##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630517.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1520585
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.969261830150895	33.0	31.0	34.0	30.0	34.0
2	32.14450819914704	33.0	31.0	34.0	30.0	34.0
3	32.25635002318187	34.0	31.0	34.0	30.0	34.0
4	35.841951617305185	37.0	35.0	37.0	35.0	37.0
5	35.8147890450057	37.0	35.0	37.0	35.0	37.0
6	35.84867994883548	37.0	35.0	37.0	35.0	37.0
7	35.82747955556579	37.0	35.0	37.0	35.0	37.0
8	35.812258440008286	37.0	35.0	37.0	35.0	37.0
9	37.558723123008576	39.0	37.0	39.0	35.0	39.0
10	37.450602892965534	39.0	37.0	39.0	34.0	39.0
11	37.56267554921297	39.0	37.0	39.0	35.0	39.0
12	37.47828302922888	39.0	37.0	39.0	35.0	39.0
13	37.52369252623168	39.0	37.0	39.0	35.0	39.0
14	38.80397084017007	40.0	38.0	41.0	35.0	41.0
15	38.78617242705932	40.0	38.0	41.0	35.0	41.0
16	38.7672330057182	40.0	38.0	41.0	35.0	41.0
17	38.73984025884775	40.0	38.0	41.0	35.0	41.0
18	38.71359904247378	40.0	38.0	41.0	35.0	41.0
19	38.734737617430135	40.0	38.0	41.0	34.0	41.0
20	38.72120992907335	40.0	38.0	41.0	34.0	41.0
21	38.64392585748248	40.0	38.0	41.0	34.0	41.0
22	38.5909344101119	40.0	38.0	41.0	34.0	41.0
23	38.563719226481915	40.0	38.0	41.0	34.0	41.0
24	38.516932627903074	40.0	38.0	41.0	34.0	41.0
25	38.48099580095818	40.0	38.0	41.0	34.0	41.0
26	38.30238822558423	40.0	38.0	41.0	34.0	41.0
27	38.19518737854181	40.0	38.0	41.0	33.0	41.0
28	38.092059963763944	40.0	38.0	41.0	33.0	41.0
29	38.00684078824926	40.0	38.0	41.0	33.0	41.0
30	37.95131018654005	40.0	37.0	41.0	33.0	41.0
31	37.93246283502731	40.0	37.0	41.0	33.0	41.0
32	37.84715422025076	40.0	37.0	41.0	33.0	41.0
33	37.819184064027986	40.0	37.0	41.0	33.0	41.0
34	37.76478131771653	40.0	37.0	41.0	33.0	41.0
35	37.71965394897359	40.0	37.0	41.0	33.0	41.0
36	37.702784783487935	40.0	37.0	41.0	33.0	41.0
37	37.53801990681218	40.0	37.0	41.0	32.0	41.0
38	37.43534231891016	40.0	37.0	41.0	32.0	41.0
39	37.4036190018973	40.0	36.0	41.0	32.0	41.0
40	37.32246536694759	40.0	36.0	41.0	31.0	41.0
41	37.223284459599434	39.0	36.0	41.0	31.0	41.0
42	37.161227422340744	39.0	36.0	41.0	31.0	41.0
43	36.28463190153789	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	1.0
12	1.0
13	2.0
14	0.0
15	2.0
16	4.0
17	23.0
18	48.0
19	121.0
20	219.0
21	386.0
22	773.0
23	1277.0
24	2138.0
25	3381.0
26	5268.0
27	7536.0
28	11032.0
29	15713.0
30	21225.0
31	28629.0
32	36986.0
33	48058.0
34	64059.0
35	86585.0
36	121835.0
37	183587.0
38	344703.0
39	536986.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.65201550718967	18.334391040290416	11.746597526609824	26.26699592591009
2	19.65414626607523	20.254638839657105	33.6835494234127	26.40766547085497
3	20.784500702032442	22.134770499511703	28.656799850057705	28.423928948398146
4	14.95075908285298	14.828569267748925	33.250821229987146	36.969850419410946
5	15.134832975466677	36.22921441418928	33.727479884386604	14.908472725957445
6	36.816948740123046	33.51644268488773	14.92333542682586	14.743273148163372
7	30.466958440337105	29.390662146476522	20.41437999191101	19.727999421275364
8	27.72242262024155	32.67143895277147	19.49775908614119	20.10837934084579
9	27.00362031718056	13.451138870895083	18.655188628060912	40.890052183863446
10	17.39271398836632	25.937451704442697	32.23042447479095	24.43940983240003
11	37.27203674901436	20.75017180887619	20.493954629303854	21.4838368128056
