##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630520.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	296171
Sequences flagged as poor quality	0
Sequence length	43
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.846581873309677	33.0	31.0	34.0	30.0	34.0
2	31.846021386293728	33.0	31.0	34.0	30.0	34.0
3	31.823173099324375	33.0	31.0	34.0	30.0	34.0
4	35.532344490176285	37.0	35.0	37.0	33.0	37.0
5	35.58687042283005	37.0	35.0	37.0	33.0	37.0
6	35.71345945416668	37.0	35.0	37.0	35.0	37.0
7	35.63323215304672	37.0	35.0	37.0	33.0	37.0
8	35.74856755050292	37.0	35.0	37.0	35.0	37.0
9	37.271144035033814	39.0	37.0	39.0	34.0	39.0
10	37.232642628751634	39.0	37.0	39.0	34.0	39.0
11	37.42721941040818	39.0	37.0	39.0	34.0	39.0
12	37.4136934406137	39.0	37.0	39.0	34.0	39.0
13	37.4869011483231	39.0	37.0	39.0	35.0	39.0
14	38.48148198169301	40.0	38.0	41.0	34.0	41.0
15	38.41809967890172	40.0	38.0	41.0	34.0	41.0
16	38.27164712277705	40.0	38.0	41.0	34.0	41.0
17	38.48102954036688	40.0	38.0	41.0	34.0	41.0
18	38.62988948951788	40.0	38.0	41.0	34.0	41.0
19	38.75292314237383	40.0	38.0	41.0	35.0	41.0
20	38.79508797282651	40.0	38.0	41.0	35.0	41.0
21	38.71372956839123	40.0	38.0	41.0	35.0	41.0
22	38.57595105530251	40.0	38.0	41.0	34.0	41.0
23	38.45941702597486	40.0	38.0	41.0	34.0	41.0
24	38.343649445759375	40.0	37.0	41.0	34.0	41.0
25	38.22391118644297	40.0	37.0	41.0	34.0	41.0
26	37.7779289667118	40.0	36.0	41.0	33.0	41.0
27	37.46022736864852	39.0	35.0	41.0	33.0	41.0
28	37.23656266143546	39.0	35.0	41.0	33.0	41.0
29	36.91441093152267	39.0	35.0	41.0	32.0	41.0
30	36.54415185821704	38.0	35.0	40.0	32.0	41.0
31	36.0854742699319	38.0	35.0	40.0	31.0	41.0
32	35.638141479077966	37.0	35.0	40.0	30.0	41.0
33	35.263219558971	35.0	35.0	40.0	30.0	41.0
34	34.874305722032204	35.0	35.0	40.0	27.0	41.0
35	34.49302598836483	35.0	34.0	40.0	25.0	41.0
36	34.0265859925516	35.0	33.0	40.0	21.0	41.0
37	33.38305573469381	35.0	33.0	40.0	18.0	41.0
38	32.83519993517259	35.0	33.0	40.0	15.0	41.0
39	32.23924692154195	35.0	33.0	40.0	10.0	41.0
40	31.595135918101366	35.0	31.0	39.0	9.0	41.0
41	30.880606136319898	35.0	30.0	39.0	7.0	41.0
42	30.204307646596053	35.0	25.0	39.0	7.0	41.0
43	29.225737833886505	35.0	22.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	3.0
12	3.0
13	4.0
14	4.0
15	3.0
16	4.0
17	8.0
18	23.0
19	49.0
20	128.0
21	233.0
22	455.0
23	768.0
24	1204.0
25	1987.0
26	2837.0
27	4277.0
28	5945.0
29	7637.0
30	9214.0
31	10646.0
32	12867.0
33	15776.0
34	18987.0
35	22768.0
36	29810.0
37	53997.0
38	50125.0
39	46404.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.321027379453085	19.454639380628084	14.485550577200335	14.738782662718497
2	16.245007107380534	26.94558211303605	32.83339692272369	23.97601385685972
3	24.856248586120856	30.79268395622799	29.92224086760689	14.428826590044263
4	13.420287604120592	18.35088513054958	27.7957666348157	40.43306063051413
5	9.948644533056916	47.12277704434263	28.478142694591973	14.450435728008483
6	28.927882878472232	28.736101779039814	20.579665125890113	21.756350216597845
7	22.654817656016288	38.27045862018901	22.01397165826499	17.060752065529712
8	35.65912935432571	28.579097886018552	20.1170945163436	15.644678243312141
9	29.44211283346445	10.5604532516688	24.341005702786568	35.65642821208018
10	20.79406829162882	33.9888105182479	24.88089650911129	20.336224681011984
11	25.226980359319445	27.176192132247923	19.199381438425775	28.397446070006854
12	19.12847645448069	35.96908542699994	28.98224336616347	15.9201947523559
13	30.536750728464302	20.43515401575441	32.3255821805646	16.702513075216682
14	22.80945804957271	24.962268419257793	34.3592046486658	17.86906888250369
15	28.0030793021599	24.83835351874424	31.416310172164053	15.742257006931807
16	18.36439084177722	25.878968568833542	36.257770004490645	19.49887058489859
17	15.12909771719716	33.14369063817862	34.29133844974694	17.43587319487728
18	14.381894243528231	24.317708350918895	39.791876989982136	21.508520415570732
19	14.914019265897066	30.116047823723456	42.35694919489079	12.612983715488687
20	16.313886234641476	24.743138254589407	44.04651366946798	14.89646184130114
21	19.64540755171843	25.36676447052547	41.010092142714846	13.977735835041244
22	18.142897177643995	27.080639225312403	39.40460072052969	15.371862876513905
23	16.420918996120484	28.00172873103714	39.80605798677115	15.771294286071221
24	14.246499488471187	30.306478352033118	40.93716130208562	14.509860857410079
25	13.508412369880912	30.244689723166683	40.816960472159664	15.429937434792738
