##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630524.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	210279
Sequences flagged as poor quality	0
Sequence length	43
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.78172332948131	31.0	31.0	34.0	30.0	34.0
2	31.857632003195754	33.0	31.0	34.0	30.0	34.0
3	31.833901625935066	33.0	31.0	34.0	30.0	34.0
4	35.5583676924467	37.0	35.0	37.0	33.0	37.0
5	35.60513413132077	37.0	35.0	37.0	33.0	37.0
6	35.68697302155707	37.0	35.0	37.0	33.0	37.0
7	35.62088938981068	37.0	35.0	37.0	33.0	37.0
8	35.70618559152364	37.0	35.0	37.0	33.0	37.0
9	37.29555495318125	39.0	37.0	39.0	34.0	39.0
10	37.2211062445608	39.0	37.0	39.0	34.0	39.0
11	37.383923263854214	39.0	37.0	39.0	34.0	39.0
12	37.31844834719587	39.0	37.0	39.0	34.0	39.0
13	37.42134021942277	39.0	37.0	39.0	34.0	39.0
14	38.39873216060567	40.0	38.0	41.0	34.0	41.0
15	38.338816524712406	40.0	38.0	41.0	34.0	41.0
16	38.183028262451316	40.0	37.0	41.0	33.0	41.0
17	38.327640896142746	40.0	38.0	41.0	34.0	41.0
18	38.476210177906495	40.0	38.0	41.0	34.0	41.0
19	38.546183879512455	40.0	38.0	41.0	34.0	41.0
20	38.61143052801278	40.0	38.0	41.0	34.0	41.0
21	38.54020610712434	40.0	38.0	41.0	34.0	41.0
22	38.40803408804493	40.0	38.0	41.0	34.0	41.0
23	38.34807565187204	40.0	38.0	41.0	34.0	41.0
24	38.22595218733207	40.0	37.0	41.0	34.0	41.0
25	38.12148146034554	40.0	37.0	41.0	33.0	41.0
26	37.76319080840217	40.0	37.0	41.0	33.0	41.0
27	37.4597463370094	39.0	35.0	41.0	33.0	41.0
28	37.34076155964219	39.0	35.0	41.0	32.0	41.0
29	37.079684609495004	39.0	35.0	41.0	32.0	41.0
30	36.82668740102435	39.0	35.0	41.0	31.0	41.0
31	36.50686944488037	38.0	35.0	40.0	31.0	41.0
32	36.14124092277403	38.0	35.0	40.0	30.0	41.0
33	35.85396069032095	38.0	35.0	40.0	30.0	41.0
34	35.58467559765835	38.0	35.0	40.0	30.0	41.0
35	35.31537623823587	38.0	35.0	40.0	28.0	41.0
36	35.02455309374688	37.0	35.0	40.0	26.0	41.0
37	34.51934334859877	37.0	34.0	40.0	23.0	41.0
38	34.14638171191607	36.0	33.0	40.0	21.0	41.0
39	33.74092039623548	36.0	33.0	40.0	18.0	41.0
40	33.247499750331706	35.0	33.0	40.0	15.0	41.0
41	32.788928043218775	35.0	33.0	40.0	12.0	41.0
42	32.3090322856776	35.0	32.0	40.0	10.0	41.0
43	31.26414430352056	35.0	30.0	39.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	4.0
10	2.0
11	3.0
12	3.0
13	2.0
14	1.0
15	2.0
16	1.0
17	2.0
18	9.0
19	29.0
20	64.0
21	157.0
22	256.0
23	430.0
24	773.0
25	1148.0
26	1803.0
27	2606.0
28	3527.0
29	4613.0
30	5777.0
31	6868.0
32	8097.0
33	9925.0
34	12400.0
35	15419.0
36	20784.0
37	35330.0
38	40293.0
39	39950.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.454848082785254	19.473176113639497	15.001498009786998	20.070477793788253
2	17.314615344375806	23.4940246054052	33.94442621469572	25.246933835523283
3	21.935618868265493	26.802961779350294	33.78891853204552	17.472500820338695
4	12.369280812634642	19.495051812116284	26.96988286990142	41.16578450534766
5	11.119037088820091	42.160653227378866	31.428720889865367	15.291588793935675
6	29.583553279214758	30.204157333827915	18.776007114357594	21.436282272599737
7	23.346125861355628	35.98124396634947	19.29151270454967	21.381117467745234
8	34.26970833987226	29.348627299920583	19.814627233342367	16.567037126864783
9	26.12671736122009	10.272067110838458	21.16568939361515	42.4355261343263
10	20.764793441094927	30.974562367140802	24.541680338978214	23.71896385278606
11	27.871066535412474	23.11595546868684	19.835076255831538	29.177901740069146
12	18.854474293676496	33.460307496231195	25.673034397158062	22.012183812934243
13	32.94860637533943	17.09585835960795	29.755229956391272	20.200305308661353
14	23.266232006049105	23.816928937269058	29.004798386905016	23.91204066977682
15	29.628731352155945	23.5896118965755	25.623576296254026	21.158080455014527
16	24.146966649070997	23.017514825541305	30.494248117976593	22.341270407411105
17	16.71826478155213	34.29538850764936	28.546359836217594	20.439986874580914
18	19.76088910447548	20.962625844711074	33.02517132000818	26.251313730805265
19	18.139709623880655	29.271111237926757	35.098607088677426	17.49057204951517
20	18.375111161837367	21.874271800797988	38.6633948230684	21.087222214296244
21	23.47785561087888	22.107771104104547	34.03620903656571	20.378164248450865
22	20.358666343286778	26.815326304576303	35.08909591542665	17.736911436710276
23	19.31196172703884	24.16503787824747	32.90961056501125	23.613389829702445
