##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630515.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1173674
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.664919730691828	31.0	31.0	34.0	30.0	34.0
2	31.844387794225653	33.0	31.0	34.0	30.0	34.0
3	31.946104284494673	33.0	31.0	34.0	30.0	34.0
4	35.59828112406	37.0	35.0	37.0	33.0	37.0
5	35.55222744987109	37.0	35.0	37.0	33.0	37.0
6	35.58171434316514	37.0	35.0	37.0	33.0	37.0
7	35.562041077846146	37.0	35.0	37.0	33.0	37.0
8	35.54659726636187	37.0	35.0	37.0	33.0	37.0
9	37.22725049715679	39.0	37.0	39.0	34.0	39.0
10	37.077017127413576	39.0	37.0	39.0	33.0	39.0
11	37.21286745723259	39.0	37.0	39.0	34.0	39.0
12	37.12236362056244	39.0	37.0	39.0	33.0	39.0
13	37.178193433611035	39.0	37.0	39.0	33.0	39.0
14	38.38143641249614	40.0	38.0	41.0	34.0	41.0
15	38.345215962865325	40.0	38.0	41.0	34.0	41.0
16	38.3300780284815	40.0	38.0	41.0	34.0	41.0
17	38.291016926335594	40.0	38.0	41.0	33.0	41.0
18	38.25716510717627	40.0	38.0	41.0	33.0	41.0
19	38.27635527412212	40.0	38.0	41.0	34.0	41.0
20	38.256823445011136	40.0	38.0	41.0	34.0	41.0
21	38.17938030492283	40.0	38.0	41.0	33.0	41.0
22	38.11682971591771	40.0	38.0	41.0	33.0	41.0
23	38.083697858178674	40.0	37.0	41.0	33.0	41.0
24	38.02691718484008	40.0	37.0	41.0	33.0	41.0
25	37.98243294134487	40.0	37.0	41.0	33.0	41.0
26	37.77176711761528	40.0	37.0	41.0	33.0	41.0
27	37.65028789936558	40.0	37.0	41.0	32.0	41.0
28	37.53783929779479	40.0	37.0	41.0	32.0	41.0
29	37.43211232420587	39.0	37.0	41.0	32.0	41.0
30	37.370010752559914	39.0	37.0	41.0	31.0	41.0
31	37.33753239826391	39.0	36.0	41.0	31.0	41.0
32	37.24951477156348	39.0	36.0	41.0	31.0	41.0
33	37.22785628718026	39.0	36.0	41.0	31.0	41.0
34	37.15487775992311	39.0	36.0	41.0	31.0	41.0
35	37.11446193747156	39.0	36.0	41.0	31.0	41.0
36	37.09510562558257	39.0	36.0	41.0	31.0	41.0
37	36.90877364583351	39.0	36.0	40.0	30.0	41.0
38	36.79693339036223	39.0	35.0	40.0	30.0	41.0
39	36.76015145602612	39.0	35.0	40.0	30.0	41.0
40	36.67292962100208	39.0	35.0	40.0	30.0	41.0
41	36.562271124690504	39.0	35.0	40.0	30.0	41.0
42	36.495514938560454	39.0	35.0	40.0	30.0	41.0
43	35.57536164215958	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	3.0
12	2.0
13	1.0
14	3.0
15	4.0
16	17.0
17	20.0
18	60.0
19	109.0
20	238.0
21	434.0
22	824.0
23	1490.0
24	2314.0
25	3826.0
26	5506.0
27	8125.0
28	11429.0
29	15803.0
30	20864.0
31	27278.0
32	34595.0
33	44847.0
34	58416.0
35	78393.0
36	109632.0
37	166515.0
38	306742.0
39	276183.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.96857560106128	19.404536523770656	12.10037881047037	25.526509064697695
2	19.566932555377388	21.012223155663325	33.95355098604894	25.46729330291035
3	20.803902957720798	23.11723698403475	29.009418288212913	27.069441770031546
4	15.002888365934663	15.373008177739303	33.97101750571283	35.6530859506132
5	14.682867644678163	36.763700993631964	34.111516485838486	14.441914875851387
6	35.86992640205031	34.477461373430785	15.127624877095345	14.52498734742356
7	29.49532834500892	30.004583896380083	21.13670405921917	19.363383699391825
8	27.20746987664377	33.214930210603626	19.868890339225374	19.70870957352723
9	26.67282396985875	13.62414094544141	19.353755812942946	40.34927927175689
10	17.011367722212473	26.440732264666337	32.81763079015127	23.73026922296992
11	36.55427316273514	21.194897390587165	21.17768647852811	21.07314296814959
12	22.127779945708944	25.22054676170725	28.18908828175456	24.46258501082924
13	30.042499024430974	19.60442167075355	25.00958528518141	25.343494019634072
14	23.211300582614932	20.85868818769096	24.30589754906388	31.624113680630227
