FastQCFastQC Report
Fri 10 Feb 2017
ERR1630511.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630511.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1592868
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA28960.18181041994691335No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC27860.17490463742130546No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC19320.12129065308613142No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC18740.1176494222999018No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17530.11005306152173312No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG17500.10986472199830746No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT17350.10892302438117911No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC17100.10735352835263186No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA16730.10503067423038193No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC16390.10289615963155767No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC16180.10157778296757797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11250.022.0355551
GGATCGA705.103964E-621.14285719
TTAACGG701.21957215E-418.535
CTAATCG500.00703604618.49999826
GACGGAC4750.017.9157897
ACGGACC4900.017.3673488
CAAGACG5450.016.9724774
TCTAGCG2950.016.93220328
AAGACGG5100.016.6862755
CTAGACC904.447691E-516.4444453
GTAGACC2250.016.4444454
AGACGGA5250.016.2095246
CTAGCGG3150.015.85714229
CGAACGA2700.015.75925916
TAGGAGT2003.6379788E-1215.7249994
ATAACGA3550.015.63380212
TAACGGC957.062411E-515.57894836
CGCAATA3100.015.516129536
CGAAAGC5650.015.06194719
GCGAAAG5650.015.06194718
Produced by FastQC (version 0.11.5)