FastQCFastQC Report
Fri 10 Feb 2017
ERR1630526.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630526.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87676
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT26453.0167890870933896No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22142.5252064418997215No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15021.7131255987955656No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT14761.683470961266481TruSeq Adapter, Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT13371.5249327067840686No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11711.335599251790684No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC7890.8999041927095214No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC7420.8462977325607921No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG7390.8428760436151284No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3400.3877914138418724No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3370.38436972489620874No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC3330.3798074729686573RNA PCR Primer, Index 11 (95% over 22bp)
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2770.31593594598293717No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2320.26461061179798345No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT2270.2589077968885442No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG2090.23837766321456272No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA2090.23837766321456272No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1930.22012865550435695No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1880.21442584059491765No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1770.2018796477941512No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA1540.17564669921073042No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1520.1733655732469547No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1510.17222501026506684No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1360.15511656553674893No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.1368675578265432No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA1150.13116474291710387No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1130.12888361695332815No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA1110.12660249098955245No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1050.11975911309822529No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1040.11861855011633743No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA1000.11405629818878597No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA1000.11405629818878597No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA980.11177517222501025No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT980.11177517222501025No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA930.10607235731557096No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC352.3634128E-531.71428537
CCGTCTT2500.031.0837
GCCGTCT2500.031.0836
GCGAGAC250.00546902929.629
CACTGTC358.803925E-426.4285735
TGCCGTC3100.025.06451635
TATACAC2050.024.3658523
ATGCCGT3250.023.90769434
AACCGAG400.001917066623.12500229
GTACCCC400.001917066623.1250028
AAGAAAT400.001917066623.12500221
TACCCCG400.001917066623.1250029
TATGCCG3450.023.05797233
TTATACA2300.021.717392
TACACAT2400.020.8124985
GAGGAAA450.003798102320.55555315
CAACCGA450.003798102320.55555328
AGGAAAA450.003798102320.55555316
TCAACCG450.003798102320.55555327
ACCGAGA450.003798102320.55555330
Produced by FastQC (version 0.11.5)