FastQCFastQC Report
Fri 10 Feb 2017
ERR1630518.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630518.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2036843
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC82970.40734607429242214No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT54430.26722727279422126No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT48200.23664072292267987No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT47770.2345296127389298No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43840.21523504757116774No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33110.16255548414875373No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG31570.15499476395578843No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT30280.1486614334045383No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG28690.14085523528323No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT27560.13530743410267754No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG25480.12509555228360753No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG25440.12489916994093311No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG24570.12062785398776439No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT24320.11940046434604926No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT22450.11021958982601998No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT22080.10840305315628156No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC22000.10801028847093272No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG21610.10609556062985709No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTTA701.2197841E-418.519
GGTATCA28100.018.1049821
TGCATCA20850.017.39088614
ACAGGCT22050.017.1156448
GACAGGC21900.017.0639277
CCAGGAC24150.016.9296073
GTTATAC1107.814888E-716.8181823
GCATCAG21550.016.74013915
CAGGACA25750.016.5242734
GCTGCAT22100.016.3235312
AAGACGG3200.016.18755
CTGCATC22750.016.18241713
GGCCATC23450.015.93603426
ATCAAGC22900.015.91484630
TCCAGGA25650.015.8674462
CTTATAC14500.015.56551637
AGAGGCC23550.015.47558423
AGGCCAT24000.015.26249925
CCATCAA24000.015.18541528
TCTAGCG2700.015.07407328
Produced by FastQC (version 0.11.5)