##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630528.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	131897
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.853044421025498	33.0	31.0	34.0	30.0	34.0
2	31.960635950779775	33.0	31.0	34.0	30.0	34.0
3	31.971985716126976	33.0	31.0	34.0	30.0	34.0
4	35.66051540216988	37.0	35.0	37.0	33.0	37.0
5	35.69012941916799	37.0	35.0	37.0	35.0	37.0
6	35.756196122732135	37.0	35.0	37.0	35.0	37.0
7	35.727757265138706	37.0	35.0	37.0	35.0	37.0
8	35.77031319893553	37.0	35.0	37.0	35.0	37.0
9	37.38523241620355	39.0	37.0	39.0	34.0	39.0
10	37.33586813953312	39.0	37.0	39.0	34.0	39.0
11	37.47522688158184	39.0	37.0	39.0	35.0	39.0
12	37.39887942864508	39.0	37.0	39.0	34.0	39.0
13	37.47887366657316	39.0	37.0	39.0	35.0	39.0
14	38.537661963501826	40.0	38.0	41.0	34.0	41.0
15	38.51123224940673	40.0	38.0	41.0	34.0	41.0
16	38.373071411783435	40.0	38.0	41.0	34.0	41.0
17	38.45101101617171	40.0	38.0	41.0	34.0	41.0
18	38.57378863810398	40.0	38.0	41.0	34.0	41.0
19	38.61805803012957	40.0	38.0	41.0	34.0	41.0
20	38.64683048136045	40.0	38.0	41.0	34.0	41.0
21	38.5641674943327	40.0	38.0	41.0	34.0	41.0
22	38.42733344958566	40.0	38.0	41.0	34.0	41.0
23	38.396468456447074	40.0	38.0	41.0	34.0	41.0
24	38.30183400683867	40.0	38.0	41.0	34.0	41.0
25	38.210186736620244	40.0	37.0	41.0	33.0	41.0
26	37.89030834666444	40.0	37.0	41.0	33.0	41.0
27	37.66423800389698	40.0	36.0	41.0	33.0	41.0
28	37.53005754490246	40.0	36.0	41.0	32.0	41.0
29	37.334033374527095	39.0	35.0	41.0	32.0	41.0
30	37.125370554296154	39.0	35.0	41.0	32.0	41.0
31	36.872483832081095	39.0	35.0	41.0	31.0	41.0
32	36.579406658225736	38.0	35.0	41.0	31.0	41.0
33	36.38578587837479	38.0	35.0	40.0	31.0	41.0
34	36.19297633759676	38.0	35.0	40.0	30.0	41.0
35	35.97422989150625	38.0	35.0	40.0	30.0	41.0
36	35.73512665185713	38.0	35.0	40.0	29.0	41.0
37	35.35006103247231	38.0	35.0	40.0	27.0	41.0
38	35.0178775862984	37.0	34.0	40.0	26.0	41.0
39	34.69551240740881	37.0	34.0	40.0	24.0	41.0
40	34.28609445248944	37.0	33.0	40.0	22.0	41.0
41	33.93616230846797	37.0	33.0	40.0	20.0	41.0
42	33.587291598747505	36.0	33.0	40.0	18.0	41.0
43	32.540368620969396	35.0	32.0	39.0	14.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	0.0
14	2.0
15	1.0
16	2.0
17	2.0
18	4.0
19	16.0
20	31.0
21	62.0
22	124.0
23	243.0
24	364.0
25	624.0
26	905.0
27	1311.0
28	1887.0
29	2399.0
30	2924.0
31	3656.0
32	4619.0
33	5689.0
34	7221.0
35	9267.0
36	12910.0
37	21317.0
38	27060.0
39	29254.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.0336247223212	18.506107038067583	14.568943948687233	21.89132429092398
2	18.502316201278273	21.728318308983525	33.5109972175258	26.258368272212408
3	22.367453391661677	24.91110487729061	31.667134203204018	21.054307527843697
4	13.348294502528487	18.250604638467895	27.6806902355626	40.72041062344102
5	12.92523711684117	39.396650416612964	30.84603895463885	16.83207351190702
6	31.645905517183863	28.713314176971426	18.25818631204652	21.382593993798192
7	26.182551536426153	33.91130958247723	18.241506630173546	21.66463225092307
8	34.13875978983601	28.16743367931037	18.713844894121927	18.979961636731694
9	27.544220111147332	10.979779676565805	19.14145128395642	42.33454892833044
10	21.698749782026887	28.003669530012054	23.742768978824387	26.55481170913668
11	31.08334533764983	22.743504401161513	17.88971697612531	28.283433285063346
12	21.187744982827507	30.67317679704618	23.918663805848503	24.22041441427781
13	34.07355739705983	16.551551589497866	26.92176471034216	22.453126303100145
14	24.785249095885426	22.405361759554804	25.262894531338848	27.546494613220922
15	31.92415293751943	22.111192824704126	22.44933546631083	23.515318771465612
16	26.589687407598355	22.45767530724732	26.902810526395598	24.049826758758726
17	19.622887556199156	31.99011349765347	25.379652304449685	23.007346641697687
18	22.462224311394497	20.462178821353024	29.043116977641642	28.032479889610833
19	21.948186842763672	28.201551210414188	29.829336527745138	20.020925419077006
20	24.25983911688666	20.63125014215638	31.961303138054692	23.147607602902266
