##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630511.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1592868
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.914881835783003	33.0	31.0	34.0	30.0	34.0
2	32.096987948781695	33.0	31.0	34.0	30.0	34.0
3	32.21222536958493	34.0	31.0	34.0	30.0	34.0
4	35.808095837194294	37.0	35.0	37.0	35.0	37.0
5	35.77254612434929	37.0	35.0	37.0	35.0	37.0
6	35.79328105028163	37.0	35.0	37.0	35.0	37.0
7	35.776590401715644	37.0	35.0	37.0	35.0	37.0
8	35.75489494421383	37.0	35.0	37.0	33.0	37.0
9	37.48230926856463	39.0	37.0	39.0	35.0	39.0
10	37.37273019484351	39.0	37.0	39.0	34.0	39.0
11	37.47977547417614	39.0	37.0	39.0	35.0	39.0
12	37.39626698508602	39.0	37.0	39.0	34.0	39.0
13	37.44532943093841	39.0	37.0	39.0	35.0	39.0
14	38.70862117890497	40.0	38.0	41.0	35.0	41.0
15	38.68030433155792	40.0	38.0	41.0	35.0	41.0
16	38.666847472609156	40.0	38.0	41.0	34.0	41.0
17	38.636381671299816	40.0	38.0	41.0	34.0	41.0
18	38.6149002930563	40.0	38.0	41.0	34.0	41.0
19	38.616639294655926	40.0	38.0	41.0	34.0	41.0
20	38.594820160867066	40.0	38.0	41.0	34.0	41.0
21	38.52165276720984	40.0	38.0	41.0	34.0	41.0
22	38.46173317563037	40.0	38.0	41.0	34.0	41.0
23	38.425391808988564	40.0	38.0	41.0	34.0	41.0
24	38.37668532483546	40.0	38.0	41.0	34.0	41.0
25	38.33358508049631	40.0	38.0	41.0	34.0	41.0
26	38.14950265809848	40.0	38.0	41.0	33.0	41.0
27	38.03332102848447	40.0	38.0	41.0	33.0	41.0
28	37.90972698302684	40.0	37.0	41.0	33.0	41.0
29	37.81535632582235	40.0	37.0	41.0	33.0	41.0
30	37.749638388115024	40.0	37.0	41.0	32.0	41.0
31	37.73217680309982	40.0	37.0	41.0	32.0	41.0
32	37.65005135391005	40.0	37.0	41.0	32.0	41.0
33	37.601960739998546	40.0	37.0	41.0	32.0	41.0
34	37.543053787256696	40.0	37.0	41.0	32.0	41.0
35	37.509543163651976	40.0	37.0	41.0	32.0	41.0
36	37.48186478728934	40.0	37.0	41.0	32.0	41.0
37	37.29768380054091	40.0	36.0	41.0	31.0	41.0
38	37.18699540702682	39.0	36.0	41.0	31.0	41.0
39	37.137373592789864	39.0	36.0	41.0	31.0	41.0
40	37.050227639703984	39.0	36.0	41.0	31.0	41.0
41	36.928378246031684	39.0	35.0	41.0	31.0	41.0
42	36.83404400113506	39.0	35.0	41.0	31.0	41.0
43	35.94659758372947	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	5.0
11	5.0
12	0.0
13	1.0
14	3.0
15	7.0
16	10.0
17	27.0
18	38.0
19	108.0
20	248.0
21	480.0
22	899.0
23	1633.0
24	2627.0
25	4179.0
26	6155.0
27	9279.0
28	12886.0
29	17909.0
30	24136.0
31	31849.0
32	41076.0
33	52868.0
34	70404.0
35	94908.0
36	134045.0
37	203333.0
38	378639.0
39	505106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.053619006722464	18.3245567115417	11.492603279116663	26.129221002619175
2	20.393591936054964	19.946787806648132	32.59259398770018	27.067026269596727
3	21.306975844828322	22.148100156447363	27.651506590627722	28.89341740809659
4	15.125861025521262	14.617344312271952	32.80786606297571	37.448928599231074
5	15.743865781722025	35.660895943668905	33.01095884906972	15.584279425539341
6	37.10288611485698	33.00844765542405	14.820374318524824	15.068291911194148
7	31.078155879834362	28.76748104676596	20.28504559072064	19.86931748267904
8	28.79836872860777	31.344467965958263	19.019027314253282	20.838135991180685
9	27.421355693001555	13.181820464721497	18.72490375850353	40.67192008377342
10	18.21280859430913	24.99045746414643	31.350808729913588	25.445925211630843
11	38.556051097768304	20.31335929907563	19.991298714017734	21.139290889138334
12	23.235572564707184	24.015235411848316	27.242119246541456	25.507072776903044
13	31.044443105141166	18.593568330834696	23.955720122445804	26.406268441578334
14	23.97681414906948	19.733587466130274	23.2633212544919	33.02627713030835
15	27.148640063081185	26.5811102991585	20.664173051376512	25.606076586383807
