FastQCFastQC Report
Fri 10 Feb 2017
ERR1630527.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630527.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1118607
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT66990.5988698443689339No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT61120.5463938630814933No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48890.43706145232418536No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35170.3144089032162323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA1002.874749E-718.53
TAATACT1800.018.54
AAGACGG2650.018.1509445
CTAGCGG1851.8189894E-1217.00000229
GCGCAAG2850.016.8771931
CGCAAGA2850.016.8771932
ACGGACC2650.016.7547178
CGGACCA2650.016.7547179
CAAGACG3000.016.654
TCTAGCG1801.0913936E-1116.44444528
GACGGAC2750.016.1454547
TTAATCG1151.242046E-616.08695637
CCGGTCG1659.731593E-1015.69697120
TGGACTA957.059403E-515.5789485
TCGCCAT2150.015.48837213
AACGCCG1802.0190782E-1015.4166665
CGTCGTA1802.0190782E-1015.41666610
TAACGCC1701.4861143E-915.2352944
AATAACG1701.4861143E-915.2352942
TGCGTAC855.363991E-415.23529411
Produced by FastQC (version 0.11.5)