##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630523.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2139584
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.94417980317669	33.0	31.0	34.0	30.0	34.0
2	32.113871668511266	33.0	31.0	34.0	30.0	34.0
3	32.21670661212647	34.0	31.0	34.0	30.0	34.0
4	35.80550144327122	37.0	35.0	37.0	35.0	37.0
5	35.78183142143519	37.0	35.0	37.0	35.0	37.0
6	35.82102034788071	37.0	35.0	37.0	35.0	37.0
7	35.79856504815889	37.0	35.0	37.0	35.0	37.0
8	35.79309108686549	37.0	35.0	37.0	35.0	37.0
9	37.50836143848524	39.0	37.0	39.0	35.0	39.0
10	37.40885237504113	39.0	37.0	39.0	34.0	39.0
11	37.52240856166432	39.0	37.0	39.0	35.0	39.0
12	37.456629419550715	39.0	37.0	39.0	35.0	39.0
13	37.50612922885944	39.0	37.0	39.0	35.0	39.0
14	38.767156606144	40.0	38.0	41.0	35.0	41.0
15	38.738371103915526	40.0	38.0	41.0	35.0	41.0
16	38.71549609643744	40.0	38.0	41.0	35.0	41.0
17	38.71086996350692	40.0	38.0	41.0	35.0	41.0
18	38.70296328632108	40.0	38.0	41.0	35.0	41.0
19	38.74078746148784	40.0	38.0	41.0	34.0	41.0
20	38.72080086596273	40.0	38.0	41.0	34.0	41.0
21	38.65049467560049	40.0	38.0	41.0	34.0	41.0
22	38.593095667195115	40.0	38.0	41.0	34.0	41.0
23	38.56808893691484	40.0	38.0	41.0	34.0	41.0
24	38.515498339864195	40.0	38.0	41.0	34.0	41.0
25	38.47089480945829	40.0	38.0	41.0	34.0	41.0
26	38.27632754778499	40.0	38.0	41.0	34.0	41.0
27	38.160763494211956	40.0	38.0	41.0	33.0	41.0
28	38.07074973452784	40.0	37.0	41.0	33.0	41.0
29	37.970336289671266	40.0	37.0	41.0	33.0	41.0
30	37.890912906434146	40.0	37.0	41.0	33.0	41.0
31	37.836800050851004	40.0	37.0	41.0	33.0	41.0
32	37.73189741557237	40.0	37.0	41.0	33.0	41.0
33	37.69926396907062	40.0	37.0	41.0	33.0	41.0
34	37.61490037315665	40.0	37.0	41.0	33.0	41.0
35	37.55654977790075	40.0	37.0	41.0	33.0	41.0
36	37.512964669767584	40.0	37.0	41.0	32.0	41.0
37	37.32466264470103	40.0	36.0	41.0	32.0	41.0
38	37.21213095629805	40.0	36.0	41.0	31.0	41.0
39	37.14356809548024	40.0	36.0	41.0	31.0	41.0
40	37.042999012892224	40.0	36.0	41.0	31.0	41.0
41	36.9138556840956	39.0	36.0	41.0	31.0	41.0
42	36.817862257336	39.0	35.0	41.0	30.0	41.0
43	36.01007952947863	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	8.0
11	9.0
12	2.0
13	1.0
14	4.0
15	1.0
16	13.0
17	31.0
18	70.0
19	143.0
20	311.0
21	608.0
22	1135.0
23	1996.0
24	3406.0
25	5219.0
26	8133.0
27	12005.0
28	17120.0
29	24107.0
30	32585.0
31	42378.0
32	54847.0
33	69961.0
34	92166.0
35	122729.0
36	171515.0
37	262792.0
38	477393.0
39	738892.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.50087867548084	20.311191334390237	12.909191693338517	24.278738296790404
2	18.18517057521462	21.644815066854118	35.777188462803984	24.392825895127277
3	20.20691872812659	24.208210568035657	29.79238020101104	25.79249050282672
4	14.279458062875774	16.168797298914182	34.81732897610003	34.73441566211002
5	13.785249842960127	37.82202521611678	34.410941566211	13.981783374712093
6	33.76432054081541	35.130333747120936	16.212310430438816	14.893035281624838
7	27.921409021566806	31.217890954503307	22.253391313451587	18.6073087104783
8	26.223368654841316	34.27666312703778	20.734591397206188	18.76537682091472
9	26.158683183273013	14.256089034129998	21.082976877748198	38.50225090484879
10	16.77676595076426	27.40126117974335	33.727444213454575	22.09452865603781
11	33.97230489665281	22.57130358050911	22.324012518321318	21.132379004516764
12	20.78960209087374	26.514733705243636	30.076968233077082	22.61869597080554
13	28.987083470431635	20.897707217851693	26.562640214172472	23.552569097544197
