##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630527.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1118607
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.451071734755818	31.0	31.0	34.0	30.0	34.0
2	31.615452075661963	31.0	31.0	34.0	30.0	34.0
3	31.69437166046699	31.0	31.0	34.0	30.0	34.0
4	35.41025936723085	37.0	35.0	37.0	33.0	37.0
5	35.29714278562533	37.0	35.0	37.0	33.0	37.0
6	35.33437212533088	37.0	35.0	37.0	32.0	37.0
7	35.30832812596381	37.0	35.0	37.0	32.0	37.0
8	35.307742576257795	37.0	35.0	37.0	32.0	37.0
9	36.92907160423634	39.0	37.0	39.0	33.0	39.0
10	36.74437134757783	38.0	37.0	39.0	32.0	39.0
11	36.90655610057867	39.0	37.0	39.0	33.0	39.0
12	36.822200290182344	38.0	37.0	39.0	33.0	39.0
13	36.89927382896764	39.0	37.0	39.0	33.0	39.0
14	38.00445464761082	40.0	37.0	41.0	33.0	41.0
15	37.943974961715774	40.0	37.0	41.0	33.0	41.0
16	37.90799628466477	40.0	37.0	41.0	33.0	41.0
17	37.90034301591175	40.0	37.0	41.0	33.0	41.0
18	37.87470309054029	40.0	37.0	41.0	33.0	41.0
19	37.90975114584479	40.0	37.0	41.0	33.0	41.0
20	37.888106368009495	40.0	37.0	41.0	33.0	41.0
21	37.81019875613151	40.0	37.0	41.0	33.0	41.0
22	37.74107081396773	39.0	37.0	41.0	33.0	41.0
23	37.69311205812229	39.0	37.0	41.0	32.0	41.0
24	37.62927373063104	39.0	37.0	41.0	32.0	41.0
25	37.56876901360353	39.0	37.0	41.0	32.0	41.0
26	37.30978261355418	39.0	36.0	40.0	32.0	41.0
27	37.160124154417055	39.0	36.0	40.0	31.0	41.0
28	37.01787491049135	39.0	36.0	40.0	31.0	41.0
29	36.88603861767359	39.0	36.0	40.0	31.0	41.0
30	36.77463309276627	39.0	35.0	40.0	30.0	41.0
31	36.69270977206472	39.0	35.0	40.0	30.0	41.0
32	36.57078044389137	39.0	35.0	40.0	30.0	41.0
33	36.49757510904187	38.0	35.0	40.0	30.0	41.0
34	36.40195797094064	38.0	35.0	40.0	30.0	41.0
35	36.31752974905396	38.0	35.0	40.0	30.0	41.0
36	36.243617284712144	38.0	35.0	40.0	30.0	41.0
37	35.99304581501814	38.0	35.0	40.0	29.0	41.0
38	35.83336238732638	38.0	35.0	40.0	28.0	41.0
39	35.72906123419575	38.0	35.0	40.0	28.0	41.0
40	35.57688357036922	38.0	34.0	40.0	27.0	41.0
41	35.3903596169164	38.0	34.0	40.0	27.0	41.0
42	35.255659941337754	38.0	34.0	40.0	26.0	41.0
43	34.341788492294434	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	2.0
10	10.0
11	4.0
12	7.0
13	0.0
14	4.0
15	9.0
16	25.0
17	46.0
18	104.0
19	181.0
20	408.0
21	679.0
22	1262.0
23	2109.0
24	3165.0
25	4956.0
26	7277.0
27	10374.0
28	14382.0
29	19533.0
30	25181.0
31	31874.0
32	39979.0
33	50770.0
34	65704.0
35	86660.0
36	123088.0
37	187523.0
38	283198.0
39	160090.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.2442314414267	19.98861083472569	12.674245735991283	24.092911987856326
2	19.194945141591283	22.07728004562818	33.8302013128829	24.89757349989764
3	21.338057065618223	24.16487649371048	29.230373133727934	25.266693306943367
4	15.17476647294358	16.16644630330402	33.291584980247755	35.36720224350465
5	14.307974114233149	37.73613074118077	33.386345696030865	14.569549448555211
6	34.72113083504752	34.1096560275414	15.733944092965627	15.435269044445457
7	28.42052660138905	31.050672845780515	21.61724358957167	18.911556963258764
8	27.60013123465167	32.97887461816348	20.084176122623944	19.336818024560905
9	26.667810947008196	13.561867572793663	20.54591111981241	39.22441036038573
10	17.436150497896044	27.2286871081622	32.24608821507464	23.089074178867108
11	34.83779379174277	22.24212793233012	21.234535453470254	21.68554282245686
