FastQCFastQC Report
Fri 10 Feb 2017
ERR1630519.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630519.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609473
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT85181.3976008781357008No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT73021.19808424655399No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53110.8714085775743962No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37390.6134808268783031No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA23310.38246156925737484No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT22980.3770470554068843No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC20470.33586393490769895No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC20450.3355357825531238No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC19370.31781555540606393No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC18360.30124386150001725No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT18100.29697788089053984No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG17630.2892663005580231No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA17050.2797498822753428No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC16260.2667878642696231No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT15800.2592403601143939No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA15200.24939578947713845No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG14760.24217643767648445No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA14150.2321677908619414No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC14020.2300348005572027No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT13760.22576881994772532TruSeq Adapter, Index 3 (95% over 21bp)
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC13020.21362718282844356No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC12520.20542337396406404No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG12410.20361853601390054No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC12260.20115739335458666No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC11570.18983613712174288No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC10670.17506928116585968No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT9990.1639121011103035No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9990.1639121011103035No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9710.15931796814625093No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG9600.15751313019608743No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCG9510.15603644460049912No Hit
GGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT9420.1545597590049108No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGAT9350.15341122576389765No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC9200.1509500831045838No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG8950.14684817867239403No Hit
GTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCG8900.14602779778595606No Hit
GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTA8880.1456996454313809No Hit
ATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC8760.1437307313039298No Hit
GGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAG8710.14291035041749184No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG8700.14274627424020425No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT8700.14274627424020425No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8690.14258219806291664No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC8490.13930067451716482No Hit
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGA8480.13913659833987724No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC8350.13700360803513856No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGAC8170.13405023684396192No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG8060.1322453988937984No Hit
GACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGA7900.12962018005719694No Hit
ACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTT7400.12141637119281741No Hit
ATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCAC7370.12092414266095464No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG7340.12043191412909185No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG7230.11862707617892834No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT7220.11846300000164076No Hit
AGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGG7110.11665816205147726No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT7070.1160018573423269No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG7030.11534555263317653No Hit
ACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCG6950.1140329432148758No Hit
CCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTT6870.11272033379657506No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGT6850.11239218144199989No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCC6840.11222810526471229No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC6830.1120640290874247No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGC6760.11091549584641158No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC6730.11042326731454881No Hit
ACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAG6720.11025919113726121No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGC6620.1086184293643853No Hit
GGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTG6540.10730581994608457No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT6520.10697766759150938No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC6480.10632136288235901No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAA6470.10615728670507142No Hit
GCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC6380.10468060110948312No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC6370.10451652493219553No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC6210.10189130609559406No Hit
CGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCC6190.10156315374101887No Hit
CCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCG6170.10123500138644369No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCG6120.10041462050000573No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGGCC358.8645966E-426.428573
CGTCTTC659.3767085E-825.61538537
GATATAG451.3222158E-424.6666662
GCCGTCT1800.023.6388936
AGTTTAG400.001930151423.1250028
ACGATGT400.001930151423.1250028
CCGTCTT1900.022.39473737
CTTAGAA502.7000846E-422.1999992
CGTCACT1005.329639E-1022.1999991
TTTAGTA705.095437E-621.1428572
CACTGTT705.095437E-621.1428572
CAGACCA450.003823776520.5555554
AGCGTCA555.139286E-420.1818183
TTGTATC656.8953326E-519.9230774
TTCTATA951.6728882E-719.4736842
CATATAA701.21800535E-418.52
TATGCCG2300.018.533
GTTGTAT609.230029E-418.53
GCAGCGT609.230029E-418.51
CGCCGTA609.230029E-418.525
Produced by FastQC (version 0.11.5)