##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630521.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2216160
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.991451880730633	33.0	31.0	34.0	30.0	34.0
2	32.15039166847159	33.0	31.0	34.0	30.0	34.0
3	32.249846130243306	34.0	31.0	34.0	30.0	34.0
4	35.83684210526316	37.0	35.0	37.0	35.0	37.0
5	35.81166747888239	37.0	35.0	37.0	35.0	37.0
6	35.8480109739369	37.0	35.0	37.0	35.0	37.0
7	35.8256691755108	37.0	35.0	37.0	35.0	37.0
8	35.81827485380117	37.0	35.0	37.0	35.0	37.0
9	37.54034771857628	39.0	37.0	39.0	35.0	39.0
10	37.44202810266407	39.0	37.0	39.0	34.0	39.0
11	37.5556056421919	39.0	37.0	39.0	35.0	39.0
12	37.482535105768534	39.0	37.0	39.0	35.0	39.0
13	37.53341816475345	39.0	37.0	39.0	35.0	39.0
14	38.80259593170168	40.0	38.0	41.0	35.0	41.0
15	38.775383546314345	40.0	38.0	41.0	35.0	41.0
16	38.753084614829255	40.0	38.0	41.0	35.0	41.0
17	38.74503059345896	40.0	38.0	41.0	35.0	41.0
18	38.7373095805357	40.0	38.0	41.0	35.0	41.0
19	38.76806277525088	40.0	38.0	41.0	35.0	41.0
20	38.74380459894592	40.0	38.0	41.0	34.0	41.0
21	38.67656080788391	40.0	38.0	41.0	34.0	41.0
22	38.61669374052415	40.0	38.0	41.0	34.0	41.0
23	38.58893446321565	40.0	38.0	41.0	34.0	41.0
24	38.53825761677857	40.0	38.0	41.0	34.0	41.0
25	38.49477068442712	40.0	38.0	41.0	34.0	41.0
26	38.297946899140854	40.0	38.0	41.0	34.0	41.0
27	38.18372048949534	40.0	38.0	41.0	34.0	41.0
28	38.09034185257382	40.0	38.0	41.0	33.0	41.0
29	37.98568424662479	40.0	37.0	41.0	33.0	41.0
30	37.902632481409285	40.0	37.0	41.0	33.0	41.0
31	37.845266587250016	40.0	37.0	41.0	33.0	41.0
32	37.7372870189878	40.0	37.0	41.0	33.0	41.0
33	37.689633871200634	40.0	37.0	41.0	33.0	41.0
34	37.6011456754025	40.0	37.0	41.0	33.0	41.0
35	37.54820094217024	40.0	37.0	41.0	32.0	41.0
36	37.49180158472313	40.0	37.0	41.0	32.0	41.0
37	37.30055636777128	40.0	36.0	41.0	32.0	41.0
38	37.18233024691358	40.0	36.0	41.0	31.0	41.0
39	37.10162082160133	40.0	36.0	41.0	31.0	41.0
40	36.98951429499675	39.0	36.0	41.0	31.0	41.0
41	36.84778580968883	39.0	35.0	41.0	31.0	41.0
42	36.744823929680166	39.0	35.0	41.0	30.0	41.0
43	35.89330914735398	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	2.0
10	7.0
11	5.0
12	4.0
13	4.0
14	2.0
15	12.0
16	7.0
17	27.0
18	64.0
19	149.0
20	320.0
21	598.0
22	1168.0
23	2023.0
24	3325.0
25	5330.0
26	8304.0
27	12245.0
28	17848.0
29	24953.0
30	33436.0
31	43591.0
32	55759.0
33	72711.0
34	95514.0
35	126792.0
36	177872.0
37	274431.0
38	491221.0
39	768433.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.78402281423724	19.29801999855606	12.326952927586456	24.591004259620245
2	18.762092989675835	21.039726373547037	34.97414446610353	25.224036170673596
3	20.656360551584722	23.712547830481554	28.94046458739441	26.69062703053931
4	14.62768933651	15.637363728250667	33.915466392318244	35.81948054292109
5	14.523725723774456	37.17042090823767	33.68791062017183	14.617942747816043
6	35.02165908598657	34.0127969099704	15.838387120063532	15.127156883979495
7	29.050068587105628	30.495000360984765	21.594153851707457	18.86077720020215
8	27.292884990253413	33.50074001877121	20.016379683777345	19.189995307198036
9	26.788363656053715	13.872509205111545	20.178191105335355	39.16093603349939
10	17.50067684643708	26.72063388924987	32.607347844920945	23.171341419392103
11	35.37037037037037	21.79648581329868	21.45864919500397	21.37449462132698
12	21.622265540394196	25.82679048444156	29.176999855606095	23.373944119558153
13	29.809625658797202	20.07851418670132	25.749269005847953	24.362591148653525