12	21.33600093687953	26.398726272944835	28.92007648798908	23.345196302186558
13	29.61308126982935	19.974933108768315	26.28233150695463	24.129654114447703
14	22.918326096654145	21.641470149927542	25.929481936015065	29.51072181740325
15	25.86475857919716	27.4931231433381	23.385156717238495	23.256961560226248
16	24.835442653228522	26.410526663966884	25.245863828851416	23.508166853953174
17	23.057070088064886	27.017263435683848	26.297528980240603	23.628137496010662
18	22.660594829104415	25.080479560739384	28.241017622811228	24.017907987344973
19	23.401695144049697	25.82479816414523	28.22975361319927	22.543753078605803
20	23.974371696225752	24.712521913415525	28.217059253160404	23.09604713719832
21	24.47874901551662	25.369410346976196	27.240219308479208	22.91162132902798
22	24.2410426539437	25.256770250856647	27.34025444146157	23.161932653738088
23	23.175073998285367	25.53685074382692	27.73261744294466	23.55545781494305
24	23.42636868891398	26.238169437523634	27.41248713802077	22.922974735541615
25	23.567347602866782	25.56635172138204	27.45593403223831	23.41036664351287
26	23.392666056979795	26.398547479141467	27.398630618260032	22.810155845618702
27	23.855563213890132	25.65610621067095	27.021196899357864	23.467133676081055
28	22.836974916123356	26.010028544430707	28.06687245833434	23.0861240811116
29	22.645844340326853	26.248092493610358	28.089489874459932	23.01657329160286
30	22.432990317421577	27.016458863568705	27.968446469582258	22.582104349427457
31	23.129928562935866	26.62650957843103	27.218406464468753	23.025155394164347
32	22.44416493013185	26.463628423566092	27.386919624139665	23.705287022162384
33	22.21423609900528	26.338204570505997	27.932598312007702	23.51496101848102
34	23.167743452347427	26.167098900686298	27.603707110718958	23.061450536247317
35	23.084067952372905	26.235755721178215	27.99937779756429	22.680798528884587
36	22.299788933915128	26.65940763825007	27.537374609670778	23.50342881816402
37	23.108473306532144	25.971140892198957	27.55587976831899	23.36450603294991
38	22.397678541257118	25.744162158827898	28.331397890411914	23.52676140950307
39	22.72031195942811	25.761683951557607	28.00599316828877	23.51201092072551
40	22.170074029574284	25.7456819061565	28.707848243395578	23.37639582087364
41	21.563337257857317	26.006542065265105	29.015373585182285	23.414747091695297
42	22.396516381535246	25.79753210913216	28.694081120536524	23.111870388796067
43	21.70109788334956	25.38648515519749	28.762916734831805	24.149500226621146
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	219.0
1	193.0
2	167.0
3	529.0
4	891.0
5	891.0
6	1408.5
7	1926.0
8	2022.0
9	2118.0
10	2989.0
11	3860.0
12	3860.0
13	6774.0
14	9688.0
15	13314.5
16	16941.0
17	15819.5
18	14698.0
19	14698.0
20	16526.0
21	18354.0
22	16262.5
23	14171.0
24	15376.0
25	16581.0
26	16581.0
27	18474.5
28	20368.0
29	23951.0
30	27534.0
31	30411.5
32	33289.0
33	33289.0
34	36824.5
35	40360.0
36	43926.0
37	47492.0
38	53061.0
39	58630.0
40	58630.0
41	61745.0
42	64860.0
43	64756.5
44	64653.0
45	67366.0
46	70079.0
47	70079.0
48	72409.5
49	74740.0
50	75361.5
51	75983.0
52	78599.5
53	81216.0
54	81216.0
55	75912.5
56	70609.0
57	67706.0
58	64803.0
59	60028.0
60	55253.0
61	55253.0
62	51227.0
63	47201.0
64	42230.0
65	37259.0
66	32525.0
67	27791.0
68	27791.0
69	23980.0
70	20169.0
71	17473.5
72	14778.0
73	11738.0
74	8698.0
75	8698.0
76	6836.5
77	4975.0
78	4089.0
79	3203.0
80	2590.5
81	1978.0
82	1978.0
83	1558.0
84	1138.0
85	972.5