15	26.06933441483751	27.770658632635637	21.784498932412237	24.375508020114616
16	26.29409870202458	26.12880578422969	23.083070767521473	24.494024746224248
17	24.396808653851068	26.028522400598465	24.56874736937173	25.005921576178736
18	24.047819070712993	24.64628167617243	26.244681231756005	25.061218021358574
19	24.79138159318516	25.19191870996546	25.656357727955122	24.360341968894257
20	25.34272719681956	24.334781208410515	25.694784071215686	24.62770752355424
21	25.69188718502753	25.22182479973144	24.67942546226635	24.406862552974676
22	25.522845355695022	24.779794048432528	24.96221267575153	24.73514792012092
23	24.53193987427514	24.930346927681793	25.506316063915534	25.031397134127538
24	25.110209478952417	25.402709781421418	25.01810553867599	24.46897520095018
25	25.295951005134302	24.810040948338294	25.148891429817823	24.74511661670958
26	24.952755194372543	25.613671257947267	25.026455387100675	24.407118160579515
27	25.52088569739127	24.6449184356133	24.823332543789842	25.01086332320559
28	24.369799450273245	24.96101984026229	25.783905922769012	24.885274786695454
29	24.184569139301033	25.410378009566543	25.628155688888054	24.77689716224437
30	24.065796805586558	26.077769465797147	25.602509725869364	24.253924002746928
31	24.987943841305167	25.588280902533413	24.873602039407878	24.550173216753546
32	24.1729815945484	25.221057976916928	25.174707797906404	25.431252630628265
33	24.126375807933037	24.892772609770685	25.677487956621686	25.303363625674592
34	24.832704822633882	24.708138716543093	25.63352344858964	24.825633012233382
35	24.887916065278773	24.789421934881407	26.06626712357946	24.25639487626036
36	24.087097439323017	25.593563459700054	25.537500191705703	24.78183890927123
37	25.163972278503234	24.451934693961014	25.564679800353417	24.819413227182334
38	24.134384846217944	24.635290549164417	26.398812617472995	24.83151198714464
39	24.48260760654151	24.450912263541667	26.04138798337528	25.025092146541546
40	23.921804521528127	24.365880133665737	26.94410884112624	24.7682065036799
41	23.244273963638967	24.638443042957412	27.37199597162415	24.745287021779472
42	24.242847673203975	24.221121026792787	27.12473821521138	24.411293084791858
43	23.325983194652007	23.76903637637027	27.273927853901508	25.631052575076215
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	46.0
1	47.5
2	49.0
3	142.0
4	235.0
5	235.0
6	308.5
7	382.0
8	409.5
9	437.0
10	683.0
11	929.0
12	929.0
13	1514.5
14	2100.0
15	3132.5
16	4165.0
17	4486.0
18	4807.0
19	4807.0
20	6021.0
21	7235.0
22	8238.0
23	9241.0
24	10910.0
25	12579.0
26	12579.0
27	14829.0
28	17079.0
29	21730.0
30	26381.0
31	29414.0
32	32447.0
33	32447.0
34	36747.0
35	41047.0
36	45300.0
37	49553.0
38	56137.5
39	62722.0
40	62722.0
41	66405.5
42	70089.0
43	69687.5
44	69286.0
45	72065.0
46	74844.0
47	74844.0
48	78460.5
49	82077.0
50	83644.5
51	85212.0
52	90029.5
53	94847.0
54	94847.0
55	89047.0
56	83247.0
57	79241.0
58	75235.0
59	70477.0
60	65719.0
61	65719.0
62	61475.0
63	57231.0
64	51176.0
65	45121.0
66	39723.0
67	34325.0
68	34325.0
69	29106.5
70	23888.0
71	20741.5
72	17595.0
73	13646.5
74	9698.0
75	9698.0
76	7500.0
77	5302.0
78	4356.0
79	3410.0
80	2748.0
81	2086.0
82	2086.0
83	1599.0
84	1112.0
85	964.0
86	816.0
87	717.0
88	618.0
89	618.0
90	508.5
91	399.0
92	232.5
93	66.0
94	39.0
95	12.0
96	12.0
97	7.0
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1173674.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.97610804969259
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.82664983195417	53.27476748013492
2	6.7470074761550185	8.093184988039143
3	1.673074591368055	3.0103350740125725