16	27.638573943352498	24.447600177792513	22.222180368994795	25.6916455098602
17	25.66176230547666	24.882538917223524	23.30695324408551	26.148745533214303
18	26.043777638825063	23.037439386063376	24.900368392107822	26.01841458300374
19	25.809294932160103	24.00738793170558	24.07952196917761	26.10379516695671
20	26.16626110889289	23.340666018778705	24.27451615576432	26.218556716564084
21	27.320029029398547	23.47237812549439	24.01680490787686	25.1907879372302
22	26.850059138610355	23.34939241669743	24.055728409384834	25.74482003530738
23	25.880236152650436	23.28366192302187	24.50906164227042	26.32704028205727
24	25.75103523958043	24.173377831684732	23.975119093358646	26.10046783537619
25	26.630141355090313	23.603525213639802	23.868707262623143	25.89762616864674
26	26.277067528508326	24.469008103621896	23.954778424828675	25.299145943041108
27	27.0966583546157	23.144541795051442	23.890429087658234	25.86837076267462
28	25.697797934292105	23.614072226951638	24.676934937483836	26.01119490127242
29	25.14407973542064	23.912715931263605	24.756288656687182	26.18691567662857
30	24.813167192761735	24.565500719456978	24.939668572662644	25.68166351511864
31	26.089795262382072	24.364102989073796	23.651112333225353	25.894989415318783
32	25.739358189128037	23.686771282993945	23.872160153885947	26.70171037399207
33	25.064286557329297	23.724815866725933	24.489348772151867	26.721548803792906
34	26.427174128678583	23.596996110161044	24.01379147550205	25.962038285658323
35	25.932155081274782	23.427051080189948	25.28269762466193	25.35809621387334
36	25.324760118226997	24.36328685113895	24.273825577511758	26.038127453122296
37	26.32339905127104	23.416064607990116	24.661114417516078	25.599421923222764
38	25.184635512798298	23.232621912173514	25.105784032324085	26.476958542704104
39	25.78958206204155	23.36634297380574	24.74184929322455	26.102225670928163
40	25.411772978049658	23.39704231612412	25.239128414909455	25.952056290916765
41	24.595446703681663	23.65833201495667	26.090046381746635	25.656174899615035
42	25.638784883618733	23.336648108945628	25.87540210488251	25.149164902553128
43	24.644666099136902	22.6335766679976	25.811178327394362	26.910578905471137
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	38.0
1	45.5
2	53.0
3	168.0
4	283.0
5	283.0
6	384.0
7	485.0
8	466.0
9	447.0
10	625.0
11	803.0
12	803.0
13	1447.5
14	2092.0
15	2930.5
16	3769.0
17	3967.5
18	4166.0
19	4166.0
20	5440.0
21	6714.0
22	7126.5
23	7539.0
24	9038.5
25	10538.0
26	10538.0
27	12886.0
28	15234.0
29	18546.0
30	21858.0
31	25688.0
32	29518.0
33	29518.0
34	35225.0
35	40932.0
36	47118.5
37	53305.0
38	60906.0
39	68507.0
40	68507.0
41	75051.0
42	81595.0
43	86001.5
44	90408.0
45	95995.0
46	101582.0
47	101582.0
48	108563.5
49	115545.0
50	115782.5
51	116020.0
52	120610.0
53	125200.0
54	125200.0
55	122893.0
56	120586.0
57	116162.0
58	111738.0
59	109153.5
60	106569.0
61	106569.0
62	102012.0
63	97455.0
64	89448.5
65	81442.0
66	73178.5
67	64915.0
68	64915.0
69	54461.5
70	44008.0
71	37560.0
72	31112.0
73	23456.0
74	15800.0
75	15800.0
76	12269.0
77	8738.0
78	7034.0
79	5330.0
80	4571.0
81	3812.0
82	3812.0
83	2811.5
84	1811.0
85	1562.5
86	1314.0
87	1126.5
88	939.0
89	939.0
90	742.0
91	545.0
92	308.5
93	72.0
94	53.0
95	34.0
96	34.0
97	25.0
98	16.0
99	8.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1592868.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.633614178987784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.0904460604923	43.41893648026443
2	8.532478428555253	8.811253983411158
3	2.648933430046642	4.103220201385531
4	1.2555672499524002	2.593178998392598
5	0.7316492714533247	1.8888848098279232
