FastQCFastQC Report
Fri 10 Feb 2017
ERR1630523.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630523.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2139584
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT89200.41690347282462387No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT83310.38937475696210105No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT65630.3067418713170411No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52390.2448606831982292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41300.018.8583551
TCCGTGA650.001580535117.0769238
AAGACGG3850.015.8571425
ATCTCGC2350.015.74468111
CGAATTA1557.21775E-915.516129515
GAACGTT1455.3520125E-815.31034511
GACGGAC3900.015.1794877
ATACCGT2201.8189894E-1215.1363646
TGTACCG1853.0559022E-1015.05
CGGACCA3850.014.8961049
TACGAGT750.00410594214.84
TACCGTC2251.8189894E-1214.87
ACGGACC4150.014.7108438
GCGCAAG4300.014.6279071
CCAATAC2301.8189894E-1214.4782613
GTCTTAG3350.014.3582091
TACGGGG4350.014.0344832
TGCGTAC1458.9195237E-714.03448316
ATCCGTG800.006300960713.8757
AGACGGA4400.013.8756
Produced by FastQC (version 0.11.5)