FastQCFastQC Report
Fri 10 Feb 2017
ERR1630516.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630516.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1481373
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC43660.2947265813539196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT34990.23619979573004232No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29800.2011647302873753No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26970.18206083140437956No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT25020.16889736750973589No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20410.13777758876393723No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG17090.11536594767151825No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT16940.11435337352577643No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG15120.10206747389077564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC556.263799E-726.9090931
GGTATCA14750.023.203391
GTACTAT753.7422797E-722.21
TCGTATG603.7272217E-521.58333430
GCGTAGA450.003826321320.5555571
TCGGGTC759.268737E-619.73333437
TACACGG609.2385325E-418.55
TGCATCA11650.017.15021314
ATAGGCG650.001580225317.07692332
CTTATAC7700.017.05844137
GCATCAG12050.016.7344415
CGTCTAT904.44735E-516.4444451
GACAGGC12400.015.9637097
GGCCATC13150.015.75665426
CCAGGAC15050.015.3654483
CTGCATC13350.015.24344613
ACAGGCT13750.015.2036368
TCCAGGA16150.014.891642
ATCAAGC13200.014.85606130
CTCGTAT750.004105150214.829
Produced by FastQC (version 0.11.5)