14	22.862157966933797	21.134529997834093	25.80625947585012	30.197052559381994
15	25.87028914879792	27.48560573243809	22.959082376723703	23.685022742040285
16	25.051711067792937	26.341374269005847	24.712295141145045	23.894619522056168
17	23.500469280196377	26.600155223449573	26.005703559309794	23.893671937044257
18	23.279862464803987	25.130044762111037	27.506858710562415	24.083234062522564
19	23.89597321493033	25.558984910836763	27.497518229730705	23.047523644502203
20	24.316836329506895	24.72186123745578	27.539798570500324	23.421503862537
21	24.640278680239692	25.236309652732654	26.78552090101798	23.337890766009675
22	24.47287199480182	25.089208360407188	26.835833152840948	23.60208649195004
23	23.646532741318318	25.427586455851564	27.05228864341925	23.873592159410872
24	23.818948090390585	25.792181069958847	26.845128510576856	23.543742329073712
25	24.16887769836113	25.171964118114214	26.861959425312254	23.797198758212403
26	23.86429680167497	25.938470146559816	26.88989062161577	23.307342430149447
27	24.198297956826224	25.308280990542197	26.54970760233918	23.943713450292396
28	23.33540899574038	25.64413219262147	27.337150747238464	23.683308064399682
29	23.20825211176088	25.854315572882825	27.363051404230742	23.57438091112555
30	23.072702331961594	26.356670998483867	27.305293841599887	23.26533282795466
31	23.59613024330373	26.068875893437298	26.686881813587465	23.648112049671504
32	22.96503862537001	25.937657930835318	27.031712511732003	24.065590932062666
33	22.98083170890188	25.818939065771424	27.35371092339903	23.84651830192766
34	23.746751137102017	25.470047289004405	27.107970543643056	23.675231030250522
35	23.53309327846365	25.46539961013645	27.455824489206552	23.545682622193343
36	22.964903256082593	25.930212620027437	27.080986932351454	24.023897191538516
37	23.472673453180278	25.104324597501986	27.306737780665657	24.116264168652084
38	22.945410078694678	25.045709696050828	27.847312468413833	24.16156775684066
39	23.044861381849685	24.95907335210454	27.86757273843044	24.128492527615332
40	22.66122482131254	24.982762977402352	28.40557540971771	23.950436791567395
41	22.08265648689625	25.131894809039057	28.781315428488917	24.00413327557577
42	22.534654537578515	25.094126777849972	28.598205905710778	23.773012778860732
43	22.022778138762543	24.752048588549563	28.573478449209443	24.651694823478447
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	654.0
1	601.0
2	548.0
3	1413.0
4	2278.0
5	2278.0
6	2911.5
7	3545.0
8	3393.0
9	3241.0
10	4845.0
11	6449.0
12	6449.0
13	10825.5
14	15202.0
15	19572.5
16	23943.0
17	22863.5
18	21784.0
19	21784.0
20	24940.0
21	28096.0
22	25876.0
23	23656.0
24	26038.0
25	28420.0
26	28420.0
27	32885.5
28	37351.0
29	43714.5
30	50078.0
31	56869.0
32	63660.0
33	63660.0
34	71093.0
35	78526.0
36	87244.5
37	95963.0
38	106519.0
39	117075.0
40	117075.0
41	122807.5
42	128540.0
43	131590.5
44	134641.0
45	139016.5
46	143392.0
47	143392.0
48	147871.0
49	152350.0
50	154502.0
51	156654.0
52	159577.0
53	162500.0
54	162500.0
55	154366.0
56	146232.0
57	139315.5
58	132399.0
59	123435.0
60	114471.0
61	114471.0
62	106387.0
63	98303.0
64	87273.5
65	76244.0
66	66749.0
67	57254.0
68	57254.0
69	48667.5
70	40081.0
71	34708.0
72	29335.0
73	23433.0
74	17531.0
75	17531.0
76	13976.0
77	10421.0
78	8422.5
79	6424.0
80	5146.0
81	3868.0
82	3868.0
83	2939.5
84	2011.0
85	1716.0
86	1421.0
87	1218.0
88	1015.0
89	1015.0
90	765.5
91	516.0
92	290.5
93	65.0
94	42.5
95	20.0
96	20.0
