419765	40.0	37.0	41.0	33.0	41.0
30	37.79461744566835	40.0	37.0	41.0	33.0	41.0
31	37.77077701696933	40.0	37.0	41.0	33.0	41.0
32	37.69096754986603	40.0	37.0	41.0	32.0	41.0
33	37.60147662994939	40.0	37.0	41.0	32.0	41.0
34	37.55626079190235	40.0	37.0	41.0	32.0	41.0
35	37.52858588865734	40.0	37.0	41.0	32.0	41.0
36	37.532896695445075	40.0	37.0	41.0	32.0	41.0
37	37.40392974099434	40.0	37.0	41.0	31.0	41.0
38	37.2716165525454	39.0	36.0	41.0	31.0	41.0
39	37.24094075617744	39.0	36.0	41.0	31.0	41.0
40	37.09459958320929	39.0	36.0	41.0	31.0	41.0
41	37.07615361714796	39.0	36.0	41.0	31.0	41.0
42	36.96758559094969	39.0	36.0	41.0	31.0	41.0
43	36.07177136052397	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	3.0
18	7.0
19	4.0
20	16.0
21	26.0
22	43.0
23	68.0
24	127.0
25	215.0
26	314.0
27	474.0
28	690.0
29	1002.0
30	1243.0
31	1677.0
32	2139.0
33	2783.0
34	3718.0
35	5036.0
36	7020.0
37	10714.0
38	20836.0
39	25819.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.93807680857398	17.53259898779399	11.459362905626675	27.069961298005357
2	20.482286394760347	19.661804108365587	32.335814230425726	27.520095266448347
3	21.34325692170289	20.63590354272105	28.419172372729978	29.601667162846084
4	15.8106579339089	14.339982137540936	32.160762131586786	37.68859779696338
5	15.698719857100327	35.09735040190533	34.1625483774933	15.041381363501042
6	38.73771955939267	32.64543018755582	13.888657338493598	14.728192914557905
7	31.705864840726406	28.22983030663888	19.377195593926764	20.68710925870795
8	29.15986900863352	32.03096159571301	18.767490324501342	20.04167907115213
9	27.449836260791905	12.337005061030068	17.10151830902054	43.11164036915749
10	17.405180113128907	25.657636201250373	32.407264066686515	24.52991961893421
11	38.91515331944031	19.25454004167907	19.078297112235784	22.752009526644834
12	23.658231616552545	23.390294730574578	27.159273593331353	25.792200059541532
13	33.97439714200655	17.34921107472462	22.281631437927953	26.394760345340874
14	24.852634712712117	18.562667460553737	22.36260791902352	34.222089907710625
15	28.221494492408457	25.23250967549866	19.973801726704377	26.57219410538851
16	28.358440011908304	23.75349806490027	20.654956832390592	27.233105090800834
17	25.72313188448943	24.629949389699316	22.668651384340578	26.978267341470673
18	26.650788925275382	22.197082465019353	23.898779398630545	27.253349211074724
19	26.917534980649005	22.896100029770768	23.46055373623102	26.725811253349214
20	28.03215242631736	22.260196487049715	23.302173265852932	26.405477820779993
21	28.332241738612684	22.93658827031855	22.00535873771956	26.725811253349214
22	28.19529621911283	23.003274784161952	22.48526347127121	26.316165525454004
23	27.462935397439715	22.679368859779697	22.5233700506103	27.334325692170292
24	27.284310806787737	23.147365287287883	22.7639178326883	26.80440607323608
25	27.627270020839532	23.081869604048823	22.218517415897587	27.07234295921405
26	27.292646621018164	23.30336409645728	22.33760047633224	27.06638880619232
27	27.383149746948497	22.91515331944031	22.925870794879426	26.775826138731766
28	27.092587079487945	22.5055075915451	22.807978565049122	27.593926763917832
29	25.814825841024113	23.27121167013992	23.562965168204823	27.350997320631144
30	26.565049121762428	23.84161952962191	23.31289074129205	26.28044060732361
31	27.21047930931825	23.839237868413218	21.94700803810658	27.003274784161952
32	26.40666865138434	23.537957725513543	22.896100029770768	27.159273593331353
33	26.590056564453707	22.844894313783865	23.591545102709137	26.97350401905329
34	26.675796367966658	22.23518904435844	23.730872283417686	27.35814230425722
35	26.16135754688895	22.729383745162252	24.292944328669247	26.81631437927955
36	25.938672223876154	23.59511759452218	23.47841619529622	26.987793986305448
37	26.760345340875258	22.387615361714797	23.895206906817506	26.95683239059244
38	25.733849359928552	22.432866924679963	24.70139922596011	27.13188448943138
39	25.88627567728491	22.582911580827627	24.36677582613873	27.164036915748735
40	24.768085739803514	21.69693361119381	26.481690979458172	27.053289669544505
41	24.389401607621316	21.587377195593927	26.362607919023517	27.660613277761236
42	24.79666567430783	21.2360821673117	27.02828222685323	26.93896993152724
43	23.759452217922	20.727597499255733	27.089014587674903	28.423935695147367
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	1.5
2	0.0
3	2.0
4	4.0
5	4.0
6	8.5
