##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630512.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	921906
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.547195701080152	31.0	31.0	34.0	30.0	34.0
2	31.7128828752606	31.0	31.0	34.0	30.0	34.0
3	31.809847207849824	31.0	31.0	34.0	30.0	34.0
4	35.48866695736876	37.0	35.0	37.0	33.0	37.0
5	35.41599251984476	37.0	35.0	37.0	33.0	37.0
6	35.45210140730183	37.0	35.0	37.0	33.0	37.0
7	35.43237271478871	37.0	35.0	37.0	33.0	37.0
8	35.42340216898469	37.0	35.0	37.0	33.0	37.0
9	37.06587656442197	39.0	37.0	39.0	33.0	39.0
10	36.89974249001525	39.0	37.0	39.0	33.0	39.0
11	37.044291934318686	39.0	37.0	39.0	33.0	39.0
12	36.95068586168221	39.0	37.0	39.0	33.0	39.0
13	37.036171800595724	39.0	37.0	39.0	33.0	39.0
14	38.17042952318349	40.0	38.0	41.0	33.0	41.0
15	38.12284658088786	40.0	37.0	41.0	33.0	41.0
16	38.097781118682384	40.0	37.0	41.0	33.0	41.0
17	38.084714710610406	40.0	37.0	41.0	33.0	41.0
18	38.07609994945255	40.0	37.0	41.0	33.0	41.0
19	38.09816510577	40.0	37.0	41.0	33.0	41.0
20	38.080182795209055	40.0	37.0	41.0	33.0	41.0
21	37.995128570591795	40.0	37.0	41.0	33.0	41.0
22	37.921947573830735	40.0	37.0	41.0	33.0	41.0
23	37.89405644393246	40.0	37.0	41.0	33.0	41.0
24	37.8339982601263	40.0	37.0	41.0	33.0	41.0
25	37.78372198467089	40.0	37.0	41.0	33.0	41.0
26	37.552076892871945	39.0	37.0	41.0	32.0	41.0
27	37.41411488806885	39.0	36.0	41.0	32.0	41.0
28	37.29816271940957	39.0	36.0	41.0	32.0	41.0
29	37.171137838347946	39.0	36.0	40.0	31.0	41.0
30	37.084230930268376	39.0	36.0	40.0	31.0	41.0
31	37.0188750263042	39.0	36.0	40.0	31.0	41.0
32	36.913564940460304	39.0	36.0	40.0	31.0	41.0
33	36.859456387093694	39.0	35.0	40.0	31.0	41.0
34	36.76847748035049	39.0	35.0	40.0	30.0	41.0
35	36.70968623699162	39.0	35.0	40.0	30.0	41.0
36	36.6597125954273	39.0	35.0	40.0	30.0	41.0
37	36.44325234893796	39.0	35.0	40.0	30.0	41.0
38	36.31084405568463	39.0	35.0	40.0	30.0	41.0
39	36.233847051651686	38.0	35.0	40.0	30.0	41.0
40	36.098414588906024	38.0	35.0	40.0	29.0	41.0
41	35.955469429638164	38.0	35.0	40.0	29.0	41.0
42	35.85374864682517	38.0	35.0	40.0	28.0	41.0
43	34.965983516757674	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	3.0
11	2.0
12	1.0
13	4.0
14	2.0
15	7.0
16	8.0
17	30.0
18	43.0
19	123.0
20	235.0
21	409.0
22	825.0
23	1286.0
24	2193.0
25	3586.0
26	5145.0
27	7515.0
28	10511.0
29	14236.0
30	18702.0
31	24180.0
32	30132.0
33	38665.0
34	49894.0
35	66011.0
36	93597.0
37	144009.0
38	241059.0
39	169490.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.07120248702145	20.36997264363178	12.729280425553146	23.82954444379362
2	19.023306063741856	21.829232047518946	34.31174111026504	24.835720778474162
3	20.756020678897848	24.437307057335563	29.367636179827443	25.439036083939143
4	14.568513492698823	15.996424798189837	35.11496833733591	34.32009337177543
5	14.110115347985586	37.86459790911438	33.96311554540268	14.062171197497358
6	34.09143665406235	35.405128071625526	15.84706032936113	14.656374944951004
7	28.520912110345307	31.17823292179463	21.90223298253835	18.398621985321714
8	27.674947337364113	32.84825134015833	20.308469627055253	19.16833169542231
9	26.867598215002396	13.663215121715231	20.810690026965872	38.6584966363165
10	17.296014995021185	27.010020544393896	32.875802956049746	22.81816150453517
11	35.047390948751826	22.296416337457398	21.692775619206298	20.96341709458448
12	21.87392207014598	26.40898312843175	29.225430792293356	22.491664009128915