86	807.0
87	748.0
88	689.0
89	689.0
90	526.5
91	364.0
92	212.0
93	60.0
94	35.5
95	11.0
96	11.0
97	7.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1118607.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.4384301525356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.70967963562175	52.13325666723178
2	7.4621941846641215	8.870822156596315
3	1.8630123906710372	3.3220359556862644
4	0.7783412147647207	1.8505351971453026
5	0.4469997835822674	1.3284482707326566
6	0.28559582337601847	1.0185220439754818
7	0.18529437899303722	0.7709524902404572
8	0.14613838156904174	0.6948988788396867
9	0.10802472169132796	0.5778737887497385
>10	0.7757635775573022	9.498679948464057
>50	0.13404384556369892	5.584718120812577
>100	0.10008851970847585	10.816886742301083
>500	0.003919128067776668	1.4455246125057024
>1k	6.029427796579489E-4	0.9390444690512126
>5k	3.014713898289744E-4	1.1478006576676925
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6699	0.5988698443689339	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6112	0.5463938630814933	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4889	0.43706145232418536	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3517	0.3144089032162323	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.93969016821815E-5	0.0
2	0.0	0.0	0.0	0.001072762820186178	0.0
3	0.0	0.0	0.0	0.001609144230279267	0.0
4	0.0	0.0	0.0	0.003397082263922897	0.0
5	0.0	0.0	0.0	0.003754669870651623	0.0
6	0.0	0.0	0.0	0.003754669870651623	0.0
7	0.0	0.0	0.0	0.004380448182426893	0.0
8	0.0	0.0	0.0	0.005006226494202164	0.0
9	0.0	0.0	0.0	0.008403308758125061	0.0
10	0.0	0.0	0.0	0.01197918482541232	0.0
11	0.0	0.0	0.0	0.013409535252327225	0.0
12	0.0	0.0	0.0	0.01430350426914904	0.0
13	0.0	0.0	0.0	0.015197473285970854	0.0
14	0.0	0.0	0.0	0.016627823712885757	0.0
15	0.0	0.0	0.0	0.018773349353258115	0.0
16	0.0	0.0	0.0	0.022706813027274102	0.0
17	0.0	0.0	0.0	0.027891833324840628	0.0
18	0.0	8.93969016821815E-5	0.0	0.02932218375175553	0.0
19	0.0	8.93969016821815E-5	0.0	0.03271926601567843	0.0
20	0.0	8.93969016821815E-5	0.0	0.036295142082965685	0.0
21	0.0	8.93969016821815E-5	0.0	0.04228473449567185	0.0
22	0.0	8.93969016821815E-5	0.0	0.05202899677902963	0.0
23	0.0	8.93969016821815E-5	0.0	0.06686888245827176	0.0
24	0.0	8.93969016821815E-5	0.0	0.08939690168218149	0.0
25	0.0	8.93969016821815E-5	0.0	0.09645925691507384	0.0
26	0.0	8.93969016821815E-5	0.0	0.11031577667581197	0.0
27	0.0	8.93969016821815E-5	0.0	0.125602646863465	0.0
28	0.0	8.93969016821815E-5	0.0	0.15966286640437616	0.0
29	0.0	8.93969016821815E-5	0.0	0.20749020880434327	0.0
30	0.0	8.93969016821815E-5	0.0	0.27891833324840626	0.0
31	0.0	8.93969016821815E-5	0.0	0.5138533908691792	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTCA	100	2.874749E-7	18.5	3
TAATACT	180	0.0	18.5	4
AAGACGG	265	0.0	18.150944	5
CTAGCGG	185	1.8189894E-12	17.000002	29
GCGCAAG	285	0.0	16.877193	1
CGCAAGA	285	0.0	16.877193	2
ACGGACC	265	0.0	16.754717	8
CGGACCA	265	0.0	16.754717	9
CAAGACG	300	0.0	16.65	4
TCTAGCG	180	1.0913936E-11	16.444445	28
GACGGAC	275	0.0	16.145454	7
TTAATCG	115	1.242046E-6	16.086956	37
CCGGTCG	165	9.731593E-10	15.696971	20
TGGACTA	95	7.059403E-5	15.578948	5
TCGCCAT	215	0.0	15.488372	13
AACGCCG	180	2.0190782E-10	15.416666	5
CGTCGTA	180	2.0190782E-10	15.416666	10
TAACGCC	170	1.4861143E-9	15.235294	4
AATAACG	170	1.4861143E-9	15.235294	2
TGCGTAC	85	5.363991E-4	15.235294	11
>>END_MODULE