FastQCFastQC Report
Fri 10 Feb 2017
ERR1630517.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630517.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1520585
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA20250.13317243034753073No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC18400.12100606016763285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11050.021.9321271
TCTAATA1153.0522642E-919.3043462
TCGTTTA2150.018.93023330
TAATACT2350.017.3191494
AAGACGG3000.017.2666665
CTTATAC6800.017.13970637
GTACTAG650.00158025117.0769231
GCGAACT803.3834644E-416.187528
GAACCGT803.3834644E-416.18756
CGAACGA2300.016.08695616
GACGGAC3250.015.9384617
CAAGACG3450.015.5507254
CATCGTT2650.015.35849128
ACGGACC3400.015.2352948
GTATAGA1101.452403E-515.1363641
TCGCCTA750.004105216414.835
CGCATCG2750.014.813
CGAGCCG4750.014.815
CGCAAGA3500.014.7999992
CGGACCA3500.014.7999999
Produced by FastQC (version 0.11.5)