##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630525.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1744576
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00366736674126	33.0	31.0	34.0	30.0	34.0
2	32.13665154169265	33.0	31.0	34.0	30.0	34.0
3	32.21228309915991	34.0	31.0	34.0	30.0	34.0
4	35.8132314098096	37.0	35.0	37.0	35.0	37.0
5	35.81859890311457	37.0	35.0	37.0	35.0	37.0
6	35.885723522506325	37.0	35.0	37.0	35.0	37.0
7	35.85880122161488	37.0	35.0	37.0	35.0	37.0
8	35.88301455482593	37.0	35.0	37.0	35.0	37.0
9	37.565892801460066	39.0	37.0	39.0	35.0	39.0
10	37.493163381818846	39.0	37.0	39.0	35.0	39.0
11	37.62250598426208	39.0	37.0	39.0	35.0	39.0
12	37.571259721559855	39.0	37.0	39.0	35.0	39.0
13	37.61975116016728	39.0	37.0	39.0	35.0	39.0
14	38.83700394823728	40.0	38.0	41.0	35.0	41.0
15	38.795173153820755	40.0	38.0	41.0	35.0	41.0
16	38.734882286584245	40.0	38.0	41.0	35.0	41.0
17	38.798761991452366	40.0	38.0	41.0	35.0	41.0
18	38.82258382552551	40.0	38.0	41.0	35.0	41.0
19	38.88826110183793	40.0	38.0	41.0	35.0	41.0
20	38.87283615044572	40.0	38.0	41.0	35.0	41.0
21	38.80669171191166	40.0	38.0	41.0	35.0	41.0
22	38.71043050001834	40.0	38.0	41.0	35.0	41.0
23	38.66241252888954	40.0	38.0	41.0	35.0	41.0
24	38.59972910323196	40.0	38.0	41.0	34.0	41.0
25	38.52479284364797	40.0	38.0	41.0	34.0	41.0
26	38.28894585274588	40.0	37.0	41.0	34.0	41.0
27	38.12167598316152	40.0	37.0	41.0	34.0	41.0
28	37.9957450979493	40.0	37.0	41.0	33.0	41.0
29	37.86393140797535	40.0	37.0	41.0	33.0	41.0
30	37.70336058732896	40.0	36.0	41.0	33.0	41.0
31	37.56763649161745	40.0	36.0	41.0	33.0	41.0
32	37.365601727869695	40.0	35.0	41.0	33.0	41.0
33	37.2455049249789	39.0	35.0	41.0	33.0	41.0
34	37.10320100700686	39.0	35.0	41.0	32.0	41.0
35	36.94772483399978	39.0	35.0	41.0	31.0	41.0
36	36.82253739590594	39.0	35.0	41.0	31.0	41.0
37	36.53268759859129	39.0	35.0	41.0	31.0	41.0
38	36.319475333834696	39.0	35.0	41.0	30.0	41.0
39	36.119932866209325	39.0	35.0	41.0	30.0	41.0
40	35.88573899904619	39.0	35.0	41.0	28.0	41.0
41	35.62346438332294	39.0	35.0	41.0	26.0	41.0
42	35.37760292472211	39.0	35.0	41.0	24.0	41.0
43	34.53008180784328	38.0	34.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	11.0
11	8.0
12	8.0
13	9.0
14	11.0
15	7.0
16	10.0
17	27.0
18	50.0
19	138.0
20	315.0
21	600.0
22	1119.0
23	1911.0
24	3159.0
25	5131.0
26	7790.0
27	11734.0
28	16699.0
29	23478.0
30	30377.0
31	38447.0
32	48489.0
33	62368.0
34	82005.0
35	106082.0
36	149212.0
37	244198.0
38	369045.0
39	542129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.4744877838512	20.756676693935947	12.882671778128325	20.886163744084524
2	18.712111137605927	22.347378388789025	34.62119162478447	24.319318848820572
3	22.11196302138743	25.97823196008658	28.980680692615284	22.929124325910706
4	13.51726723284053	16.598932921237022	34.29326094134048	35.590538904581976
5	12.428463993543417	40.5246890935104	33.17831954583807	13.868527367108111
6	31.848426207857955	34.32071746945963	17.01272974063612	16.818126582046297
7	26.790635661616346	33.01615980043289	22.001792985802854	18.191411552147915
8	28.95505842107194	31.694749899115887	20.322473770130966	19.027717909681204
9	27.116502806412562	12.789182013280017	22.778543325140323	37.3157718551671
10	19.37519488976118	28.182377838512053	31.239453024689094	21.202974247037677
11	32.0448063025056	23.52004154591144	21.047692963791775	23.387459187791187
12	21.322602168091272	29.70062639862064	28.120872922704425	20.85589851058366
13	29.001602681683114	21.002925639238416	28.290255053376868	21.705216625701603
14	22.429289409002532	22.971082945082358	28.514894163395578	26.084733482519535
15	26.425962526138157	26.747358670530836	26.4612146447045	20.36546415862651
16	23.11128893209582	26.53859734399648	28.385235151693017	21.964878572214683
17	21.13281393301295	28.299827579881875	28.10757456252981	22.45978392457537