12	22.586701828572554	24.703387183222247	27.6354166324145	25.074494355790698
13	30.628672517485043	18.85833412798364	24.551669258870763	25.961324095660554
14	23.824120322112872	20.27522302271823	23.514239585422718	32.38641706974618
15	26.796528967469758	26.936277814130744	21.12759234110556	25.139600877293937
16	27.089376785908055	25.319071278488213	22.36461624966707	25.226935685936663
17	25.155121219793696	25.470526146187154	23.79268505213454	25.581667581884602
18	24.82097350690688	23.979192218784217	25.572920948187704	25.626913326121198
19	25.61172180443711	24.52135197966572	24.70943748623063	25.157488729666543
20	26.035177250860684	23.621435171332088	24.83083813137707	25.512549446430157
21	26.494737222845156	24.467622658384766	23.78334654096943	25.254293577800652
22	26.315595642466548	23.9941864479789	24.0415366454358	25.648681264118743
23	25.29046386752467	24.283680294097337	24.60901560912412	25.816840229253874
24	25.74226366825926	24.833337169576183	24.20193543932105	25.222463722843514
25	25.986643298467367	24.245471315316145	24.207262336534953	25.560623049681535
26	25.81624835178566	25.07008815686068	24.120650933686704	24.99301255766695
27	26.430288342973263	23.697458543915666	23.969656415129702	25.902596697981366
28	25.11888516590654	24.130120973178084	24.947174935962146	25.803818924953227
29	24.72239302636814	24.71088429781959	24.959341306142043	25.60738136967022
30	24.571069686995465	25.59593840528481	24.861484231397785	24.97150767632194
31	25.731609873831452	25.06601077874634	23.86542021656139	25.33695913086082
32	24.790787756028106	24.57975055652923	24.255204411460063	26.3742572759826
33	24.67228073405959	24.249219872614816	24.833731754554993	26.244767638770604
34	25.68143181735977	23.954662185935018	24.827944508199145	25.53596148850607
35	25.63875087548542	23.95426760095621	25.447179868274382	24.959801655283986
36	24.568636412959485	25.041414981734007	24.852606069374616	25.537342535931895
37	25.845776461033086	23.812545829401184	24.821039271070017	25.520638438495713
38	24.802099192087258	23.847466600025648	25.795598404561403	25.554835803325695
39	25.24429742500419	23.64287428851396	25.420282325552336	25.692545960929507
40	24.404423297612432	23.593288109510485	26.391684779213264	25.61060381366382
41	23.818398839920164	23.786700513289293	26.933449955115957	25.461450691674585
42	24.848397162934003	23.39849465830585	26.729778341888156	25.023329836871994
43	23.881992785671304	22.815561116280904	26.848088071367272	26.45435802668052
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	34.5
2	48.0
3	120.0
4	192.0
5	192.0
6	262.0
7	332.0
8	358.5
9	385.0
10	574.0
11	763.0
12	763.0
13	1313.5
14	1864.0
15	2793.0
16	3722.0
17	3848.5
18	3975.0
19	3975.0
20	5042.0
21	6109.0
22	6850.5
23	7592.0
24	9402.0
25	11212.0
26	11212.0
27	13760.0
28	16308.0
29	21416.0
30	26524.0
31	30837.5
32	35151.0
33	35151.0
34	39960.5
35	44770.0
36	50704.5
37	56639.0
38	65672.5
39	74706.0
40	74706.0
41	80032.5
42	85359.0
43	85731.0
44	86103.0
45	91202.5
46	96302.0
47	96302.0
48	102426.0
49	108550.0
50	112182.5
51	115815.0
52	123627.0
53	131439.0
54	131439.0
55	123828.0
56	116217.0
57	111290.0
58	106363.0
59	99488.0
60	92613.0
61	92613.0
62	86794.5
63	80976.0
64	72853.5
65	64731.0
66	57476.0
67	50221.0
68	50221.0
69	42539.5
70	34858.0
71	30276.5
72	25695.0
73	20027.5
74	14360.0
75	14360.0
76	11288.5
77	8217.0
78	6853.5
79	5490.0
80	4289.5
81	3089.0
82	3089.0
83	2297.0
84	1505.0