14	22.248437079357483	22.139817833747124	26.563014118632406	29.048730968262987
15	25.034212258083816	28.36691618557626	23.772658610271904	22.82621294606802
16	24.08701878496007	27.383827884299006	25.428634725255	23.100518605485927
17	22.38294920881816	27.575968038646764	26.931216535550835	23.109866216984237
18	22.210532514731835	26.071610182166253	28.464458511561126	23.253398791540786
19	22.86079910861177	26.466920672429783	28.531995004636418	22.140285214322038
20	23.58799654512279	25.57282163261643	28.433704869731685	22.405476952529092
21	23.745644013041787	26.170134007358442	27.39971882384613	22.684503155753642
22	23.484798914181447	26.0708156351889	27.623266952828214	22.821118497801443
23	22.993441715772786	26.204813646017172	27.875278558822647	22.926466079387396
24	22.89131906015375	26.701639197152343	27.699917367114356	22.70712437557955
25	23.20254778498998	26.245849660494752	27.67439838772397	22.8772041667913
26	22.790692022374444	26.946873784810503	27.736560004187734	22.52587418862732
27	23.40553116867578	26.23795092877868	27.16850565343543	23.188012249110106
28	22.531436017468817	26.457292632586523	28.12584128503485	22.885430064909816
29	22.224460455864318	26.81535289102928	28.257502392988542	22.702684260117856
30	22.273208249828002	27.289603960396043	28.078168466393468	22.35901932338249
31	22.707918922556907	27.024365484131497	27.54605568185217	22.721659911459426
32	21.99352771379857	26.839002348120005	27.778998160390056	23.388471777691365
33	22.139256977057222	26.484727872334062	28.24048974006162	23.135525410547096
34	22.765733899673958	26.202476743142594	28.056201579372438	22.975587777811015
35	22.61271349944662	26.249869133439024	28.453101163590677	22.684316203523675
36	22.183097274984295	26.52753993299632	28.08765629206425	23.201706499955133
37	22.694785528401784	25.782067916006103	28.184637761359216	23.338508794232897
38	22.142294950794174	25.702893646615415	28.774098142442643	23.38071326014777
39	22.18052668182226	25.72588877090126	28.837989067033593	23.25559548024289
40	21.598217223535045	25.581000792677454	29.50115536478119	23.31962661900631
41	21.173882399569262	25.65568820854895	29.850148440070594	23.320280951811192
42	21.574147123926892	25.54945260387066	29.544995662708263	23.331404609494182
43	21.003802608357514	25.333382564087227	29.632816472734884	24.029998354820375
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1181.0
1	952.0
2	723.0
3	1524.0
4	2325.0
5	2325.0
6	3043.5
7	3762.0
8	3540.0
9	3318.0
10	5008.5
11	6699.0
12	6699.0
13	10895.0
14	15091.0
15	19529.0
16	23967.0
17	23617.0
18	23267.0
19	23267.0
20	27378.0
21	31489.0
22	30903.5
23	30318.0
24	33819.5
25	37321.0
26	37321.0
27	42289.0
28	47257.0
29	55272.0
30	63287.0
31	70849.5
32	78412.0
33	78412.0
34	85205.0
35	91998.0
36	99443.0
37	106888.0
38	114854.0
39	122820.0
40	122820.0
41	126657.5
42	130495.0
43	130123.5
44	129752.0
45	132888.5
46	136025.0
47	136025.0
48	136944.0
49	137863.0
50	140148.0
51	142433.0
52	146272.5
53	150112.0
54	150112.0
55	140883.5
56	131655.0
57	124807.5
58	117960.0
59	107054.0
60	96148.0
61	96148.0
62	89094.5
63	82041.0
64	72333.0
65	62625.0
66	54045.5
67	45466.0
68	45466.0
69	38617.5
70	31769.0
71	27563.0
72	23357.0
73	18393.0
74	13429.0
75	13429.0
76	10452.0
77	7475.0
78	6026.5
79	4578.0
80	3600.5
81	2623.0
82	2623.0
83	2048.0
84	1473.0
85	1232.5
86	992.0
87	861.0
88	730.0
89	730.0
90	562.5
91	395.0
92	219.0
93	43.0
94	29.0
95	15.0
96	15.0
97	10.0
