##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630522.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1737165
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.903242351762785	33.0	31.0	34.0	30.0	34.0
2	32.07243641220034	33.0	31.0	34.0	30.0	34.0
3	32.18775821525301	34.0	31.0	34.0	30.0	34.0
4	35.78868904220382	37.0	35.0	37.0	35.0	37.0
5	35.759998618438665	37.0	35.0	37.0	35.0	37.0
6	35.78966937510254	37.0	35.0	37.0	35.0	37.0
7	35.76821545449051	37.0	35.0	37.0	35.0	37.0
8	35.75338669613998	37.0	35.0	37.0	33.0	37.0
9	37.47806685029919	39.0	37.0	39.0	35.0	39.0
10	37.36417150932698	39.0	37.0	39.0	34.0	39.0
11	37.47846174658135	39.0	37.0	39.0	35.0	39.0
12	37.393901557998234	39.0	37.0	39.0	34.0	39.0
13	37.4555111345209	39.0	37.0	39.0	35.0	39.0
14	38.70481848298809	40.0	38.0	41.0	35.0	41.0
15	38.67912547167368	40.0	38.0	41.0	35.0	41.0
16	38.66395823079558	40.0	38.0	41.0	34.0	41.0
17	38.63895657580023	40.0	38.0	41.0	34.0	41.0
18	38.62623987934364	40.0	38.0	41.0	34.0	41.0
19	38.63775346613592	40.0	38.0	41.0	34.0	41.0
20	38.62281533417954	40.0	38.0	41.0	34.0	41.0
21	38.54531837793186	40.0	38.0	41.0	34.0	41.0
22	38.491554918502274	40.0	38.0	41.0	34.0	41.0
23	38.46726937279993	40.0	38.0	41.0	34.0	41.0
24	38.4111538051941	40.0	38.0	41.0	34.0	41.0
25	38.37241367400333	40.0	38.0	41.0	34.0	41.0
26	38.186599430681596	40.0	38.0	41.0	33.0	41.0
27	38.073521513500445	40.0	38.0	41.0	33.0	41.0
28	37.97259615522993	40.0	37.0	41.0	33.0	41.0
29	37.87445349175237	40.0	37.0	41.0	33.0	41.0
30	37.81029666151459	40.0	37.0	41.0	33.0	41.0
31	37.77925067566984	40.0	37.0	41.0	33.0	41.0
32	37.69799529693495	40.0	37.0	41.0	32.0	41.0
33	37.66693952503073	40.0	37.0	41.0	32.0	41.0
34	37.600301640891914	40.0	37.0	41.0	32.0	41.0
35	37.55125909168098	40.0	37.0	41.0	32.0	41.0
36	37.53357107701341	40.0	37.0	41.0	32.0	41.0
37	37.35592358814505	40.0	36.0	41.0	31.0	41.0
38	37.25124786649512	40.0	36.0	41.0	31.0	41.0
39	37.20779200594071	39.0	36.0	41.0	31.0	41.0
40	37.10565893280143	39.0	36.0	41.0	31.0	41.0
41	37.00825079943471	39.0	36.0	41.0	31.0	41.0
42	36.93734158816232	39.0	35.0	41.0	31.0	41.0
43	36.06468067224472	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	1.0
10	4.0
11	3.0
12	0.0
13	0.0
14	2.0
15	7.0
16	11.0
17	25.0
18	54.0
19	122.0
20	244.0
21	493.0
22	872.0
23	1622.0
24	2762.0
25	4268.0
26	6611.0
27	9891.0
28	13986.0
29	19596.0
30	26236.0
31	34557.0
32	44669.0
33	57750.0
34	76895.0
35	102922.0
36	143146.0
37	217202.0
38	401965.0
39	571246.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.93566529374009	18.929289963820363	11.706026773507409	25.42901796893214
2	19.868176022427345	20.30215897741435	33.336902366787264	26.492762633371036
3	20.973252396865007	22.36258501639165	28.68938759415484	27.974774992588497
4	14.690256826496043	14.706893127595825	34.217935544407126	36.38491450150101
5	15.08595901943684	36.219184706115996	33.9427745781201	14.752081696327062
6	36.435341490301724	34.05991946648707	14.85938296016786	14.645356083043348
7	30.13001067831783	29.595864526397897	20.731594293000377	19.542530502283896
8	28.309803616812452	31.763706959327408	19.508854944694374	20.417634479165766
9	26.949598915474347	13.109117441348403	19.426018829529724	40.51526481364752
10	17.49275399861268	25.77665334035627	32.62113846410675	24.1094541969243
11	37.18057870150504	20.804529218583152	20.780524590352673	21.23436748955914