4	0.7082793411967744	1.699193531879313
5	0.4003775439980901	1.200654341975
6	0.25425644591259516	0.9149587243430778
7	0.18895339314545467	0.7932882386553483
8	0.14131947891127672	0.678063386936719
9	0.10453522233536097	0.5642654210805824
>10	0.71413086549621	8.730556534936312
>50	0.1216565501391883	5.192089333685265
>100	0.11292401193571146	12.810645815146804
>500	0.005696039543486308	2.222108158524487
>1k	0.0011392079086972617	0.815888970650489
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1485	0.12652576439454227	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1323	0.11272295373331946	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1317	0.11221173852364455	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	8.520253494581971E-5	0.0	7.668228145123774E-4	0.0
3	0.0	8.520253494581971E-5	0.0	0.0012780380241872956	0.0
4	0.0	8.520253494581971E-5	0.0	0.0022152659085913125	0.0
5	0.0	8.520253494581971E-5	0.0	0.002641278583320411	0.0
6	8.520253494581971E-5	8.520253494581971E-5	0.0	0.002641278583320411	0.0
7	8.520253494581971E-5	8.520253494581971E-5	0.0	0.00289688618815787	0.0
8	8.520253494581971E-5	8.520253494581971E-5	0.0	0.003237696327941149	0.0
9	8.520253494581971E-5	8.520253494581971E-5	0.0	0.004515734352128445	0.0
10	8.520253494581971E-5	8.520253494581971E-5	0.0	0.0063049875859906585	0.0
11	8.520253494581971E-5	8.520253494581971E-5	0.0	0.006986607865557216	0.0
12	8.520253494581971E-5	8.520253494581971E-5	0.0	0.007071810400503036	0.0
13	8.520253494581971E-5	8.520253494581971E-5	0.0	0.007412620540286315	0.0
14	8.520253494581971E-5	8.520253494581971E-5	0.0	0.007668228145123774	0.0
15	8.520253494581971E-5	8.520253494581971E-5	0.0	0.008349848424690332	0.0
16	8.520253494581971E-5	1.7040506989163942E-4	0.0	0.00886106363436525	0.0
17	8.520253494581971E-5	1.7040506989163942E-4	0.0	0.010139101658552545	0.0
18	8.520253494581971E-5	1.7040506989163942E-4	0.0	0.010479911798335824	0.0
19	8.520253494581971E-5	1.7040506989163942E-4	0.0	0.01158754475263148	0.0
20	8.520253494581971E-5	1.7040506989163942E-4	0.0	0.013291595451547875	0.0
21	8.520253494581971E-5	1.7040506989163942E-4	0.0	0.01456963347573517	0.0
22	8.520253494581971E-5	1.7040506989163942E-4	0.0	0.01721091205905558	0.0
23	8.520253494581971E-5	2.556076048374591E-4	0.0	0.021300633736454928	0.0
24	8.520253494581971E-5	2.556076048374591E-4	0.0	0.027009203577824846	0.0
25	8.520253494581971E-5	2.556076048374591E-4	0.0	0.028457646671903782	0.0
26	8.520253494581971E-5	2.556076048374591E-4	0.0	0.03212135567457403	0.0
27	8.520253494581971E-5	2.556076048374591E-4	0.0	0.03782992551594395	0.0
28	8.520253494581971E-5	2.556076048374591E-4	0.0	0.05521124264489117	0.0
29	8.520253494581971E-5	4.260126747290985E-4	0.0	0.0825612563624993	0.0
30	8.520253494581971E-5	4.260126747290985E-4	0.0	0.12218043511230546	0.0
31	8.520253494581971E-5	4.260126747290985E-4	0.0	0.2765674284341308	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGCA	25	0.0054963916	29.599998	4
GAACCGT	60	4.3230102E-8	27.750002	6
GGTATCA	870	0.0	21.902298	1
CAAGACG	275	0.0	19.50909	4
ACGGACC	290	0.0	18.5	8
GTCGGTT	105	4.7974754E-7	17.61905	12
GACGGAC	315	0.0	17.619047	7
TTAGAGT	170	5.456968E-12	17.411764	4
AAGACGG	320	0.0	17.34375	5
CGGACCA	310	0.0	17.306452	9
TGCGTTA	65	0.001579961	17.076923	13
AGACGGA	340	0.0	16.32353	6
GTATAAC	115	1.2421733E-6	16.086956	1
CGCAAGA	315	0.0	15.857142	2
TAGAACA	375	0.0	15.786668	4
TTTCGAG	170	1.4861143E-9	15.235294	12
TCGCCAT	280	0.0	15.196429	13
ATTCGGA	110	1.4518408E-5	15.136364	29
ATACTAT	110	1.4518408E-5	15.136364	6
GTATCAA	1285	0.0	14.8287945	2
>>END_MODULE