FastQCFastQC Report
Fri 10 Feb 2017
ERR1630521.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630521.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2216160
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT97750.4410782614973648No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT86120.3886001010757346No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT70340.31739585589488123No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54970.24804165764204752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG2850.019.47368429
TACACCG1254.129106E-1019.245
TTAGCGC500.007036892718.53
GACCGTT701.21984514E-418.57
TCTAGCG3050.018.1967228
GGTATCA47150.017.3032881
CGCAATA3150.017.03174636
TGCGAAC700.002593511215.85714329
ACCGTCG2800.015.8571438
CCGTCGT2700.015.7592599
CGTCGTA2600.015.65384610
TACCGTC2950.015.0508477
GTATAGA2006.184564E-1114.81
TAGTCGT1001.0940913E-414.86
ATACCGT3200.014.4531266
AAGACGG4050.014.1604945
CGCAAGA4150.013.8192772
CGGTCCA4450.013.71910210
TTAGTCG950.001245711813.6315795
TAGACTA1102.4579733E-413.4545455
Produced by FastQC (version 0.11.5)