FastQCFastQC Report
Fri 10 Feb 2017
ERR1630520.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630520.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296171
Sequences flagged as poor quality0
Sequence length43
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT119844.046311083799562No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT115483.899098831418336No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT79922.69844110328155No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60482.0420635376184704No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT17730.5986406501649385No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16250.5486695186226875No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15940.5382025924212701No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15090.509502956062545No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT12350.41698883415324256Illumina PCR Primer Index 10 (95% over 21bp)
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA12280.4146253346884064No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA11080.37410820100550024No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10430.3521614202605927No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9650.3258252833667037No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT8490.2866587208065611No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA8350.28193172187688864No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA8250.27855529406997986No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7430.2508685860533273No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA7340.24782980102710933No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG6680.22554537750151096No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC6620.22351952081736565No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6350.21440316573871177No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG6110.20629973900213053No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA6070.20494916787936698No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC5980.20191038285314902No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA5590.18874231440620454No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5460.18435295825722303No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA5410.1826647443537686No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA5130.17321074649442383No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA4950.1671331764419879No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4830.16308146307369728No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA4700.1586921069247158No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC4430.1495757518460619No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA4290.14484875291638952No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT4270.14417346735500775No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3840.12965482778529971No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC3500.11817497324180964No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA3480.11749968768042787No Hit
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA3320.11209740318937371No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA3270.11040918928591928No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT3260.1100715465052284No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3260.1100715465052284No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA3070.1036563336721016No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGAG200.001839730437.02
TTCTGAC200.001839730437.026
ACTGTGC303.5930853E-430.8333348
AGCGTGG250.00548983829.618
TGTGCGC250.00548983829.610
GATACAC556.235405E-726.909093
CCGTCTT1650.026.9090937
TAGTACT358.8537915E-426.428574
GCCGTCT1700.026.11764736
ATGGGTC451.3199744E-424.6666665
AGTGGCT705.0808485E-621.14285727
ATACACT705.0808485E-621.1428574
CGTCTTC450.003819205320.55555537
GTGCTGG450.003819205320.5555551
TACACTG450.003819205320.5555555
GTGTATA555.1307137E-420.1818189
ACAGAGG555.1307137E-420.1818183
GAAACGC759.227742E-619.7333349
ATGCCCC759.227742E-619.73333437
TGCCGTC2300.019.30434835
Produced by FastQC (version 0.11.5)