18	20.190521937708645	24.993408140430684	31.54181875710774	23.274251164752926
19	21.045686745661985	26.359413404747055	32.17778990425181	20.417109945339153
20	21.973820573021754	24.686399446054512	32.81066574342419	20.52911423749954
21	22.896336989618106	25.767579056458416	31.13088796360835	20.205195990315126
22	23.151069371583695	25.609832990938774	30.844514655710043	20.39458298176749
23	21.775892824388276	25.94464213654206	31.32744001980997	20.952025019259693
24	20.46921429619575	27.565895667485968	30.67381415312374	21.29107588319454
25	20.958559558311016	26.804679188524894	31.40075296232437	20.83600829083972
26	20.831250687846218	27.653939983124843	30.966148794893428	20.548660534135514
27	21.152532191202905	27.163448310649695	30.830929692945446	20.853089805201954
28	20.0067523570197	26.943910726732454	32.113705565134445	20.935631351113393
29	20.30625206353865	27.26725576873693	32.22186938258923	20.204622785135186
30	19.27700484243736	28.31278201694853	32.003478209031876	20.40673493158223
31	20.55525239370483	28.57072434792179	30.398503705198284	20.475519553175097
32	19.371469056091566	27.98691487215232	31.326350929968083	21.315265141788036
33	19.12309925162332	27.675205895300635	31.692170475806158	21.50952437726989
34	20.472940129865368	27.178294324810153	31.6618479217873	20.68691762353718
35	19.413141072673245	27.826360101250962	31.500318702080047	21.260180123995745
36	19.55082495689497	28.486004622326572	30.3105740305954	21.65259639018306
37	19.851872317399756	27.167002182765327	31.362405535786348	21.618719964048573
38	19.518553505264315	26.677771561686047	31.471830404637004	22.331844528412635
39	19.382359954510438	27.410614384240066	31.83335091162552	21.373674749623977
40	18.779978630910893	27.148487655453245	32.106712461939175	21.964821251696687
41	18.559925162331705	27.15656984849041	32.09020415275688	22.193300836421
42	18.416910469936536	27.56549442386001	32.30870996735023	21.708885138853223
43	18.241165761766755	26.415644832899225	31.793169228511687	23.550020176822333
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2344.0
1	1709.5
2	1075.0
3	2661.5
4	4248.0
5	4248.0
6	5958.0
7	7668.0
8	7646.5
9	7625.0
10	11195.0
11	14765.0
12	14765.0
13	25398.5
14	36032.0
15	44998.5
16	53965.0
17	48665.5
18	43366.0
19	43366.0
20	47485.0
21	51604.0
22	38816.0
23	26028.0
24	25203.5
25	24379.0
26	24379.0
27	26419.0
28	28459.0
29	32084.0
30	35709.0
31	41565.5
32	47422.0
33	47422.0
34	56361.5
35	65301.0
36	70741.5
37	76182.0
38	86036.5
39	95891.0
40	95891.0
41	102643.0
42	109395.0
43	115374.5
44	121354.0
45	120078.0
46	118802.0
47	118802.0
48	122675.0
49	126548.0
50	122042.5
51	117537.0
52	114620.5
53	111704.0
54	111704.0
55	103575.5
56	95447.0
57	86500.5
58	77554.0
59	71931.0
60	66308.0
61	66308.0
62	61587.5
63	56867.0
64	48774.0
65	40681.0
66	36028.5
67	31376.0
68	31376.0
69	25445.5
70	19515.0
71	16306.5
72	13098.0
73	9537.5
74	5977.0
75	5977.0
76	4681.0
77	3385.0
78	3029.5
79	2674.0
80	2093.0
81	1512.0
82	1512.0
83	1225.0
84	938.0
85	873.0
86	808.0
87	689.5
88	571.0
89	571.0
90	475.0
91	379.0
92	220.0
93	61.0
94	38.5
95	16.0
96	16.0
97	11.0
98	6.0
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1744576.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.4918534534054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.174725402958	32.86915085137
2	10.612604781807745	8.594480751677372
3	3.0476221402540715	3.7021160725356466
4	1.243246690555743	2.0136545120165756
5	0.6703179186709376	1.3571207465007662
6	0.4575122897717677	1.1115312354342217
7	0.3160431333579073	0.8958020568618408
8	0.25226725397767974	0.8171814943325766
9	0.19458589044053673	0.7091229023836749
>10	1.5846629602561435	13.17631703720533
>50	0.23842481422755474	6.756795835230135
>100	0.1831079808139759	14.273187762067376
