FastQCFastQC Report
Fri 10 Feb 2017
ERR1630528.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630528.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131897
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28772.181247488570627No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT25471.9310522604759774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25351.9219542521816266No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT20981.5906351167956814RNA PCR Primer, Index 17 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17711.3427143907746197No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC12310.9333040175288293No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG12000.9098008294350896No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11260.8536964449532589No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC10870.8241279179966186No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC5080.3851490177941879RNA PCR Primer, Index 17 (95% over 22bp)
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA3900.2956852695664041No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG3590.27218208147266426No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3050.23124104414808522No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2930.22214303585373435No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2890.21911036642228407No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2580.19560717832854424No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT2230.16907132080335413No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA2060.15618247571969035No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.15466614100396522No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2010.1523916389303775No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA2010.1523916389303775No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA1780.13495378969953828No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA1720.13040478555236282No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1720.13040478555236282No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC1610.1220649446158745No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.12054860990014936No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA1490.11296693632152362No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA1470.11145060160579848No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG1400.10614343010076045No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA1350.10235259331144757No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAT250.005478930729.65
CGTCTTC803.092282E-1127.75000237
TTATACA3950.025.7594932
CCGTCTT2750.025.56363537
TATACAC4000.024.9753
GCCGTCT2950.023.83051136
GAACAGC400.001922182323.1250026
CTTATAC4400.023.1251
CGTCTGT450.0038081420.55555536
ATGCCCC450.0038081420.55555537
ATACACA5000.019.984
TACACAT5050.019.7821795
ATGGGGC500.00700270518.512
CAAAAAT500.00700270518.524
AATGGTA1205.0513336E-918.510
GGGAATG1409.094947E-1118.57
TGCCGTC3900.018.535
ATGCCGT3900.018.02564234
GAATGGT1258.378265E-917.769
TATGCCG3950.017.32911333
Produced by FastQC (version 0.11.5)