FastQCFastQC Report
Fri 10 Feb 2017
ERR1630525.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630525.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1744576
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT248121.4222366924685426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT210461.2063676217029238No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT172120.9866007557137093No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132180.7576626068454455No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36390.20858936498037345No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34460.19752650500752042No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT31880.18273781136505374No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27310.1565423346417697No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA26990.15470807806595988No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC21610.12386963938515719No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG21320.12220734436332954No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT20770.11905471587365642No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19860.11383854873619721No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC18860.10810649693679152No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG17670.10128535529549873No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA17580.10076947063355221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA1155.456968E-1222.521743
ATGTACG705.1044226E-621.14285911
TTTAGCG1059.840733E-1021.14285726
GCAGCGT1551.8189894E-1219.0967751
CGAATTG500.00703630918.49999814
GGTATCA107250.018.042891
CAGCGTC1552.1827873E-1117.9032272
ACTCGCA1156.4104825E-817.69565221
TCGGGTC953.608553E-617.52631637
CTATATC2050.017.1463412
TAGCGGG1301.3944373E-817.07692328
CCGAATT650.001580377517.07692313
TCCGTAT1201.04208084E-716.95833213
GCGAAAG1801.0913936E-1116.44444518
GTACGTA904.448081E-516.44444513
GTAAACG1251.6590457E-716.27999927
ACGGACC1606.2937033E-1016.18758
TCTATCG1606.2937033E-1016.187529
TCTAGCA2750.016.1454543
CGCAAGA1851.8189894E-1116.02
Produced by FastQC (version 0.11.5)