FastQCFastQC Report
Fri 10 Feb 2017
ERR1630512.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630512.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences921906
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42680.4629539237189041No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40790.44245291819339494No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34370.37281458196388784No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22980.24926619416730122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG2050.023.4634155
GACGGAC2200.021.8636367
CGGACCA2200.021.8636369
GTGCTAA603.725257E-521.5833321
TACGACG1400.021.1428595
TAGCGCA450.003825242420.5555554
TTACTCG450.003825242420.55555529
GACGGTA1450.020.4137948
CGCAAGA2450.020.3877562
ACGGACC2400.020.0416668
GCGCAAG2600.019.9230771
ACGACGG1700.019.5882346
AGACGGA2600.019.2115386
ACGGTAT1551.8189894E-1219.0967759
GCGAAAG2550.018.86274518
CGACGGT1700.018.4999987
CGAAAGC2700.017.81481619
ATCGTCT1705.456968E-1217.41176419
ATAGTAC852.7221695E-517.4117643
CAAGACG2900.017.2241384
Produced by FastQC (version 0.11.5)