24	19.64485279081601	29.24495551148712	34.90172580238635	16.208465895310518
25	18.31091074239463	27.670380779821098	34.33818878727786	19.680519690506422
26	22.153424735708274	26.807241807313144	33.220625930311634	17.81870752666695
27	17.07921380641909	30.058636383091038	35.42864480047936	17.43350501001051
28	16.611739641143433	25.96692965060705	35.867109887340156	21.554220820909364
29	16.00254899443121	30.79717898601382	35.99883963686341	17.201432382691568
30	16.143789917205236	27.30610284431636	40.02967486054242	16.520432377935983
31	16.646455423508765	28.840730648329128	33.06559380632398	21.44722012183813
32	16.87044355356455	29.24019992486173	37.960994678498565	15.928361843075153
33	15.638271058926472	27.85347086489854	36.98800165494415	19.52025642123084
34	15.886512680771736	33.82220763842324	32.63473765806381	17.656542022741213
35	17.870067862221145	28.69806304956748	36.888134335810996	16.543734752400383
36	18.83925641647525	29.30535146162955	31.764465305617772	20.09092681627742
37	15.483714493601358	31.45344994031739	33.8621545660765	19.200681000004753
38	15.616395360449689	27.58478022056411	35.78531379738348	21.013510621602727
39	17.897174705985858	28.180179666062706	33.1859101479463	20.736735480005137
40	14.811274544771472	27.472072817542408	38.65198141516747	19.064671222518655
41	15.28968655928552	29.348627299920583	32.5928884957604	22.768797645033505
42	16.213221481935904	27.85347086489854	36.17099187270246	19.762315780463098
43	14.267710993489601	28.884482045282695	36.057333352355684	20.790473608872023
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	33.0
1	56.0
2	79.0
3	353.5
4	628.0
5	628.0
6	972.5
7	1317.0
8	1397.0
9	1477.0
10	2100.5
11	2724.0
12	2724.0
13	5052.0
14	7380.0
15	10615.5
16	13851.0
17	11949.0
18	10047.0
19	10047.0
20	11488.5
21	12930.0
22	9051.5
23	5173.0
24	4715.5
25	4258.0
26	4258.0
27	4363.5
28	4469.0
29	4354.5
30	4240.0
31	4266.5
32	4293.0
33	4293.0
34	4230.0
35	4167.0
36	4192.5
37	4218.0
38	4635.0
39	5052.0
40	5052.0
41	5718.5
42	6385.0
43	7205.0
44	8025.0
45	9640.0
46	11255.0
47	11255.0
48	13119.0
49	14983.0
50	17499.0
51	20015.0
52	14441.5
53	8868.0
54	8868.0
55	11425.0
56	13982.0
57	12187.5
58	10393.0
59	8348.0
60	6303.0
61	6303.0
62	5935.0
63	5567.0
64	5166.0
65	4765.0
66	4474.5
67	4184.0
68	4184.0
69	3787.5
70	3391.0
71	2886.5
72	2382.0
73	1965.5
74	1549.0
75	1549.0
76	1235.0
77	921.0
78	717.5
79	514.0
80	402.5
81	291.0
82	291.0
83	203.0
84	115.0
85	75.0
86	35.0
87	24.5
88	14.0
89	14.0
90	10.0
91	6.0
92	3.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	210279.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.89997099092159
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.03547524814024	37.65425934116103
2	9.618931183820635	8.830173246020763
3	3.1393108021301726	4.3228282424778515
4	1.3790173853581715	2.5318743193566644
5	0.8568349945087962	1.9664350695980104
6	0.5667336662591433	1.560783530452399
7	0.39578109782631216	1.2716438636287979
8	0.28181271887109144	1.034815649684467
9	0.23311713877204251	0.9630062916411055
>10	1.293023063055596	11.207491000052311
>50	0.12122091216146211	3.96520812824866
>100	0.06320064651153152	6.005830349202726
>500	0.006216457033921133	1.8917723595794158
>1k	0.006216457033921133	7.991763323964827
>5k	0.0031082285169605666	8.802115284930972
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6598	3.137736055431118	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6262	2.977948344818075	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT	5649	2.68643088468178	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3847	1.8294741747868308	No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT	3752	1.7842961018456431	TruSeq Adapter, Index 1 (95% over 22bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2844	1.3524888362603968	No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG	2386	1.1346829688176185	No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC	2227	1.0590691414739466	No Hit
