FastQCFastQC Report
Fri 10 Feb 2017
ERR1630515.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630515.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173674
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14850.12652576439454227No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13230.11272295373331946No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA13170.11221173852364455No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCA250.005496391629.5999984
GAACCGT604.3230102E-827.7500026
GGTATCA8700.021.9022981
CAAGACG2750.019.509094
ACGGACC2900.018.58
GTCGGTT1054.7974754E-717.6190512
GACGGAC3150.017.6190477
TTAGAGT1705.456968E-1217.4117644
AAGACGG3200.017.343755
CGGACCA3100.017.3064529
TGCGTTA650.00157996117.07692313
AGACGGA3400.016.323536
GTATAAC1151.2421733E-616.0869561
CGCAAGA3150.015.8571422
TAGAACA3750.015.7866684
TTTCGAG1701.4861143E-915.23529412
TCGCCAT2800.015.19642913
ATTCGGA1101.4518408E-515.13636429
ATACTAT1101.4518408E-515.1363646
GTATCAA12850.014.82879452
Produced by FastQC (version 0.11.5)