26	15.420145794152704	30.11402196703931	40.24735710113414	14.218475137673844
27	13.364914188087287	31.844441218080092	39.942803312950964	14.847841280881651
28	13.12181138598985	31.3811953229722	41.664781494474475	13.83221179656347
29	12.566051368972655	32.93367682858889	41.02832485287215	13.471946949566298
30	12.024472348744474	33.36856073011875	41.87614587518697	12.730821045949806
31	11.94985329421179	34.013796084019035	40.21494339418782	13.821407227581364
32	11.26308787828653	34.68435464647113	40.79231254916923	13.260244926073113
33	12.088286834295053	34.30113009038697	40.862204604772245	12.748378470545733
34	11.997123283508513	35.21614202605927	38.16275057314862	14.623984117283594
35	11.653402932765193	35.09053891164226	39.3377474499529	13.918310705639648
36	11.572368665399381	35.7742655425413	37.24233635298527	15.411029439074047
37	11.661506359501775	35.73712483666531	37.71773738819804	14.883631415634888
38	12.009616066394077	34.056001431605395	38.052679026643396	15.881703475357142
39	12.740950329370532	34.2741186679317	37.79910929834453	15.185821704353227
40	11.951879150895934	34.21503118131079	38.093533803106986	15.739555864686281
41	11.493697897498405	35.25497094583872	36.458667459001724	16.792663697661148
42	11.742202984086896	34.47467847966209	37.05933396585081	16.72378457040021
43	10.945028378875717	35.49807374793616	36.325636203409516	17.231261669778608
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	29.0
1	95.5
2	162.0
3	755.0
4	1348.0
5	1348.0
6	2141.0
7	2934.0
8	3138.0
9	3342.0
10	4895.5
11	6449.0
12	6449.0
13	11480.0
14	16511.0
15	22603.5
16	28696.0
17	25419.0
18	22142.0
19	22142.0
20	23793.5
21	25445.0
22	18381.5
23	11318.0
24	9895.5
25	8473.0
26	8473.0
27	8650.5
28	8828.0
29	8674.0
30	8520.0
31	8242.0
32	7964.0
33	7964.0
34	7311.5
35	6659.0
36	6262.5
37	5866.0
38	6473.5
39	7081.0
40	7081.0
41	8104.5
42	9128.0
43	10086.5
44	11045.0
45	13401.0
46	15757.0
47	15757.0
48	17660.0
49	19563.0
50	18643.0
51	17723.0
52	13589.0
53	9455.0
54	9455.0
55	9472.0
56	9489.0
57	8682.5
58	7876.0
59	6682.0
60	5488.0
61	5488.0
62	5007.0
63	4526.0
64	4167.0
65	3808.0
66	3436.5
67	3065.0
68	3065.0
69	2791.0
70	2517.0
71	2254.0
72	1991.0
73	1681.0
74	1371.0
75	1371.0
76	1121.0
77	871.0
78	644.0
79	417.0
80	303.5
81	190.0
82	190.0
83	135.5
84	81.0
85	55.5
86	30.0
87	18.5
88	7.0
89	7.0
90	6.5
91	6.0
92	3.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	296171.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.82931993657638
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.22814127375705	28.810892430148943
2	11.412436881393088	8.4062457832162
3	3.622964161583283	4.0029391867710284
4	1.7257366564018235	2.5423082977960134
5	1.03734188611827	1.9102298103730673
6	0.6869776300307949	1.518055135540509
7	0.49505779333385663	1.2762849300451682
8	0.33599662765712507	0.9899621838075989
9	0.27038667855142806	0.8962341741862901
>10	1.830078316570976	13.299616344813996
>50	0.1878787559659455	4.800778098183406
>100	0.13916944886366334	9.734453508167357
>500	0.015905079870132954	3.9872854623133893
>1k	0.007952539935066477	4.069293720834939
>5k	0.0019881349837666193	5.140160489475712
>10k+	0.0019881349837666193	8.615260444326386
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11984	4.046311083799562	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11548	3.899098831418336	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7992	2.69844110328155	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6048	2.0420635376184704	No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT	1773	0.5986406501649385	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1625	0.5486695186226875	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1594	0.5382025924212701	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1509	0.509502956062545	No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	1235	0.41698883415324256	Illumina PCR Primer Index 10 (95% over 21bp)