6	0.47476710572880215	1.4708364937245402
7	0.33482118248379716	1.210161942872459
8	0.266111423212749	1.0992235643830732
9	0.18573236921791864	0.8631030143472586
>10	1.2318275406501176	12.249315341754828
>50	0.1322924229775904	4.833039050700022
>100	0.10073850257237402	10.439911240454416
>500	0.009756675104543863	3.4301862581028812
>1k	0.004878337552271931	3.588748620378969
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2896	0.18181041994691335	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2786	0.17490463742130546	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1932	0.12129065308613142	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	1874	0.1176494222999018	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1753	0.11005306152173312	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1750	0.10986472199830746	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1735	0.10892302438117911	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1710	0.10735352835263186	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	1673	0.10503067423038193	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	1639	0.10289615963155767	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1618	0.10157778296757797	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.277984114188998E-5	0.0	7.533580937026797E-4	0.0
3	0.0	6.277984114188998E-5	0.0	9.416976171283496E-4	0.0
4	0.0	6.277984114188998E-5	0.0	0.0015694960285472493	0.0
5	0.0	6.277984114188998E-5	0.0	0.0015694960285472493	0.0
6	0.0	6.277984114188998E-5	0.0	0.0015694960285472493	0.0
7	0.0	6.277984114188998E-5	0.0	0.0016950557108310292	0.0
8	0.0	6.277984114188998E-5	0.0	0.0018206153931148092	0.0
9	0.0	6.277984114188998E-5	0.0	0.002825092851385049	0.0
10	0.0	6.277984114188998E-5	0.0	0.0042062493565066285	0.0
11	0.0	6.277984114188998E-5	0.0	0.0045829284033579686	0.0
12	0.0	6.277984114188998E-5	0.0	0.005022387291351198	0.0
13	0.0	6.277984114188998E-5	0.0	0.005085167132493088	0.0
14	0.0	6.277984114188998E-5	0.0	0.005399066338202538	0.0
15	0.0	6.277984114188998E-5	0.0	0.005838525226195768	0.0
16	0.0	6.277984114188998E-5	0.0	0.007156901890175457	0.0
17	0.0	6.277984114188998E-5	0.0	0.008161379348445697	0.0
18	0.0	6.277984114188998E-5	0.0	0.008726397918722706	0.0
19	0.0	6.277984114188998E-5	0.0	0.009479756012425386	0.0
20	0.0	6.277984114188998E-5	0.0	0.010421453629553736	0.0
21	0.0	6.277984114188998E-5	0.0	0.012242069022668545	0.0
22	0.0	6.277984114188998E-5	0.0	0.014062684415783355	0.0
23	0.0	6.277984114188998E-5	0.0	0.01726445631401974	0.0
24	0.0	6.277984114188998E-5	0.0	0.0212823661471007	0.0
25	0.0	6.277984114188998E-5	0.0	0.02297742185793173	0.0
26	0.0	6.277984114188998E-5	0.0	0.02611641391502623	0.0
27	0.0	6.277984114188998E-5	0.0	0.0325827375526409	0.0
28	0.0	6.277984114188998E-5	0.0	0.04670820180956614	0.0
29	0.0	6.277984114188998E-5	0.0	0.06899504541493708	0.0
30	0.0	6.277984114188998E-5	0.0	0.10509345407152382	0.0
31	0.0	6.277984114188998E-5	0.0	0.2211106005017365	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1125	0.0	22.035555	1
GGATCGA	70	5.103964E-6	21.142857	19
TTAACGG	70	1.21957215E-4	18.5	35
CTAATCG	50	0.007036046	18.499998	26
GACGGAC	475	0.0	17.915789	7
ACGGACC	490	0.0	17.367348	8
CAAGACG	545	0.0	16.972477	4
TCTAGCG	295	0.0	16.932203	28
AAGACGG	510	0.0	16.686275	5
CTAGACC	90	4.447691E-5	16.444445	3
GTAGACC	225	0.0	16.444445	4
AGACGGA	525	0.0	16.209524	6
CTAGCGG	315	0.0	15.857142	29
CGAACGA	270	0.0	15.759259	16
TAGGAGT	200	3.6379788E-12	15.724999	4
ATAACGA	355	0.0	15.633802	12
TAACGGC	95	7.062411E-5	15.578948	36
CGCAATA	310	0.0	15.5161295	36
CGAAAGC	565	0.0	15.061947	19
GCGAAAG	565	0.0	15.061947	18
>>END_MODULE