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC	1749	0.8317521007803916	No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC	891	0.4237227683220864	RNA PCR Primer, Index 1 (95% over 23bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	676	0.3214776558762406	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	646	0.30721089600007606	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	613	0.2915174601362951	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	611	0.29056634281121746	No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG	541	0.25727723643350026	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	488	0.23207262731894293	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	475	0.22589036470593832	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	464	0.2206592194180113	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	440	0.20924581151707972	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	432	0.20544134221676916	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	418	0.1987835209412257	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	408	0.19402793431583754	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	374	0.17785893978951775	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	344	0.16359217991335323	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	339	0.16121438660065912	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	299	0.14219204009910644	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	276	0.13125419086071363	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	270	0.1284008388854807	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	257	0.12221857627247609	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	246	0.11698743098454911	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	236	0.11223184435916092	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	222	0.10557402308361748	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	222	0.10557402308361748	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	221	0.10509846442107865	No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT	219	0.10414734709600104	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	214	0.10176955378330695	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	211	0.10034287779569048	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0014266759876164525	0.0
3	0.0	0.0	0.0	0.002377793312694087	0.0
4	0.0	0.0	0.0	0.002377793312694087	0.0
5	0.0	0.0	0.0	0.002853351975232905	0.0
6	0.0	0.0	0.0	0.002853351975232905	0.0
7	0.0	0.0	0.0	0.002853351975232905	0.0
8	0.0	0.0	0.0	0.003328910637771722	0.0
9	0.0	0.0	0.0	0.004755586625388174	0.0
10	4.755586625388175E-4	0.0	0.0	0.00570670395046581	0.0
11	4.755586625388175E-4	0.0	0.0	0.0071333799380822625	0.0
12	4.755586625388175E-4	0.0	0.0	0.008560055925698714	0.0
13	4.755586625388175E-4	0.0	0.0	0.008560055925698714	0.0
14	9.51117325077635E-4	0.0	0.0	0.012364525226009254	0.0
15	9.51117325077635E-4	0.0	0.0	0.016168994526319796	0.0
16	9.51117325077635E-4	0.0	0.0	0.036618017015488946	0.0
17	9.51117325077635E-4	0.0	0.0	0.06182262613004627	0.0
18	9.51117325077635E-4	0.0	0.0	0.06943156473066735	0.0
19	9.51117325077635E-4	0.0	0.0	0.08750279390714241	0.0
20	9.51117325077635E-4	0.0	0.0	0.09416061518268586	0.0
21	9.51117325077635E-4	0.0	0.0	0.11746298964708791	0.0
22	9.51117325077635E-4	0.0	0.0	0.17643226380190127	4.755586625388175E-4
23	9.51117325077635E-4	0.0	0.0	0.2539483257957285	4.755586625388175E-4
24	0.0014266759876164525	0.0	0.0	0.37141131544281647	4.755586625388175E-4
25	0.0014266759876164525	0.0	0.0	0.3970914832199126	4.755586625388175E-4
26	0.0014266759876164525	0.0	0.0	0.4470251427864884	4.755586625388175E-4
27	0.0014266759876164525	0.0	0.0	0.4817409251518221	4.755586625388175E-4
28	0.0014266759876164525	0.0	0.0	0.5426124339567907	4.755586625388175E-4
29	0.0014266759876164525	0.0	0.0	0.6557953956410293	4.755586625388175E-4
30	0.0014266759876164525	0.0	0.0	0.8512500059444833	4.755586625388175E-4
31	0.0014266759876164525	0.0	0.0	1.3529643949229357	4.755586625388175E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGTGC	20	0.0018385181	37.0	8
CGTCTTC	110	0.0	30.272728	37
AACCGCG	25	0.00548626	29.6	7
GGAACGC	25	0.00548626	29.6	8
TTAGTCG	25	0.00548626	29.6	9
GCCGTCT	520	0.0	28.461538	36
TATACAC	850	0.0	28.294119	3
CCGTCTT	525	0.0	28.190475	37
TTATACA	880	0.0	27.329546	2
GACCGTT	35	8.845213E-4	26.42857	7
GGACCGT	35	8.845213E-4	26.42857	6
CTAGCAC	35	8.845213E-4	26.42857	3
ATGCCCC	50	9.045041E-6	25.900002	37
CTTATAC	970	0.0	24.793814	1
CAGTGGC	70	1.9074105E-7	23.785713	26
GTGCGCC	40	0.0019259719	23.125	11
CCGCTTA	115	5.456968E-12	22.521738	25
TACACAT	1060	0.0	22.51415	5
ATACACA	1075	0.0	22.372091	4
AGTGGCT	75	3.7107748E-7	22.2	27
>>END_MODULE