13	29.80065212722338	20.28232813323701	25.876716281269456	24.04030345827015
14	22.647970617394833	21.589511295077806	26.23879224129141	29.523725846235948
15	25.499128978442485	28.15005000509813	23.29478276527108	23.056038251188298
16	24.448154150206204	26.843517668829577	25.25311691213638	23.455211268827842
17	22.893874212772236	26.826270791165257	26.34238197820602	23.937473017856483
18	22.848750306430375	25.2416189936935	28.330220217679457	23.579410482196668
19	23.406833234624788	25.81575561933646	28.234331916703002	22.543079229335746
20	24.218738135992172	25.034439519864282	27.789275696220656	22.957546647922893
21	24.73549364034945	25.466696170759274	27.194855006909595	22.602955181981677
22	24.2887018850078	25.441747857156805	27.595221204764915	22.674329053070487
23	23.611409406165055	25.40627786346981	27.526667577822465	23.45564515254267
24	23.272980108601093	26.11112195820398	27.532199595186498	23.08369833800843
25	23.580603662412436	25.847754543304845	27.32935895850553	23.242282835777182
26	23.214839690814465	26.47059461593698	27.457137712521668	22.857427980726886
27	23.73137825331433	25.443157979229987	27.39737023080444	23.428093536651243
28	22.921317357734953	25.792000485949764	28.023789844083886	23.262892312231397
29	22.63690658266678	26.3674387627372	28.115773191626914	22.87988146296911
30	22.85373996915087	26.55498499847056	27.80684798667109	22.78442704570748
31	23.078383262501816	26.764116949016493	27.16057819343838	22.99692159504331
32	22.48515575340653	26.23618893900246	27.45529370673366	23.823361600857353
33	22.257692215909213	26.21471169511859	28.05568029712357	23.471915791848627
34	23.479834169644192	25.5197384548967	27.845897521005398	23.154529854453706
35	23.03553724566279	25.709345638275487	28.032901402095224	23.2222157139665
36	22.409009161454637	26.582211201575866	27.574611728310693	23.4341679086588
37	22.970020804724125	25.58731584348079	28.038867303174076	23.403796048621007
38	22.593626682112927	25.50227463537497	28.221315405258235	23.682783277253865
39	22.82694764976039	25.380570253366397	28.291062212416453	23.501419884456766
40	22.084789555551218	25.381546491724755	28.943948732300257	23.589715220423773
41	21.458261471343064	25.62940256381887	29.227925623653604	23.68441034118446
42	22.070796805748092	25.58297700633253	29.16023976414081	23.185986423778562
43	21.7962568851922	25.011443682978523	29.04959941686029	24.142700014968987
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	326.0
1	287.5
2	249.0
3	552.5
4	856.0
5	856.0
6	1154.0
7	1452.0
8	1455.0
9	1458.0
10	1992.0
11	2526.0
12	2526.0
13	4387.5
14	6249.0
15	8356.5
16	10464.0
17	10279.5
18	10095.0
19	10095.0
20	11704.0
21	13313.0
22	12487.5
23	11662.0
24	13287.0
25	14912.0
26	14912.0
27	16954.0
28	18996.0
29	21539.0
30	24082.0
31	27001.5
32	29921.0
33	29921.0
34	33523.5
35	37126.0
36	40626.0
37	44126.0
38	47310.0
39	50494.0
40	50494.0
41	52321.5
42	54149.0
43	55208.0
44	56267.0
45	57129.5
46	57992.0
47	57992.0
48	59848.0
49	61704.0
50	60631.5
51	59559.0
52	60112.0
53	60665.0
54	60665.0
55	58773.5
56	56882.0
57	54583.5
58	52285.0
59	50161.5
60	48038.0
61	48038.0
62	44374.0
63	40710.0
64	36309.5
65	31909.0
66	27323.0
67	22737.0
68	22737.0
69	19469.0
70	16201.0
71	13640.0
72	11079.0
73	8420.5
74	5762.0
75	5762.0
76	4315.0
77	2868.0
78	2294.0
79	1720.0
80	1461.5
81	1203.0
82	1203.0
83	920.0
84	637.0
85	573.0
86	509.0
87	472.0