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1228	0.4146253346884064	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	1108	0.37410820100550024	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1043	0.3521614202605927	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	965	0.3258252833667037	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	849	0.2866587208065611	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	835	0.28193172187688864	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	825	0.27855529406997986	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	743	0.2508685860533273	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	734	0.24782980102710933	No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG	668	0.22554537750151096	No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC	662	0.22351952081736565	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	635	0.21440316573871177	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	611	0.20629973900213053	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	607	0.20494916787936698	No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC	598	0.20191038285314902	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	559	0.18874231440620454	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	546	0.18435295825722303	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	541	0.1826647443537686	No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA	513	0.17321074649442383	No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA	495	0.1671331764419879	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	483	0.16308146307369728	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	470	0.1586921069247158	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	443	0.1495757518460619	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	429	0.14484875291638952	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	427	0.14417346735500775	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	384	0.12965482778529971	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC	350	0.11817497324180964	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	348	0.11749968768042787	No Hit
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	332	0.11209740318937371	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	327	0.11040918928591928	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT	326	0.1100715465052284	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	326	0.1100715465052284	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	307	0.1036563336721016	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.3764278069088464E-4	0.0
2	0.0	3.3764278069088464E-4	0.0	0.001012928342072654	0.0
3	0.0	3.3764278069088464E-4	0.0	0.002025856684145308	0.0
4	0.0	3.3764278069088464E-4	0.0	0.002025856684145308	0.0
5	0.0	3.3764278069088464E-4	0.0	0.002025856684145308	0.0
6	0.0	3.3764278069088464E-4	0.0	0.002025856684145308	0.0
7	0.0	3.3764278069088464E-4	0.0	0.0023634994648361928	0.0
8	0.0	3.3764278069088464E-4	0.0	0.002701142245527077	0.0
9	0.0	3.3764278069088464E-4	0.0	0.003038785026217962	0.0
10	0.0	3.3764278069088464E-4	0.0	0.004051713368290616	0.0
11	0.0	3.3764278069088464E-4	0.0	0.006415212833126809	0.0
12	0.0	6.752855613817693E-4	0.0	0.010466926201417424	0.0
13	0.0	6.752855613817693E-4	0.0	0.011817497324180963	0.0
14	0.0	6.752855613817693E-4	0.0	0.018907995718689542	0.0
15	0.0	6.752855613817693E-4	0.0	0.025660851332507235	0.0
16	0.0	6.752855613817693E-4	0.0	0.046932346516032966	0.0
17	0.0	0.001012928342072654	0.0	0.08170955292719409	0.0
18	0.0	0.001012928342072654	0.0	0.09285176468999327	0.0
19	0.0	0.001012928342072654	0.0	0.11716204489973697	0.0
20	0.0	0.001012928342072654	0.0	0.13269361281151767	0.0
21	0.0	0.001012928342072654	0.0	0.166795533661297	0.0
22	0.0	0.001012928342072654	0.0	0.24276515931674608	0.0
23	0.0	0.001012928342072654	0.0	0.33865570903295733	0.0
24	0.0	0.001012928342072654	0.0	0.5186193111411989	0.0
25	0.0	0.001012928342072654	0.0	0.5544094458944326	0.0
26	0.0	0.001012928342072654	0.0	0.6300414287691908	0.0
27	0.0	0.001012928342072654	0.0	0.6813631314342052	0.0
28	0.0	0.001012928342072654	0.0	0.7576703998703451	0.0
29	0.0	0.001012928342072654	0.0	0.8744948019893912	0.0
30	0.0	0.001012928342072654	0.0	1.0821451121142853	0.0
31	0.0	0.001012928342072654	0.0	1.6757211205688605	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGAG	20	0.0018397304	37.0	2
TTCTGAC	20	0.0018397304	37.0	26
ACTGTGC	30	3.5930853E-4	30.833334	8
AGCGTGG	25	0.005489838	29.6	18
TGTGCGC	25	0.005489838	29.6	10
GATACAC	55	6.235405E-7	26.90909	3
CCGTCTT	165	0.0	26.90909	37
TAGTACT	35	8.8537915E-4	26.42857	4
GCCGTCT	170	0.0	26.117647	36
ATGGGTC	45	1.3199744E-4	24.666666	5
AGTGGCT	70	5.0808485E-6	21.142857	27
ATACACT	70	5.0808485E-6	21.142857	4
CGTCTTC	45	0.0038192053	20.555555	37
GTGCTGG	45	0.0038192053	20.555555	1
TACACTG	45	0.0038192053	20.555555	5
GTGTATA	55	5.1307137E-4	20.181818	9
ACAGAGG	55	5.1307137E-4	20.181818	3
GAAACGC	75	9.227742E-6	19.733334	9
ATGCCCC	75	9.227742E-6	19.733334	37
TGCCGTC	230	0.0	19.304348	35
>>END_MODULE