FastQCFastQC Report
Fri 10 Feb 2017
ERR1630513.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630513.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265029
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT21710.8191556395715185No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT20130.7595395220900354RNA PCR Primer, Index 39 (95% over 23bp)
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA14520.5478645733108453No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12020.4535352734983719No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11670.44032917152462564No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC11540.435424047934377No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT11350.428255021148629No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC10940.41278501597938344No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC10330.3897686668251399No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG10120.38184500564089213No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC9270.3497730437046512No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC9040.34109474812190366No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC8940.3373215761294047No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT8290.31279595817816164No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC8280.31241864097891175RNA PCR Primer, Index 39 (95% over 21bp)
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA8280.31241864097891175No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG7990.30147644220066483No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC7910.29845790460666566No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC7290.27506423825317233No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC7220.27242301785842304No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7180.27091374906142346No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA7090.2675178942681744No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG7080.2671405770689245No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC7010.2644993566741753No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC6800.25657569548992754No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC6550.24714276550868017No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA6300.23770983552743286No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC5620.2120522659784401No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG5580.2105429971814405No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT5310.20035543280169343No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG5270.19884616400469382No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG5260.19846884680544394No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG5240.19771421240694417No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT5100.19243177161744562No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG4910.18526274483169766No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4610.17394322885420085No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC4400.1660195676699531RNA PCR Primer, Index 39 (95% over 24bp)
GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTA4340.16375566447445372No Hit
GGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT4260.16073712688045458No Hit
ATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC4190.15809590648570535No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGAT4170.15734127208720555No Hit
AGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGG4130.15583200329020597No Hit
GTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCG4050.15281346569620682No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT4010.15130419689920724No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC3990.15054956250070747No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG3890.14677639050820854No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC3790.1430032185157096No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC3740.14111663251946013No Hit
GTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGA3700.13960736372246055No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGC3680.13885272932396078No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC3660.138098094925461No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC3640.1373434605269612No Hit
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGA3550.13394760573371217No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC3540.13357028853446226No Hit
GACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGA3510.1324383369367126No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGAC3480.1313063853389629No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCG3450.1301744337412132No Hit
GGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAG3430.12941979934271344No Hit
GCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC3400.12828784774496377No Hit
ACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCG3350.1264012617487143No Hit
GCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGC3340.1260239445494644No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGG3330.1256466273502145No Hit
GGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTG3240.12225077255696545No Hit
CGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCC3210.12111882095921578No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCC3190.120364186560716No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTA3140.11847760056446653No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGC3130.11810028336521664No Hit
ACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAG3110.11734564896671686No Hit
ATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCAC3100.11696833176746695No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT3050.11508174577121749No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT3000.11319515977496802No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGC3000.11319515977496802No Hit
CCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTT3000.11319515977496802No Hit
GCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT2950.11130857377871856No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG2890.10904467058321919No Hit
GCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGA2880.1086673533839693No Hit
GGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACC2820.10640345018846993No Hit
GGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGC2820.10640345018846993No Hit
ACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTT2810.10602613298922005No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTC2790.10527149859072026No Hit
GGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCC2790.10527149859072026No Hit
CCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCG2760.1041395469929706No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGT2750.10376222979372068No Hit
ACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCCATTCG2720.10263027819597101No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGCT200.001839381637.035
CACGACA200.001839381637.019
AGTTGAG200.001839381637.017
TCGCTAT200.001839381637.01
CAGTGTC200.001839381637.029
ACTCGCC200.001839381637.035
GCCGAGT451.07464075E-732.8888912
GTAAGAT451.07464075E-732.888893
CCGTCTT2550.029.74509837
AAGTATT250.005488808729.622
CCTTTTA502.7074384E-729.620
CGCTATC250.005488808729.62
TTTGCCG502.7074384E-729.69
TGAGCGG250.005488808729.612
CGTCAAA250.005488808729.617
GACTCGC250.005488808729.634
TGACTCG250.005488808729.633
GCCGTCT2600.029.17307736
TTGCCGA604.296453E-827.74999810
TGCCGAG604.296453E-827.74999811
Produced by FastQC (version 0.11.5)