21	28.308452807872808	21.230960522225676	28.609445248944255	21.851141420957262
22	24.798137940969088	24.873954676755346	27.565448797167484	22.762458585108078
23	23.992964206919034	24.410714421101314	27.827016535630072	23.769304836349576
24	23.90274229133339	25.714762276624942	29.31529905911431	21.06719637292736
25	21.3257314419585	28.02944722017938	28.526046839579365	22.11877449828275
26	25.343260271272282	25.598762670871967	28.368348029143952	20.6896290287118
27	20.78439994844462	28.218230892287167	28.482073132823338	22.51529602644488
28	21.022464498813466	25.709455105119904	30.838457281060222	22.429623115006407
29	20.32267602750631	27.585919315829777	30.181884349151233	21.909520307512683
30	20.68128918777531	27.667801390478935	32.66336611143544	18.98754331031032
31	22.227192430457098	26.978627262181853	28.265995435832508	22.52818487152854
32	19.266548898003748	28.11966913576503	31.097750517449224	21.516031448782005
33	18.50838154014117	28.780032904463333	32.208465696717894	20.503119858677604
34	21.501626268982616	28.99686876881203	28.158335671016022	21.343169291189337
35	18.5462899080343	29.12348271757508	32.14023063451027	20.18999673988036
36	19.296875592318248	29.590513810018425	28.01125120359068	23.10135939407265
37	19.15585646375581	28.88617633456409	29.626147675837966	22.331819525842135
38	18.841975177600702	26.12568898458646	31.402533795309978	23.629802042502863
39	21.130124263629956	26.22045990431928	29.433573166940867	23.215842665109896
40	18.279414998066674	25.628331197828608	33.658081684951135	22.43417211915358
41	18.689583538670327	27.39561930900627	29.182619771488362	24.732177380835047
42	18.650158836061472	25.873219254418224	32.63910475598383	22.83751715353647
43	17.011001008362587	27.13253523582796	32.28352426514629	23.57293949066317
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	20.0
2	37.0
3	132.5
4	228.0
5	228.0
6	404.0
7	580.0
8	612.0
9	644.0
10	874.0
11	1104.0
12	1104.0
13	1987.0
14	2870.0
15	4268.5
16	5667.0
17	5068.5
18	4470.0
19	4470.0
20	5078.0
21	5686.0
22	4018.0
23	2350.0
24	2097.5
25	1845.0
26	1845.0
27	2045.5
28	2246.0
29	2271.5
30	2297.0
31	2230.5
32	2164.0
33	2164.0
34	2159.5
35	2155.0
36	2161.0
37	2167.0
38	2502.5
39	2838.0
40	2838.0
41	3255.0
42	3672.0
43	4177.5
44	4683.0
45	5699.5
46	6716.0
47	6716.0
48	8460.0
49	10204.0
50	11371.5
51	12539.0
52	9423.5
53	6308.0
54	6308.0
55	7662.5
56	9017.0
57	8447.0
58	7877.0
59	6824.0
60	5771.0
61	5771.0
62	5669.0
63	5567.0
64	5304.5
65	5042.0
66	4835.5
67	4629.0
68	4629.0
69	4240.0
70	3851.0
71	3252.0
72	2653.0
73	2225.5
74	1798.0
75	1798.0
76	1473.0
77	1148.0
78	867.5
79	587.0
80	441.0
81	295.0
82	295.0
83	212.0
84	129.0
85	84.5
86	40.0
87	25.5
88	11.0
89	11.0
90	7.5
91	4.0
92	3.0
93	2.0
94	2.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	131897.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.33939361774718
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.74731131235357	46.345254251423455
2	9.545012398788892	10.564303964457114
3	2.7839048649833544	4.621788213530255
4	1.1302763354386158	2.5019522809464965
5	0.6589853543587565	1.823392495659492
6	0.43978024687975226	1.4602303312433185
7	0.2863366716444493	1.1091988445529466
8	0.19728459673110385	0.873408796257686
9	0.14522338370484034	0.7232916594008961
>10	0.9343617706292556	9.794005928868739
>50	0.07261169185242017	2.8658726127205316
>100	0.04521105341754463	4.44361888443255
>500	0.0013700319217437766	0.3851490177941879
>1k	0.01233028729569399	12.488532718712328
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2877	2.181247488570627	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT	2547	1.9310522604759774	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2535	1.9219542521816266	No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	2098	1.5906351167956814	RNA PCR Primer, Index 17 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1771	1.3427143907746197	No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC	1231	0.9333040175288293	No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG	1200	0.9098008294350896	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1126	0.8536964449532589	No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC	1087	0.8241279179966186	No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC	508	0.3851490177941879	RNA PCR Primer, Index 17 (95% over 22bp)
