##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630514.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1516469
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.979838031637968	33.0	31.0	34.0	30.0	34.0
2	32.14162900791246	33.0	31.0	34.0	30.0	34.0
3	32.23958089482871	34.0	31.0	34.0	30.0	34.0
4	35.825931819245895	37.0	35.0	37.0	35.0	37.0
5	35.80694692736878	37.0	35.0	37.0	35.0	37.0
6	35.84565329063766	37.0	35.0	37.0	35.0	37.0
7	35.82125186865014	37.0	35.0	37.0	35.0	37.0
8	35.82565551949958	37.0	35.0	37.0	35.0	37.0
9	37.53866383025304	39.0	37.0	39.0	35.0	39.0
10	37.453595820290424	39.0	37.0	39.0	34.0	39.0
11	37.56067878736723	39.0	37.0	39.0	35.0	39.0
12	37.5030389674962	39.0	37.0	39.0	35.0	39.0
13	37.54819320408132	39.0	37.0	39.0	35.0	39.0
14	38.81394476247124	40.0	38.0	41.0	35.0	41.0
15	38.78111323080129	40.0	38.0	41.0	35.0	41.0
16	38.75826212075552	40.0	38.0	41.0	35.0	41.0
17	38.75954404607018	40.0	38.0	41.0	35.0	41.0
18	38.75429566974333	40.0	38.0	41.0	35.0	41.0
19	38.79255692005574	40.0	38.0	41.0	35.0	41.0
20	38.774542044710444	40.0	38.0	41.0	35.0	41.0
21	38.70622610814992	40.0	38.0	41.0	34.0	41.0
22	38.642310525305824	40.0	38.0	41.0	34.0	41.0
23	38.618027800106695	40.0	38.0	41.0	34.0	41.0
24	38.56169430433461	40.0	38.0	41.0	34.0	41.0
25	38.51735050304358	40.0	38.0	41.0	34.0	41.0
26	38.31135948047734	40.0	38.0	41.0	34.0	41.0
27	38.18751454859941	40.0	38.0	41.0	34.0	41.0
28	38.092102113528206	40.0	37.0	41.0	33.0	41.0
29	37.976748618006695	40.0	37.0	41.0	33.0	41.0
30	37.882780986620894	40.0	37.0	41.0	33.0	41.0
31	37.80824204121548	40.0	37.0	41.0	33.0	41.0
32	37.69286150920329	40.0	37.0	41.0	33.0	41.0
33	37.64239625076411	40.0	37.0	41.0	33.0	41.0
34	37.544238622747976	40.0	36.0	41.0	33.0	41.0
35	37.47131593194454	40.0	36.0	41.0	32.0	41.0
36	37.39952811432347	40.0	36.0	41.0	32.0	41.0
37	37.18963592397866	40.0	36.0	41.0	31.0	41.0
38	37.053112856246976	40.0	36.0	41.0	31.0	41.0
39	36.95315235590045	40.0	36.0	41.0	31.0	41.0
40	36.818777040612105	39.0	35.0	41.0	31.0	41.0
41	36.661013842023806	39.0	35.0	41.0	30.0	41.0
42	36.53726057044358	39.0	35.0	41.0	30.0	41.0
43	35.70696928193059	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	4.0
11	5.0
12	5.0
13	4.0
14	3.0
15	5.0
16	9.0
17	29.0
18	39.0
19	102.0
20	217.0
21	485.0
22	863.0
23	1446.0
24	2343.0
25	3918.0
26	5991.0
27	8701.0
28	12777.0
29	17615.0
30	23650.0
31	30776.0
32	39179.0
33	50499.0
34	65971.0
35	87721.0
36	120920.0
37	187140.0
38	325323.0
39	530723.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.50052655214185	19.95748017269064	12.552976684653627	23.989016590513884
2	18.471528267310443	21.731535560568663	34.99174727607357	24.80518889604733
3	20.750704432467792	24.310421116422425	29.35312228604739	25.58575216506239
4	14.526838332995926	16.038573818521844	34.22984577990055	35.20474206858169
5	13.928013035545073	37.75856941355214	34.04672301247174	14.266694538431052
6	33.88832874262514	34.41204535008629	16.257371565129258	15.44225434215932
7	28.08504493003154	31.253787581546344	22.131082138836998	18.53008534958512
8	27.080936042873283	33.3389604403387	20.592837703902948	18.987265812885067
9	26.62830562312847	13.942256650152427	21.151174207979192	38.27826351873991
10	17.338633364744023	27.426343697101625	32.92846738047398	22.306555557680376
11	33.73257217918731	22.64556677386745	22.02570576780666	21.59615527913858
12	21.010782284372446	26.71297599884996	29.701958958607133	22.57428275817046
13	29.227171805028657	20.75512259070248	26.4981348118557	23.519570792413166
14	22.520671375412224	22.069161980891135	26.755574957351584	28.654591686345054