97	11.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2216160.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.508052373030495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.00748127767966	49.1511411432086
2	9.31921565083741	10.904983147495214
3	2.657912110870219	4.665277829571204
4	1.2198048394162613	2.854736217177707
5	0.6727708729696861	1.9681256735379917
6	0.43352360398271816	1.5218773036059483
7	0.2905756611022291	1.1900711198668024
8	0.20787642459398115	0.9729955789810377
9	0.15335412498983778	0.8075206058873123
>10	0.8537703856559142	9.239863309313264
>50	0.09942327969536442	4.113268571670309
>100	0.0788810355717182	8.900917297705394
>500	0.004251289927593353	1.6704117541552512
>1k	8.502579855186596E-4	0.6405596731063121
>5k	3.091847220067853E-4	1.3982507747176616
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9775	0.4410782614973648	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8612	0.3886001010757346	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7034	0.31739585589488123	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5497	0.24804165764204752	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.024619161071403E-5	0.0
2	0.0	0.0	0.0	4.061078622482131E-4	0.0
3	0.0	0.0	0.0	6.317233412749982E-4	0.0
4	0.0	0.0	0.0	9.475850119124973E-4	0.0
5	0.0	4.512309580535702E-5	0.0	0.0011280773951339253	0.0
6	0.0	4.512309580535702E-5	0.0	0.0011280773951339253	0.0
7	0.0	4.512309580535702E-5	0.0	0.0012634466825499964	0.0
8	0.0	4.512309580535702E-5	0.0	0.0014439390657714245	0.0
9	0.0	4.512309580535702E-5	0.0	0.002526893365099993	0.0
10	0.0	9.024619161071403E-5	0.0	0.004467186484730344	0.0
11	0.0	9.024619161071403E-5	0.0	0.005459894592448199	0.0
12	0.0	9.024619161071403E-5	0.0	0.006723341274998195	0.0
13	0.0	9.024619161071403E-5	0.0	0.0074453108078839075	0.0
14	0.0	1.3536928741607104E-4	0.0	0.00830264962818569	0.0
15	0.0	1.3536928741607104E-4	0.0	0.0099722041729839	0.0
16	0.0	1.3536928741607104E-4	0.0	0.013491805645801748	0.0
17	0.0	1.3536928741607104E-4	0.0	0.019132192621471374	0.0
18	0.0	1.3536928741607104E-4	0.0	0.02129810122012851	0.0
19	0.0	1.3536928741607104E-4	0.0	0.024366471734892786	0.0
20	0.0	1.3536928741607104E-4	0.0	0.026712872716771353	0.0
21	0.0	1.3536928741607104E-4	0.0	0.032578875171467764	0.0
22	0.0	1.3536928741607104E-4	0.0	0.044626741751498084	0.0
23	0.0	1.3536928741607104E-4	0.0	0.06321745722330517	0.0
24	0.0	1.3536928741607104E-4	0.0	0.09051693018554617	0.0
25	0.0	1.3536928741607104E-4	0.0	0.09773662551440329	0.0
26	0.0	1.3536928741607104E-4	0.0	0.11014547686087647	0.0
27	0.0	1.3536928741607104E-4	0.0	0.1302703775900657	0.0
28	0.0	1.3536928741607104E-4	0.0	0.1767922893653888	0.0
29	0.0	1.3536928741607104E-4	0.0	0.2413183163670493	0.0
30	0.0	1.3536928741607104E-4	0.0	0.33900981878564723	0.0
31	0.0	1.3536928741607104E-4	0.0	0.6210742906649339	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	285	0.0	19.473684	29
TACACCG	125	4.129106E-10	19.24	5
TTAGCGC	50	0.0070368927	18.5	3
GACCGTT	70	1.21984514E-4	18.5	7
TCTAGCG	305	0.0	18.19672	28
GGTATCA	4715	0.0	17.303288	1
CGCAATA	315	0.0	17.031746	36
TGCGAAC	70	0.0025935112	15.857143	29
ACCGTCG	280	0.0	15.857143	8
CCGTCGT	270	0.0	15.759259	9
CGTCGTA	260	0.0	15.653846	10
TACCGTC	295	0.0	15.050847	7
GTATAGA	200	6.184564E-11	14.8	1
TAGTCGT	100	1.0940913E-4	14.8	6
ATACCGT	320	0.0	14.453126	6
AAGACGG	405	0.0	14.160494	5
CGCAAGA	415	0.0	13.819277	2
CGGTCCA	445	0.0	13.719102	10
TTAGTCG	95	0.0012457118	13.631579	5
TAGACTA	110	2.4579733E-4	13.454545	5
>>END_MODULE