12	22.856781019649834	24.88174698431064	27.719186145242393	24.542285850797132
13	31.032573186772698	19.064395149568405	24.208350962631645	25.694680701027252
14	23.415507450357335	20.637187601638303	24.238745312045776	31.70855963595859
15	26.6702357001206	27.327858896535446	21.387893493133927	24.614011910210028
16	26.474572075767128	25.64022415832693	22.974789383852425	24.91041438205352
17	24.64239148267436	25.48341694657675	24.480345850854697	25.3938457198942
18	24.661963601615273	24.040318565018293	26.08382047761727	25.213897355749165
19	25.243025273937707	24.64187339717298	25.547774678858946	24.567326650030367
20	25.956429009334176	23.95748244985364	24.96792187270639	25.118166668105797
21	26.35869361862575	24.378110311916252	24.748080924955314	24.51511514450268
22	26.066550960904696	24.260389772992202	24.99929482806757	24.673764438035533
23	25.301108415147667	24.125054327021324	25.050988248093876	25.52284900973713
24	25.012074270434876	24.894353731510822	25.06883341536354	25.024738582690766
25	25.456015980059465	24.618501984555298	24.775712151695434	25.149769883689803
26	25.18367570150216	25.259891835260323	24.78682220744719	24.769610255790326
27	25.580183805222877	24.10127995901368	24.889345571664176	25.429190664099266
28	24.718780311599648	24.556331724390027	25.366732578655455	25.358155385354873
29	24.364524958769028	25.103832969234357	25.75057637012028	24.781065701876333
30	24.667259586740464	25.342727950424976	25.244867355720384	24.745145107114176
31	24.9183583597413	25.639763637881263	24.65482553470741	24.787052467670026
32	24.43222146428232	24.807833452780827	24.89279947500669	25.867145607930166
33	24.071403695100926	24.788434029007032	25.620479344218882	25.519682931673156
34	25.409618545158345	24.064841278750148	25.42291607302703	25.102624103064475
35	24.963489363416834	24.334303304522024	25.80670229943615	24.895505032624996
36	24.004282840144718	25.356313303572197	25.502758805294835	25.13664505098825
37	25.08092207706234	24.04152743118817	25.847746184156367	25.029804307593118
38	24.397509735690047	24.062193286187554	26.178112038867923	25.362184939254472
39	24.458298434518312	23.884662654382282	26.246441759994017	25.410597151105392
40	23.770453583856455	23.83147254290755	27.057245569649403	25.340828303586594
41	23.158537041674222	23.965426427541423	27.523407390777503	25.35262914000685
42	23.762394476057256	23.845748676723282	27.625642929715944	24.766213917503517
43	23.29675074043053	23.21218767359462	27.539295346153068	25.951766239821776
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	77.0
1	76.5
2	76.0
3	248.0
4	420.0
5	420.0
6	596.0
7	772.0
8	774.5
9	777.0
10	1130.5
11	1484.0
12	1484.0
13	2544.5
14	3605.0
15	5182.0
16	6759.0
17	6910.5
18	7062.0
19	7062.0
20	9303.0
21	11544.0
22	12285.0
23	13026.0
24	15850.5
25	18675.0
26	18675.0
27	21829.0
28	24983.0
29	29566.0
30	34149.0
31	39620.5
32	45092.0
33	45092.0
34	51835.0
35	58578.0
36	65819.5
37	73061.0
38	79292.5
39	85524.0
40	85524.0
41	90353.5
42	95183.0
43	97725.0
44	100267.0
45	103083.5
46	105900.0
47	105900.0
48	110848.5
49	115797.0
50	116832.5
51	117868.0
52	120959.0
53	124050.0
54	124050.0
55	122754.5
56	121459.0
57	119299.0
58	117139.0
59	114143.5
60	111148.0
61	111148.0
62	103605.5
63	96063.0
64	87247.5
65	78432.0
66	68709.0
67	58986.0
68	58986.0
69	50812.0
70	42638.0
71	36266.0