7	13.0
8	13.5
9	14.0
10	24.0
11	34.0
12	34.0
13	49.5
14	65.0
15	98.0
16	131.0
17	122.5
18	114.0
19	114.0
20	136.5
21	159.0
22	161.0
23	163.0
24	216.5
25	270.0
26	270.0
27	375.0
28	480.0
29	607.5
30	735.0
31	953.5
32	1172.0
33	1172.0
34	1347.5
35	1523.0
36	1872.0
37	2221.0
38	2565.0
39	2909.0
40	2909.0
41	3241.5
42	3574.0
43	3871.5
44	4169.0
45	4561.5
46	4954.0
47	4954.0
48	5231.5
49	5509.0
50	6096.5
51	6684.0
52	7079.5
53	7475.0
54	7475.0
55	7699.5
56	7924.0
57	7780.0
58	7636.0
59	7157.5
60	6679.0
61	6679.0
62	6248.0
63	5817.0
64	5171.0
65	4525.0
66	3929.5
67	3334.0
68	3334.0
69	2784.5
70	2235.0
71	1896.0
72	1557.0
73	1216.5
74	876.0
75	876.0
76	664.0
77	452.0
78	367.5
79	283.0
80	218.0
81	153.0
82	153.0
83	112.0
84	71.0
85	50.5
86	30.0
87	22.0
88	14.0
89	14.0
90	12.0
91	10.0
92	7.0
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	83975.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.74337600476333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.78216185436717	81.2694254242334
2	2.970709553768593	5.0943733253944625
3	0.8124661472438649	2.089907710628163
4	0.3874838548393817	1.3289669544507294
5	0.2541560768301321	1.0896100029770766
6	0.15416024332319486	0.79309318249479
7	0.10971765065344499	0.6585293242036321
8	0.0916628473813591	0.6287585590949688
9	0.06249739594183576	0.48228639476034535
>10	0.3583184033998584	5.170586484072641
>50	0.009721817146507784	0.5608812146472164
>100	0.006944155104648417	0.8335814230425722
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT	220	0.261982732956237	No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT	159	0.18934206609109855	RNA PCR Primer, Index 46 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC	108	0.12860970526942542	No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG	107	0.1274188746650789	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	106	0.12622804406073235	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	99	0.11789222983030664	No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC	95	0.1131289074129205	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0011908306043465317	0.0
3	0.0	0.0	0.0	0.003572491813039595	0.0
4	0.0	0.0	0.0	0.008335814230425722	0.0
5	0.0	0.0	0.0	0.010717475439118785	0.0
6	0.0	0.0	0.0	0.010717475439118785	0.0
7	0.0	0.0	0.0	0.010717475439118785	0.0
8	0.0	0.0	0.0	0.011908306043465317	0.0
9	0.0	0.0	0.0	0.015480797856504911	0.0
10	0.0	0.0	0.0	0.027389103899970228	0.0
11	0.0	0.0011908306043465317	0.0	0.030961595713009823	0.0
12	0.0	0.0011908306043465317	0.0	0.032152426317356354	0.0
13	0.0	0.0011908306043465317	0.0	0.03691574873474248	0.0
14	0.0	0.0011908306043465317	0.0	0.03691574873474248	0.0
15	0.0	0.0011908306043465317	0.0	0.03691574873474248	0.0
16	0.0	0.0011908306043465317	0.0	0.044060732360821676	0.0
17	0.0	0.0011908306043465317	0.0	0.051205715986900865	0.0
18	0.0	0.0011908306043465317	0.0	0.05596903840428699	0.0
19	0.0	0.0011908306043465317	0.0	0.05715986900863352	0.0
20	0.0	0.0011908306043465317	0.0	0.05954153021732658	0.0
21	0.0	0.0011908306043465317	0.0	0.06073236082167312	0.0
22	0.0	0.0011908306043465317	0.0	0.06311402203036617	0.0
23	0.0	0.0011908306043465317	0.0	0.06906817505209883	0.0
24	0.0	0.0011908306043465317	0.0	0.07621315867817803	0.0
25	0.0	0.0011908306043465317	0.0	0.07978565049121762	0.0
26	0.0	0.0011908306043465317	0.0	0.08216731169991069	0.0
27	0.0	0.0011908306043465317	0.0	0.09526644834772253	0.0
28	0.0	0.0011908306043465317	0.0	0.12622804406073235	0.0
29	0.0	0.0011908306043465317	0.0	0.17147960702590057	0.0
30	0.0	0.0011908306043465317	0.0	0.24769276570407858	0.0
31	0.0	0.0011908306043465317	0.0	0.47633224173861266	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTCTG	25	0.0054677273	29.6	35
TCAGGTC	40	0.0019163949	23.125	3
TACACCA	40	0.0019163949	23.125	5
CTAAGGG	60	3.67361E-5	21.583332	28
CGGACAT	70	5.0096223E-6	21.142857	21
ATCTAAG	70	5.0096223E-6	21.142857	26
TCTAAGG	70	5.0096223E-6	21.142857	27
GACATCT	70	5.0096223E-6	21.142857	23
CTGGGGG	45	0.0037967826	20.555557	4
TAATCCC	45	0.0037967826	20.555557	5
GGGCATC	65	6.805247E-5	19.923077	32
GCATCAC	65	6.805247E-5	19.923077	34
AACGCAG	140	3.6379788E-12	19.821428	5
CATCTAA	75	9.099189E-6	19.733334	25
ATCACAG	60	9.139967E-4	18.5	36
GTAATCC	50	0.006982021	18.5	4
ACAGGGC	50	0.006982021	18.5	8
GGCATCA	70	1.202205E-4	18.5	33
AAGGGCA	70	1.202205E-4	18.5	30
ACATCTA	80	1.5877877E-5	18.5	24
>>END_MODULE