FastQCFastQC Report
Fri 10 Feb 2017
ERR1630524.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630524.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210279
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT65983.137736055431118No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT62622.977948344818075No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT56492.68643088468178No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38471.8294741747868308No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT37521.7842961018456431TruSeq Adapter, Index 1 (95% over 22bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28441.3524888362603968No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG23861.1346829688176185No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC22271.0590691414739466No Hit
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC17490.8317521007803916No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC8910.4237227683220864RNA PCR Primer, Index 1 (95% over 23bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6760.3214776558762406No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6460.30721089600007606No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6130.2915174601362951No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6110.29056634281121746No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG5410.25727723643350026No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA4880.23207262731894293No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA4750.22589036470593832No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA4640.2206592194180113No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4400.20924581151707972No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA4320.20544134221676916No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT4180.1987835209412257No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4080.19402793431583754No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA3740.17785893978951775No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA3440.16359217991335323No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3390.16121438660065912No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2990.14219204009910644No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2760.13125419086071363No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2700.1284008388854807No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA2570.12221857627247609No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA2460.11698743098454911No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC2360.11223184435916092No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.10557402308361748No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA2220.10557402308361748No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA2210.10509846442107865No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT2190.10414734709600104No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT2140.10176955378330695No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC2110.10034287779569048No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTGC200.001838518137.08
CGTCTTC1100.030.27272837
AACCGCG250.0054862629.67
GGAACGC250.0054862629.68
TTAGTCG250.0054862629.69
GCCGTCT5200.028.46153836
TATACAC8500.028.2941193
CCGTCTT5250.028.19047537
TTATACA8800.027.3295462
GACCGTT358.845213E-426.428577
GGACCGT358.845213E-426.428576
CTAGCAC358.845213E-426.428573
ATGCCCC509.045041E-625.90000237
CTTATAC9700.024.7938141
CAGTGGC701.9074105E-723.78571326
GTGCGCC400.001925971923.12511
CCGCTTA1155.456968E-1222.52173825
TACACAT10600.022.514155
ATACACA10750.022.3720914
AGTGGCT753.7107748E-722.227
Produced by FastQC (version 0.11.5)