FastQCFastQC Report
Fri 10 Feb 2017
ERR1630529.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630529.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416782
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT70211.684573709997073No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT62021.4880681027491591No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42891.029075151997927No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35700.8565629033883421No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC19370.464751356824431No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT15310.36733832075281564No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT12200.2927189753876127TruSeq Adapter, Index 12 (95% over 22bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10620.254809468739053No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT10300.24713159397478776No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT9990.23969365279690583No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG9700.2327355787917904No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG9140.21929929795432623No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9070.21761976284964324No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8860.21258115753559412No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG8310.19938481028451324No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC7800.1871481973789655No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT6960.16699377612276922No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT6830.16387463949978648No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT6790.1629149051542533No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG6780.16267497156787No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG6750.16195517080872013No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT6640.15931590135850396No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT6590.15811623342658754No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6490.1557168975627546No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG6400.15355749528530502No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG6400.15355749528530502No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA6370.15283769452615517No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC6160.14779908921210608No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG6140.1473192220393395No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC6110.14659942128018966No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG6040.14491988617550663No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC6040.14491988617550663No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5830.13988128086145754No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA5750.13796181217039125No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT5560.13340307402910875No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA5380.12908426947420953No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG5080.12188626188271087No Hit
TTGCAGTAGTTCTCCAGCTGGTAGAGGGAGCAGATGCTGGTAC5030.12068659395079442No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT4990.11972685960526126No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA4860.1166077229822785No Hit
GCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACG4850.1163677893958952No Hit
TTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCT4850.1163677893958952No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA4830.11588792222312863No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4770.11444832070482891No Hit
ACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCAAGAC4560.10940971539077982No Hit
GAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGA4550.10916978180439654No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA4430.10629057876779706No Hit
CACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCT4360.10461104366311406No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA4320.10365130931758089No Hit
CCTGCGGGCTGCGTCTAGTTGCAGTAGTTCTCCAGCTGGTAGA4200.10077210628098143No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAGT200.001840589937.01
TAAGTAA405.9311842E-527.75000226
ATACTGG556.245664E-726.909096
TCGCACG358.859872E-426.42857222
TGCTAGA358.859872E-426.42857216
ATCGCAC358.859872E-426.42857221
GCCGTCT1700.026.11764736
CTATAGA451.321236E-424.6666663
CCGTCTT1800.024.66666637
ACTAATG451.321236E-424.66666613
AGTAACA451.321236E-424.66666628
CAGACTG451.321236E-424.66666633
AATACTG551.8988881E-523.5454545
CTAGATA400.00192913323.1250023
CAAGCCT400.00192913323.12500232
AAGTAAC502.6981003E-422.19999927
ATAAGTA502.6981003E-422.19999925
CCTATAG502.6981003E-422.1999992
CTAATGT502.6981003E-422.19999914
TAACATG603.7189548E-521.58333230
Produced by FastQC (version 0.11.5)