15	25.33576354017128	27.794633454426037	24.014932055980044	22.85467094942264
16	23.91733691885558	27.03016019450447	25.912893702410006	23.139609184229943
17	22.432967637320644	27.41909000447751	27.15749547138781	22.990446886814038
18	22.18996893441277	25.665806554568544	28.820371534136207	23.32385297688248
19	22.757933066881026	26.411024557706092	28.94895972156371	21.88208265384917
20	23.381157148612992	25.53609734191731	28.860794384850596	22.2219511246191
21	23.72874091062857	25.90280447539646	27.950324075203646	22.418130538771315
22	23.59817444339449	25.86877806272334	27.99470348553119	22.53834400835098
23	22.91507442618346	26.053021855375874	28.25629801862089	22.775605699819778
24	22.70221151899577	26.630349845595262	28.131006964204346	22.53643167120462
25	23.01682395090173	26.142044446671843	28.219502014218556	22.62162958820787
26	22.731028461511578	26.783666530604975	28.09018845752864	22.395116550354803
27	22.94494645126277	26.523852449341202	27.685564294423425	22.845636804972607
28	22.360892309701022	26.535260529559128	28.48551470554294	22.618332455196906
29	21.956663802557124	26.884360972759747	28.766034782115558	22.392940442567568
30	22.04107040763774	27.298876534897843	28.51525484530182	22.1447982121626
31	22.380081623824818	27.01367452944966	28.061964998954807	22.54427884777071
32	21.814689255105115	26.87473334436774	28.37881948130822	22.931757919218924
33	21.884258761636406	26.813934211645606	28.613905064989787	22.687901961728198
34	22.531551914348398	26.396846885758958	28.227349190784643	22.844252009108
35	22.179352166117475	26.523324908059447	28.73543738777384	22.561885538049246
36	21.966093602968474	26.682708317809332	28.172089241520926	23.179108837701264
37	22.327261552989214	25.90662914968918	28.553237817588094	23.21287147973351
38	21.818052330776297	25.867459209518955	29.13247814495384	23.18201031475091
39	21.870806458951684	25.85275399629007	29.16103131682877	23.11540822792949
40	21.332384638261644	25.76491837287805	29.73466651807587	23.168030470784434
41	20.881139014381432	25.87108605583101	29.928142283159104	23.319632646628452
42	21.21513858839185	25.861062771477688	29.71804896770063	23.205749672429835
43	20.855355434235715	25.531349470381524	29.696353832488498	23.916941262894262
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	760.0
1	637.5
2	515.0
3	1178.5
4	1842.0
5	1842.0
6	2528.0
7	3214.0
8	3098.5
9	2983.0
10	4545.0
11	6107.0
12	6107.0
13	10373.5
14	14640.0
15	18567.5
16	22495.0
17	21604.5
18	20714.0
19	20714.0
20	23540.0
21	26366.0
22	24305.5
23	22245.0
24	24279.5
25	26314.0
26	26314.0
27	29156.5
28	31999.0
29	36385.0
30	40771.0
31	45857.5
32	50944.0
33	50944.0
34	55645.5
35	60347.0
36	65553.5
37	70760.0
38	76471.0
39	82182.0
40	82182.0
41	85481.0
42	88780.0
43	89885.0
44	90990.0
45	93694.5
46	96399.0
47	96399.0
48	98180.0
49	99961.0
50	100262.5
51	100564.0
52	102851.0
53	105138.0
54	105138.0
55	98562.5
56	91987.0
57	87893.5
58	83800.0
59	77160.0
60	70520.0
61	70520.0
62	65092.0
63	59664.0
64	52797.0
65	45930.0
66	39798.5
67	33667.0
68	33667.0
69	28612.0
70	23557.0
71	20031.5
72	16506.0
73	13206.5
74	9907.0
75	9907.0
76	7773.0
77	5639.0
78	4573.5
79	3508.0
80	2818.5
81	2129.0
82	2129.0
83	1585.0
84	1041.0
85	899.5
86	758.0
87	637.5
88	517.0
89	517.0
90	387.5
91	258.0
92	149.5
93	41.0
94	24.0
95	7.0
96	7.0
97	4.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1516469.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.79466061519773
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.05893173581485	53.17982479521147