85	1303.5
86	1102.0
87	926.0
88	750.0
89	750.0
90	595.5
91	441.0
92	247.5
93	54.0
94	35.0
95	16.0
96	16.0
97	10.0
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1520585.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.351888277532126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.95996750794939	49.00358373625788
2	7.996204687454117	9.012024663833863
3	1.982296563469409	3.3511846343269203
4	0.8384697960705946	1.8899742508902115
5	0.48316498766008703	1.3613629702118206
6	0.30131030405085113	1.018764275444568
7	0.20089453058690943	0.7924550300240547
8	0.13261740672451705	0.5978593029916818
9	0.11516958256324791	0.5841021104616744
>10	0.7375667299793768	8.293715948802458
>50	0.11369416975361647	4.535221049639611
>100	0.12634852939894953	14.231872110608865
>500	0.010187455023808538	3.7985773856679845
>1k	0.0021077493152707317	1.5293025308383632
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2025	0.13317243034753073	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1840	0.12100606016763285	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.918774682112476E-4	0.0
3	0.0	0.0	0.0	7.891699576149969E-4	0.0
4	0.0	0.0	0.0	0.0013152832626916614	0.0
5	0.0	0.0	0.0	0.0013810474258262445	0.0
6	0.0	0.0	0.0	0.0015125757520954106	0.0
7	0.0	0.0	0.0	0.001775632404633743	0.0
8	0.0	0.0	0.0	0.0019071607309029091	0.0
9	0.0	0.0	0.0	0.0034855006461329027	0.0
10	0.0	0.0	0.0	0.0048007839088245645	0.0
11	0.0	0.0	0.0	0.005655718029574144	0.0
12	0.0	0.0	0.0	0.006379123824054558	0.0
13	0.0	0.0	0.0	0.00664218047659289	0.0
14	0.0	0.0	0.0	0.007036765455400389	0.0
15	0.0	0.0	0.0	0.0076286429236116365	0.0
16	0.0	0.0	0.0	0.008549341207495799	0.0
17	0.0	0.0	0.0	0.010127681122725793	0.0
18	0.0	6.576416313458307E-5	0.0	0.010588030264667874	0.0
19	6.576416313458307E-5	6.576416313458307E-5	0.0	0.011508728548552038	0.0
20	6.576416313458307E-5	6.576416313458307E-5	0.0	0.012166370179897868	0.0
21	6.576416313458307E-5	6.576416313458307E-5	0.0	0.014468115889608276	0.0
22	6.576416313458307E-5	6.576416313458307E-5	0.0	0.016769861599318682	0.0
23	6.576416313458307E-5	6.576416313458307E-5	0.0	0.019992305592913255	0.0
24	6.576416313458307E-5	1.3152832626916615E-4	0.0	0.024464268686064904	0.0
25	6.576416313458307E-5	1.3152832626916615E-4	0.0	0.02617413692756406	0.0
26	6.576416313458307E-5	1.3152832626916615E-4	0.0	0.029462345084293216	0.0
27	6.576416313458307E-5	1.9729248940374922E-4	0.0	0.036499110539693604	0.0
28	6.576416313458307E-5	3.2882081567291535E-4	0.0	0.057872463558433104	0.0
29	6.576416313458307E-5	3.2882081567291535E-4	0.0	0.08865009190541798	0.0
30	6.576416313458307E-5	3.2882081567291535E-4	0.0	0.1332381945106653	0.0
31	6.576416313458307E-5	3.2882081567291535E-4	0.0	0.2925189976226255	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1105	0.0	21.932127	1
TCTAATA	115	3.0522642E-9	19.304346	2
TCGTTTA	215	0.0	18.930233	30
TAATACT	235	0.0	17.319149	4
AAGACGG	300	0.0	17.266666	5
CTTATAC	680	0.0	17.139706	37
GTACTAG	65	0.001580251	17.076923	1
GCGAACT	80	3.3834644E-4	16.1875	28
GAACCGT	80	3.3834644E-4	16.1875	6
CGAACGA	230	0.0	16.086956	16
GACGGAC	325	0.0	15.938461	7
CAAGACG	345	0.0	15.550725	4
CATCGTT	265	0.0	15.358491	28
ACGGACC	340	0.0	15.235294	8
GTATAGA	110	1.452403E-5	15.136364	1
TCGCCTA	75	0.0041052164	14.8	35
CGCATCG	275	0.0	14.8	13
CGAGCCG	475	0.0	14.8	15
CGCAAGA	350	0.0	14.799999	2
CGGACCA	350	0.0	14.799999	9
>>END_MODULE