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	390	0.2956852695664041	No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG	359	0.27218208147266426	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	305	0.23124104414808522	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	293	0.22214303585373435	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	289	0.21911036642228407	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	258	0.19560717832854424	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	223	0.16907132080335413	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	206	0.15618247571969035	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	204	0.15466614100396522	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	201	0.1523916389303775	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	201	0.1523916389303775	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	178	0.13495378969953828	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	172	0.13040478555236282	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	172	0.13040478555236282	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	161	0.1220649446158745	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	159	0.12054860990014936	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	149	0.11296693632152362	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	147	0.11145060160579848	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	140	0.10614343010076045	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	135	0.10235259331144757	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0030326694314502982	0.0
3	0.0	0.0	0.0	0.0060653388629005964	0.0
4	0.0	0.0	0.0	0.007581673578625746	0.0
5	0.0	0.0	0.0	0.007581673578625746	0.0
6	0.0	0.0	0.0	0.00833984093648832	0.0
7	0.0	0.0	0.0	0.009856175652213469	0.0
8	0.0	0.0	0.0	0.01137251036793862	0.0
9	0.0	0.0	0.0	0.013647012441526343	7.581673578625746E-4
10	0.0	0.0	0.0	0.01819601658870179	7.581673578625746E-4
11	0.0	0.0	0.0	0.020470518662289515	7.581673578625746E-4
12	0.0	0.0	0.0	0.02274502073587724	7.581673578625746E-4
13	0.0	0.0	0.0	0.025777690167327536	7.581673578625746E-4
14	0.0	0.0	0.0	0.025777690167327536	7.581673578625746E-4
15	0.0	0.0	0.0	0.02956852695664041	7.581673578625746E-4
16	0.0	0.0	0.0	0.04018286996671645	0.0015163347157251491
17	0.0	0.0	0.0	0.05762071919755567	0.002274502073587724
18	0.0	0.0	0.0	0.06444422541831885	0.002274502073587724
19	0.0	0.0	0.0	0.08112390729129548	0.002274502073587724
20	0.0	0.0	0.0	0.09098008294350896	0.002274502073587724
21	0.0	0.0	0.0	0.10841793217434817	0.002274502073587724
22	0.0	0.0	0.0	0.14935896949892719	0.002274502073587724
23	0.0	0.0	0.0	0.22138486849587177	0.002274502073587724
24	0.0	0.0	0.0	0.31691395558655616	0.002274502073587724
25	0.0	0.0	0.0	0.33890080896457087	0.002274502073587724
26	0.0	0.0	0.0	0.3737765074262493	0.002274502073587724
27	0.0	0.0	0.0	0.4238155530451792	0.002274502073587724
28	0.0	0.0	0.0	0.4806781048848723	0.002274502073587724
29	0.0	0.0	0.0	0.5822725308384573	0.002274502073587724
30	0.0	0.0	0.0	0.7263243288323464	0.0030326694314502982
31	0.0	0.0	0.0	1.190322751844242	0.0030326694314502982
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGAT	25	0.0054789307	29.6	5
CGTCTTC	80	3.092282E-11	27.750002	37
TTATACA	395	0.0	25.759493	2
CCGTCTT	275	0.0	25.563635	37
TATACAC	400	0.0	24.975	3
GCCGTCT	295	0.0	23.830511	36
GAACAGC	40	0.0019221823	23.125002	6
CTTATAC	440	0.0	23.125	1
CGTCTGT	45	0.00380814	20.555555	36
ATGCCCC	45	0.00380814	20.555555	37
ATACACA	500	0.0	19.98	4
TACACAT	505	0.0	19.782179	5
ATGGGGC	50	0.007002705	18.5	12
CAAAAAT	50	0.007002705	18.5	24
AATGGTA	120	5.0513336E-9	18.5	10
GGGAATG	140	9.094947E-11	18.5	7
TGCCGTC	390	0.0	18.5	35
ATGCCGT	390	0.0	18.025642	34
GAATGGT	125	8.378265E-9	17.76	9
TATGCCG	395	0.0	17.329113	33
>>END_MODULE