72	29894.0
73	22849.5
74	15805.0
75	15805.0
76	11895.5
77	7986.0
78	6504.0
79	5022.0
80	4118.0
81	3214.0
82	3214.0
83	2487.0
84	1760.0
85	1506.0
86	1252.0
87	1106.0
88	960.0
89	960.0
90	775.5
91	591.0
92	326.0
93	61.0
94	41.0
95	21.0
96	21.0
97	13.0
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1737165.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.715442096529486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.95186571930567	54.279882760815454
2	7.888400866424576	9.736722938120778
3	1.7259067226618634	3.1954528921934755
4	0.6863236052991493	1.6942705888928398
5	0.35508108306372504	1.095699301069615
6	0.22780515169864166	0.8435457389369181
7	0.15247068346936277	0.6586856945950212
8	0.11378618643768981	0.5617891840384124
9	0.09883944439816955	0.5489928006847541
>10	0.6149809706647025	7.678393333649612
>50	0.08562868844151175	3.72256157804871
>100	0.08984287704009235	11.239504065037538
>500	0.007011341052761164	2.917894366049665
>1k	0.0020566600421432746	1.8266047578671754
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2903	0.16711135672201546	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2628	0.15128096640215524	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2045	0.11772053892405154	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2019	0.11622384747562839	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.332156127944093E-4	0.0
3	0.0	0.0	0.0	9.210408913373226E-4	0.0
4	0.0	0.0	0.0	0.0016118215598403146	0.0
5	0.0	0.0	0.0	0.0017845167269660625	0.0
6	0.0	0.0	0.0	0.0018420817826746451	0.0
7	0.0	0.0	0.0	0.0020723420055089757	0.0
8	0.0	0.0	0.0	0.002245037172634724	0.0
9	0.0	0.0	0.0	0.0033387732310977943	0.0
10	0.0	0.0	0.0	0.0043749442338522826	0.0
11	0.0	0.0	0.0	0.004720334568103778	0.0
12	0.0	0.0	0.0	0.005123289958063857	0.0
13	0.0	0.0	0.0	0.005526245348023935	0.0
14	0.0	0.0	0.0	0.005756505570858266	0.0
15	0.0	0.0	0.0	0.006447286239361258	0.0
16	0.0	0.0	0.0	0.007943977687784407	0.0
17	0.0	0.0	0.0	0.009440669136207556	0.0
18	0.0	0.0	0.0	0.010131449804710549	0.0
19	0.0	0.0	0.0	0.011110055751756455	0.0
20	0.0	0.0	0.0	0.012146226754510941	0.0
21	0.0	0.0	0.0	0.013527788091516925	0.0
22	0.0	0.0	0.0	0.01703925648974047	0.0
23	0.0	0.0	0.0	0.021817156113552828	0.0
24	0.0	0.0	0.0	0.03045191446984023	0.0
25	0.0	0.0	0.0	0.032812081753892115	0.0
26	0.0	0.0	0.0	0.037244591043452985	0.0
27	0.0	0.0	0.0	0.045994479511157545	0.0
28	0.0	1.1513011141716532E-4	0.0	0.07097771368868243	0.0
29	0.0	2.878252785429133E-4	0.0	0.11052490696047872	0.0
30	0.0	2.878252785429133E-4	0.0	0.1630818028224147	0.0
31	0.0	2.878252785429133E-4	0.0	0.3656532338609171	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGACTG	45	1.3235932E-4	24.666666	5
GGTATCA	1695	0.0	20.082596	1
GTTACCG	80	1.6174463E-5	18.5	16
TACCGTT	75	2.0680336E-4	17.266666	18
TATTAGG	185	1.8189894E-12	17.0	2
CGTCGTA	245	0.0	16.612244	10
TATACAG	295	0.0	16.305084	5
CTTATAC	920	0.0	15.88587	37
CGAACTA	140	3.4760888E-8	15.857142	24
GGACCGT	70	0.00259321	15.857142	6
TACGTTA	70	0.00259321	15.857142	19
AAGACGG	390	0.0	15.653847	5
CTCGTAT	190	2.7284841E-11	15.578948	29
GTATTAG	490	0.0	15.479591	1
TCTAGCG	275	0.0	15.472728	28
CGAATTA	120	1.936789E-6	15.416667	15
CGTATGC	205	5.456968E-12	15.341463	31
TATTAGA	255	0.0	15.235294	2
GTACTAG	85	5.366111E-4	15.235294	1
TTACCGT	85	5.366111E-4	15.235294	17
>>END_MODULE