2	8.577774763918136	10.60121360739858
3	2.1907071634542477	4.0612201701881325
4	0.886785630691337	2.1919446834802097
5	0.5060838994187	1.5636641403697218
6	0.311565904558921	1.1551865598891367
7	0.2278909272318638	0.9857709753903022
8	0.15189378138768977	0.7508979736329061
9	0.11832502447317121	0.6580669256644124
>10	0.795009352267653	9.781787963304927
>50	0.10258929088976312	4.4282914608878885
>100	0.06987445332409185	7.756361518014733
>500	0.0017120483797427952	0.6812565667191891
>1k	4.280120949356988E-4	0.3573902497444644
>5k	4.280120949356988E-4	1.8471224101039063
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8580	0.5657880246810189	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7606	0.5015598736274859	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6580	0.4339027042425529	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5169	0.34085761067321524	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1593	0.10504665772923812	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.594266021923296E-5	0.0
2	0.0	0.0	0.0	8.572545828500286E-4	0.0
3	0.0	0.0	0.0	0.0013847958646038923	0.0
4	0.0	0.0	0.0	0.0027036490689885517	0.0
5	0.0	6.594266021923296E-5	0.0	0.0032971330109616485	0.0
6	0.0	6.594266021923296E-5	0.0	0.0033630756711808814	0.0
7	0.0	6.594266021923296E-5	0.0	0.0036927889722770464	0.0
8	0.0	6.594266021923296E-5	0.0	0.003956559613153978	0.0
9	0.0	1.3188532043846592E-4	0.0	0.006330495381046365	0.0
10	0.0	1.3188532043846592E-4	0.0	0.009429800411350315	0.0
11	0.0	4.615986215346308E-4	0.0	0.010616768295296508	0.0
12	0.0	4.615986215346308E-4	0.0	0.012463162781435031	0.0
13	0.0	4.615986215346308E-4	0.0	0.013452302684723525	0.0
14	0.0	4.615986215346308E-4	0.0	0.015496525151519747	0.0
15	0.0	4.615986215346308E-4	0.0	0.017936403579631367	0.0
16	0.0	4.615986215346308E-4	0.0	0.02176107787234688	0.0
17	0.0	4.615986215346308E-4	0.0	0.02795968793295478	0.0
18	0.0	4.615986215346308E-4	0.0	0.030597394341724097	0.0
19	0.0	5.934839419730968E-4	0.0	0.03593874981948197	0.0
20	0.0	5.934839419730968E-4	0.0	0.03910399751000515	0.0
21	0.0	5.934839419730968E-4	0.0	0.047214944716970804	0.0
22	0.0	5.934839419730968E-4	0.0	0.0640303230728752	0.0
23	0.0	5.934839419730968E-4	0.0	0.08552763030434515	0.0
24	6.594266021923296E-5	7.253692624115627E-4	0.0	0.11994969893878477	0.0
25	6.594266021923296E-5	7.253692624115627E-4	0.0	0.12931355668991584	0.0
26	6.594266021923296E-5	7.253692624115627E-4	0.0	0.14652459100713566	0.0
27	6.594266021923296E-5	7.913119226307956E-4	0.0	0.17778141195105207	0.0
28	6.594266021923296E-5	7.913119226307956E-4	0.0	0.2324478772727962	0.0
29	6.594266021923296E-5	7.913119226307956E-4	0.0	0.3061717713978987	0.0
30	6.594266021923296E-5	7.913119226307956E-4	0.0	0.41273511031217913	0.0
31	6.594266021923296E-5	7.913119226307956E-4	0.0	0.7332164389776513	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACTA	80	6.963128E-7	20.8125	5
CGAATTA	135	1.1532393E-9	17.814816	15
AACCGCG	95	3.6079236E-6	17.526316	7
GTCTAAT	100	5.88028E-6	16.650002	1
TCTAGCG	145	2.9813236E-9	16.586206	28
GGTATCA	4595	0.0	16.38629	1
GTATTGG	160	6.2937033E-10	16.1875	1
ACGGTAT	185	1.8189894E-11	16.0	9
ATACCGC	200	3.6379788E-12	15.725	27
ACGACGG	190	2.7284841E-11	15.578948	6
AGAACCG	145	5.3485564E-8	15.310345	5
TACGACG	195	4.1836756E-11	15.179486	5
CTAGCGG	160	1.0970325E-8	15.03125	29
CGACGGT	200	6.184564E-11	14.8	7
GACGGTA	200	6.184564E-11	14.8	8
CGGTCCA	190	4.5474735E-10	14.605264	10
CCTACAC	230	1.8189894E-12	14.478261	3
CGCAATA	180	3.3323886E-9	14.388889	36
AATCGGA	90	8.276161E-4	14.388889	27
CTTGCGC	245	0.0	14.34694	3
>>END_MODULE