FastQCFastQC Report
Fri 10 Feb 2017
ERR1630531.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630531.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487027
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT87791.8025694674011914No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT71661.4713763302650573No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT65921.3535183880975798No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50061.0278690914466755No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14250.2925915811649046No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13820.28376250187361274No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13730.28191455504520285No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9080.1864373022440234No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT8830.18130411660955142No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6900.1416759235114275No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6480.13305217164551453No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG6410.13161487966786234No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6050.12422309235422266No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT5690.11683130504058298No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA5510.11313541138376311No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5110.10492231436860791No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA5060.1038956772417135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTAGG509.077046E-625.9000021
CCTAATC400.00192959823.12536
AAATACG555.137247E-420.1818185
GTCGGGA851.2422952E-619.5882342
GCAGTCG2100.019.380959
CAGTCGG2050.018.95121810
CTTCGTC500.007029202818.521
ATAATAT500.007029202818.53
CTGACTA609.226396E-418.535
ACCTATG609.226396E-418.57
CAAGGCA500.007029202818.54
ACTCCGT701.2173675E-418.58
GCCGTCT902.1470369E-618.536
GACTCCG701.2173675E-418.57
ATACACT500.007029202818.54
CCATTCC701.2173675E-418.59
CAGACAC500.007029202818.531
ATACTGG1552.0008883E-1117.9032256
GGCAGTC2500.017.768
GGGCAGT2500.017.767
Produced by FastQC (version 0.11.5)