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2139584.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.29716597164232
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.6259839333121	52.23301330683741
2	8.38191668585692	10.108116431351858
3	2.0348103134589595	3.680798855743333
4	0.8460820239139624	2.0406539288625294
5	0.45370426025715266	1.3678540541383368
6	0.2885991423484721	1.044102623127567
7	0.20303778905943645	0.85698222858025
8	0.14569168706098465	0.7027836668323812
9	0.11143735578905573	0.6047421063708284
>10	0.7197907235866201	8.533652271681163
>50	0.09188336382134758	3.9026231778017664
>100	0.08984921364583283	10.456108767415579
>500	0.006050038874761064	2.45243948605759
>1k	8.532106105432263E-4	0.6573418955420679
>5k	3.1025840383390045E-4	1.3587871996573246
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8920	0.41690347282462387	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8331	0.38937475696210105	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6563	0.3067418713170411	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5239	0.2448606831982292	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.347611498309952E-5	0.0	1.8695222996619905E-4	0.0
3	0.0	9.347611498309952E-5	0.0	4.673805749154976E-4	0.0
4	0.0	9.347611498309952E-5	0.0	7.010708623732464E-4	0.0
5	0.0	9.347611498309952E-5	0.0	8.412850348478956E-4	0.0
6	0.0	9.347611498309952E-5	0.0	8.412850348478956E-4	0.0
7	0.0	9.347611498309952E-5	0.0	8.880230923394454E-4	0.0
8	0.0	1.4021417247464928E-4	0.0	8.880230923394454E-4	0.0
9	0.0	1.4021417247464928E-4	0.0	0.0014021417247464928	0.0
10	0.0	1.4021417247464928E-4	0.0	0.002523855104543687	0.0
11	0.0	1.8695222996619905E-4	0.0	0.003458616254374682	0.0
12	0.0	1.8695222996619905E-4	0.0	0.004814019921629625	0.0
13	0.0	1.8695222996619905E-4	0.0	0.005234662439053573	0.0
14	0.0	1.8695222996619905E-4	0.0	0.006075947473901468	0.0
15	0.0	1.8695222996619905E-4	0.0	0.007665041428614161	0.0
16	0.0	1.8695222996619905E-4	0.0	0.01140408602793814	0.0
17	0.0	1.8695222996619905E-4	0.0	0.016264844007059315	0.0
18	0.0	1.8695222996619905E-4	0.0	0.018414794651670605	0.0
19	0.0	1.8695222996619905E-4	0.0	0.02103212587119739	0.0
20	0.0	1.8695222996619905E-4	0.0	0.024257051838114326	0.0
21	0.0	1.8695222996619905E-4	0.0	0.029959094852083396	0.0
22	0.0	1.8695222996619905E-4	0.0	0.04267184648978493	0.0
23	0.0	2.336902874577488E-4	0.0	0.060806212796506234	0.0
24	0.0	2.336902874577488E-4	0.0	0.09025118901618258	0.0
25	0.0	2.336902874577488E-4	0.0	0.09810318267476295	0.0
26	0.0	2.336902874577488E-4	0.0	0.11048876791002363	0.0
27	0.0	2.336902874577488E-4	0.0	0.13353063025335765	0.0
28	0.0	2.336902874577488E-4	0.0	0.18821415751847087	0.0
29	0.0	2.336902874577488E-4	0.0	0.2618733361251533	0.0
30	0.0	2.336902874577488E-4	0.0	0.3788119559690108	0.0
31	0.0	2.336902874577488E-4	0.0	0.6958361999341928	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4130	0.0	18.858355	1
TCCGTGA	65	0.0015805351	17.076923	8
AAGACGG	385	0.0	15.857142	5
ATCTCGC	235	0.0	15.744681	11
CGAATTA	155	7.21775E-9	15.5161295	15
GAACGTT	145	5.3520125E-8	15.310345	11
GACGGAC	390	0.0	15.179487	7
ATACCGT	220	1.8189894E-12	15.136364	6
TGTACCG	185	3.0559022E-10	15.0	5
CGGACCA	385	0.0	14.896104	9
TACGAGT	75	0.004105942	14.8	4
TACCGTC	225	1.8189894E-12	14.8	7
ACGGACC	415	0.0	14.710843	8
GCGCAAG	430	0.0	14.627907	1
CCAATAC	230	1.8189894E-12	14.478261	3
GTCTTAG	335	0.0	14.358209	1
TACGGGG	435	0.0	14.034483	2
TGCGTAC	145	8.9195237E-7	14.034483	16
ATCCGTG	80	0.0063009607	13.875	7
AGACGGA	440	0.0	13.875	6
>>END_MODULE