FastQCFastQC Report
Fri 10 Feb 2017
ERR1630530.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630530.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107962
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20541.9025212574794836No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT18231.6885570849002427No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17531.6237194568459272No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT15021.3912302476797391RNA PCR Primer, Index 26 (100% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11191.0364757970396992No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG8480.7854615512865638No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC8260.7650840110409218No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC8180.7576739964061429No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8020.742853967136585No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC3120.28899057075637724RNA PCR Primer, Index 26 (100% over 23bp)
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA2990.27694929697486154No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG2300.21303792074989347No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2080.1926603805042515No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2020.18710286952816732No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.1806191067227358No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1700.1574628109890517No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT1550.14356903354884126No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1520.14079027806079916No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1430.1324540115966729No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA1320.12226524147385193No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA1310.12133898964450454No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1300.12041273781515718No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1200.11115021952168357No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA1150.10651896037494674No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1130.10466645671625202No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA1130.10466645671625202No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCAAG250.00547458329.59999828
TTGAACC250.00547458329.5999984
CGTCTTC556.17385E-726.9090937
TAGGACC358.817223E-426.4285724
TATACAC3000.025.2833333
CCGTCTT2150.024.95349137
TTATACA3100.024.4677432
GCCGTCT2350.022.82978836
TACACAT3400.022.3088255
ATACACA3500.021.6714294
CTTATAC3550.021.3661981
GCTTATT705.031634E-621.14285927
CTCCGCT806.84071E-720.812523
AGTCGGT2050.018.95121811
GCAGTCG2100.018.59
CCGCTTA902.1157084E-618.525
CAGTTCC500.006994675418.4999984
TGCCGTC2950.018.18644135
GGGCAGT2150.018.0697697
ATGCCGT3000.017.88333334
Produced by FastQC (version 0.11.5)