FastQCFastQC Report
Fri 10 Feb 2017
ERR1630532.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630532.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948060
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG35310.3724447819758243No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT27710.29228107925658714No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG24870.26232516929308275No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA24190.25515262747083517No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC23270.2454486002995591No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA20610.21739130434782608No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG20350.2146488618863785No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT19790.20874206273864523No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC19380.2044174419340548No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA19360.20420648482163575No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG18970.20009282112946442No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC18970.20009282112946442No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG18320.1932367149758454No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT17660.18627513026601691No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG16860.17783684576925513No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA15960.16834377571039807No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA15830.1669725544796743No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA15660.1651794190241124No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC15440.1628588907875029No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG15080.15906166276396008No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC15030.15853426998291248No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG14930.15747948442081725No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC14430.1522055566103411No Hit
CGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAG14330.1511507710482459No Hit
GAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGGCGCCGA14260.15041242115477924No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA13480.14218509377043648No Hit
AGTCTGGGGTCCGAGGGAGCGCCGGTGACACAGCCCAGGGCCA12670.13364133071746515No Hit
TAGCAGATCTCTGCAGCTCAAGCCTCATTTCATCCTGCTCAGC12670.13364133071746515No Hit
GTTCGGAGCCATCGCTGCTGCCTGCTGATCCGCGCCTAGAGTT12590.13279750226778897No Hit
GGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAGTGGCT12380.130582452587389No Hit
CTCCAGCTCGGCTTTCGCGGCGCCGAGATGCTGTCCTGCCGCC12230.12900027424424615No Hit
GCCAGGGACTTCTGCAGAAACTGACGGAGTCTGGGGTCCGAGG12060.12720713878868428No Hit
GTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAGCCAGGGAC11520.12151129675337005No Hit
CCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGGCTGCTGCC11500.12130033964095098No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC11230.11845241862329388No Hit
CGCCTAGAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGG11070.11676476172394151No Hit
GTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTC10920.11518258338079869No Hit
GCCATAGCCGGGTTTGAGTTAGCAGATCTCTGCAGCTCAAGCC10750.1133894479252368No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG10500.11075248401999874No Hit
CTGCAGCTCAAGCCTCATTTCATCCTGCTCAGCAGCCTGGGAC10190.10748264877750353No Hit
GTCCGAGGGAGCGCCGGTGACACAGCCCAGGGCCAGGACGATG10080.10632238465919877No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG10070.10621690610298927No Hit
GTCAAACTCTAGGCGCGGATCAGCAGGCAGCAGCGATGGCTCC10010.10558403476573212No Hit
GCTTTGCGTTCTCGGGGTGCCATAGCCGGGTTTGAGTTAGCAG9880.10421281353500833No Hit
CTCTAGGCGCGGATCAGCAGGCAGCAGCGATGGCTCCGAACCT9860.1040018564225893No Hit
CTGGAGGCGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGAGC9860.1040018564225893No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG9850.10389637786637977No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA9600.1012594139611417No Hit
GATCTTCAGGTTCCAGGGCATCATTCTCCGTCTGGTTGGGTTC9560.10083749973630361No Hit
GTCCCTGGCTGCTGCCGCGGGGAAGCAGGAACTGGCCAAGTAC9540.10062654262388455No Hit
GCTCAGCAGCCTGGGACAGATCTTCAGGTTCCAGGGCATCATT9520.10041558551146551No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCGG358.8682433E-426.42857216
TACAGTA451.3229733E-424.6666662
GGTATCA6250.020.4241
TATAACA555.142181E-420.1818182
CTAGACA656.9005015E-519.9230774
CAATATA500.007033995418.536
CCTACAC1054.7950743E-717.619053
TCTAGCG1800.017.47222128
TAATGCT752.0667855E-417.2666664
ATTGGCG650.001579658117.0769237
TAATACT1107.8040466E-716.8181824
TACACAT2100.016.7380965
CTAGCGG2000.016.65000229
ACGGACC1606.2937033E-1016.18758
GCCGTCT1151.2415567E-616.08695636
GTACAAG700.002592044215.8571431
ACTAGTC700.002592044215.8571433
GTATAGA700.002592044215.8571431
CTTATAC4450.015.79775237
CGCGGTC957.0575916E-515.57894810
Produced by FastQC (version 0.11.5)