88	435.0
89	435.0
90	341.0
91	247.0
92	142.0
93	37.0
94	20.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	921906.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.80796046746119
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.91421715140802	60.737934253814494
2	5.558511818687873	7.427056756816305
3	1.2576280619198885	2.5205869753054126
4	0.5364713245312012	1.4336222016482811
5	0.30957588479798775	1.0341066736631643
6	0.21329017044652365	0.8549688765173649
7	0.14703722185992682	0.6876279833684319
8	0.11785764983433017	0.6299063368735852
9	0.09374851760990428	0.5636832532529018
>10	0.6901780592859249	9.398596352888266
>50	0.09464969312729013	4.520897194715888
>100	0.06407000470657503	7.78682663672637
>500	0.0021139848941592147	0.8743286483275431
>1k	6.504568905105276E-4	1.5298578560820653
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4268	0.4629539237189041	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4079	0.44245291819339494	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3437	0.37281458196388784	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2298	0.24926619416730122	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.508255722383843E-4	0.0
3	0.0	0.0	0.0	7.592965009447818E-4	0.0
4	0.0	0.0	0.0	0.001410122073183166	0.0
5	0.0	0.0	0.0	0.001627063930595961	0.0
6	0.0	0.0	0.0	0.0017355348593023584	0.0
7	0.0	0.0	0.0	0.0019524767167151532	0.0
8	0.0	0.0	0.0	0.0020609476454215504	0.0
9	0.0	0.0	0.0	0.0036880115760175116	0.0
10	0.0	1.084709287063974E-4	0.0	0.005532017364026268	0.0
11	0.0	1.084709287063974E-4	0.0	0.006399784793677446	0.0
12	0.0	1.084709287063974E-4	0.0	0.008026848724273408	0.0
13	0.0	1.084709287063974E-4	0.0	0.008569203367805395	0.0
14	0.0	1.084709287063974E-4	0.0	0.009653912654869368	0.0
15	0.0	1.084709287063974E-4	0.0	0.011172505656758931	0.0
16	0.0	1.084709287063974E-4	0.0	0.013558866088299674	0.0
17	0.0	1.084709287063974E-4	0.0	0.017246877664317185	0.0
18	0.0	1.084709287063974E-4	0.0	0.019307825309738735	0.0
19	0.0	1.084709287063974E-4	0.0	0.021911127598692275	0.0
20	0.0	1.084709287063974E-4	0.0	0.024297488030233017	0.0
21	0.0	1.084709287063974E-4	0.0	0.029504092608140093	0.0
22	0.0	1.084709287063974E-4	0.0	0.038724121548183874	0.0
23	0.0	1.084709287063974E-4	0.0	0.05184910392165796	0.0
24	0.0	1.084709287063974E-4	0.0	0.07137387108880949	0.0
25	0.0	1.084709287063974E-4	0.0	0.07647200473801016	0.0
26	0.0	1.084709287063974E-4	0.0	0.08525814996322835	0.0
27	0.0	1.084709287063974E-4	0.0	0.09979325440988561	0.0
28	0.0	1.084709287063974E-4	0.0	0.13038205630508967	0.0
29	0.0	1.084709287063974E-4	0.0	0.17767538122107893	0.0
30	0.0	1.084709287063974E-4	0.0	0.25338808945814434	0.0
31	0.0	1.084709287063974E-4	0.0	0.5051491149856927	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	205	0.0	23.463415	5
GACGGAC	220	0.0	21.863636	7
CGGACCA	220	0.0	21.863636	9
GTGCTAA	60	3.725257E-5	21.583332	1
TACGACG	140	0.0	21.142859	5
TAGCGCA	45	0.0038252424	20.555555	4
TTACTCG	45	0.0038252424	20.555555	29
GACGGTA	145	0.0	20.413794	8
CGCAAGA	245	0.0	20.387756	2
ACGGACC	240	0.0	20.041666	8
GCGCAAG	260	0.0	19.923077	1
ACGACGG	170	0.0	19.588234	6
AGACGGA	260	0.0	19.211538	6
ACGGTAT	155	1.8189894E-12	19.096775	9
GCGAAAG	255	0.0	18.862745	18
CGACGGT	170	0.0	18.499998	7
CGAAAGC	270	0.0	17.814816	19
ATCGTCT	170	5.456968E-12	17.411764	19
ATAGTAC	85	2.7221695E-5	17.411764	3
CAAGACG	290	0.0	17.224138	4
>>END_MODULE