>500	0.01677538093224097	4.614232721227334
>1k	0.007534704994989589	4.717792044374258
>5k	0.0	0.0
>10k+	5.686569807539312E-4	4.391513976782949
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	24812	1.4222366924685426	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	21046	1.2063676217029238	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	17212	0.9866007557137093	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13218	0.7576626068454455	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3639	0.20858936498037345	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3446	0.19752650500752042	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3188	0.18273781136505374	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2731	0.1565423346417697	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2699	0.15470807806595988	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2161	0.12386963938515719	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	2132	0.12220734436332954	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	2077	0.11905471587365642	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1986	0.11383854873619721	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1886	0.10810649693679152	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1767	0.10128535529549873	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	1758	0.10076947063355221	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.7196155398217102E-4	0.0
3	0.0	0.0	0.0	4.0124362595839907E-4	0.0
4	0.0	0.0	0.0	5.732051799405701E-4	0.0
5	0.0	0.0	0.0	6.305256979346271E-4	0.0
6	0.0	0.0	0.0	6.305256979346271E-4	0.0
7	0.0	0.0	0.0	6.305256979346271E-4	0.0
8	0.0	0.0	0.0	6.878462159286841E-4	0.0
9	1.1464103598811402E-4	0.0	0.0	0.0010317693238930262	0.0
10	1.1464103598811402E-4	0.0	0.0	0.0015476539858395393	0.0
11	1.1464103598811402E-4	0.0	0.0	0.0023501412377563372	0.0
12	1.1464103598811402E-4	0.0	0.0	0.005044205583477017	0.0
13	1.7196155398217102E-4	0.0	0.0	0.006362577497340328	0.0
14	1.7196155398217102E-4	0.0	0.0	0.008483436663120437	0.0
15	1.7196155398217102E-4	0.0	0.0	0.012495872922704428	0.0
16	1.7196155398217102E-4	0.0	0.0	0.021724476319747606	0.0
17	1.7196155398217102E-4	0.0	0.0	0.03674245203419054	0.0
18	1.7196155398217102E-4	0.0	0.0	0.040926849847756706	0.0
19	1.7196155398217102E-4	0.0	0.0	0.050270094280788	0.0
20	1.7196155398217102E-4	0.0	0.0	0.055256979346270954	0.0
21	1.7196155398217102E-4	0.0	0.0	0.07130672438460692	0.0
22	1.7196155398217102E-4	0.0	0.0	0.10243176565537987	0.0
23	1.7196155398217102E-4	0.0	0.0	0.14593803881286915	0.0
24	2.2928207197622804E-4	0.0	0.0	0.22498303312667375	0.0
25	2.2928207197622804E-4	0.0	0.0	0.24217918852489087	0.0
26	2.8660258997028503E-4	0.0	0.0	0.27290298616970543	0.0
27	2.8660258997028503E-4	0.0	0.0	0.3111930921897355	0.0
28	2.8660258997028503E-4	0.0	0.0	0.3818692908764078	0.0
29	2.8660258997028503E-4	0.0	0.0	0.4767920686745662	0.0
30	2.8660258997028503E-4	0.0	0.0	0.6461741993470047	0.0
31	2.8660258997028503E-4	0.0	0.0	1.149219065262849	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTCA	115	5.456968E-12	22.52174	3
ATGTACG	70	5.1044226E-6	21.142859	11
TTTAGCG	105	9.840733E-10	21.142857	26
GCAGCGT	155	1.8189894E-12	19.096775	1
CGAATTG	50	0.007036309	18.499998	14
GGTATCA	10725	0.0	18.04289	1
CAGCGTC	155	2.1827873E-11	17.903227	2
ACTCGCA	115	6.4104825E-8	17.695652	21
TCGGGTC	95	3.608553E-6	17.526316	37
CTATATC	205	0.0	17.146341	2
TAGCGGG	130	1.3944373E-8	17.076923	28
CCGAATT	65	0.0015803775	17.076923	13
TCCGTAT	120	1.04208084E-7	16.958332	13
GCGAAAG	180	1.0913936E-11	16.444445	18
GTACGTA	90	4.448081E-5	16.444445	13
GTAAACG	125	1.6590457E-7	16.279999	27
ACGGACC	160	6.2937033E-10	16.1875	8
TCTATCG	160	6.2937033E-10	16.1875	29
TCTAGCA	275	0.0	16.145454	3
CGCAAGA	185	1.8189894E-11	16.0	2
>>END_MODULE