FastQCFastQC Report
Fri 10 Feb 2017
ERR1630522.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630522.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1737165
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29030.16711135672201546No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26280.15128096640215524No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20450.11772053892405154No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA20190.11622384747562839No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACTG451.3235932E-424.6666665
GGTATCA16950.020.0825961
GTTACCG801.6174463E-518.516
TACCGTT752.0680336E-417.26666618
TATTAGG1851.8189894E-1217.02
CGTCGTA2450.016.61224410
TATACAG2950.016.3050845
CTTATAC9200.015.8858737
CGAACTA1403.4760888E-815.85714224
GGACCGT700.0025932115.8571426
TACGTTA700.0025932115.85714219
AAGACGG3900.015.6538475
CTCGTAT1902.7284841E-1115.57894829
GTATTAG4900.015.4795911
TCTAGCG2750.015.47272828
CGAATTA1201.936789E-615.41666715
CGTATGC2055.456968E-1215.34146331
TATTAGA2550.015.2352942
GTACTAG855.366111E-415.2352941
TTACCGT855.366111E-415.23529417
Produced by FastQC (version 0.11.5)