FastQCFastQC Report
Fri 10 Feb 2017
ERR1630514.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630514.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1516469
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT85800.5657880246810189No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT76060.5015598736274859No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT65800.4339027042425529No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51690.34085761067321524No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15930.10504665772923812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTA806.963128E-720.81255
CGAATTA1351.1532393E-917.81481615
AACCGCG953.6079236E-617.5263167
GTCTAAT1005.88028E-616.6500021
TCTAGCG1452.9813236E-916.58620628
GGTATCA45950.016.386291
GTATTGG1606.2937033E-1016.18751
ACGGTAT1851.8189894E-1116.09
ATACCGC2003.6379788E-1215.72527
ACGACGG1902.7284841E-1115.5789486
AGAACCG1455.3485564E-815.3103455
TACGACG1954.1836756E-1115.1794865
CTAGCGG1601.0970325E-815.0312529
CGACGGT2006.184564E-1114.87
GACGGTA2006.184564E-1114.88
CGGTCCA1904.5474735E-1014.60526410
CCTACAC2301.8189894E-1214.4782613
CGCAATA1803.3323886E-914.38888936
AATCGGA908.276161E-414.38888927
CTTGCGC2450.014.346943
Produced by FastQC (version 0.11.5)